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The map label for this gene is thiI

Identifier: 23099653

GI number: 23099653

Start: 2231324

End: 2232526

Strand: Reverse

Name: thiI

Synonym: OB2198

Alternate gene names: 23099653

Gene position: 2232526-2231324 (Counterclockwise)

Preceding gene: 23099654

Following gene: 23099652

Centisome position: 61.49

GC content: 34.66

Gene sequence:

>1203_bases
ATGAATTACGATCATATATTAATACGCTATGGAGAAATGGCGTTAAAAGGAAAGAACATAAAACAATTTATCATAAAATT
ACAGGAAAATATTCAACAAAAAATCAAACATTTTGAGGGTGTAAAAGTAAAACGAACTCAAGGTAGGATGTTTGTGTTAT
TAAATGGCAATGAACCTGAACCAATTATAGAAAAACTAAAAAATGTATTCGGTATTCATAGTTTAAGTATAGCGATTCGA
GTAGAGAACACTGAAGAGCAAATAAAAGAAGGAGCATTATATGCTTTAAAGAGAGAAGCGAACGCAAAAACTTTTAAAGT
AACCGTGAAGCGTATTTCAAAGGACTTTCCTATTCCTTCCCAACAAATGAACCAGATTTTAGGTGGACATTTATTAGCCA
ATACTAATGATATTACTGTAAATGTACATGAACCTGATGTCGAAGTACGTGTGGAGATTCGTAAAGAAGCAACGTATATT
ACCTCAGGAACTATCCGTTGTAGAGGCGGTCTACCTGTAGGGACATCTGGTAAATCATTATTATTATTATCTGGTGGGAT
AGACAGTCCTGTAGCTGGATTTTTGGCAATGAAAAGAGGGGTTCAATTAGAAGCTATCCATTTTCATTCGCCACCATTTA
CAAGTGAGAGAGCAAAACAAAAAGTTCTTGATCTAACACAAACGCTAACGAAATATGGGAAATCCATAAAAGTGCATATT
GTGCCATTTACGAAGTTACAACAAGAAATCTTTCGAGAGATGCCAAATGATTATGCAATGACAATTATGCGTAGAATGAT
GTTTCGTATTAGTGAAAGAATTTGTGAGCAAGAAGAGATATTATCGCTGACAACGGGAGAAAATTTAGGCCAAGTAGCTA
GTCAGACAATGCAAAGCATGCATACGATTAATGAAGTGACAAATTATCCGATAATTCGTCCGCTAATATCCATGGATAAA
CAAGAAGTTATAGAGGTGTCCCAAGAGATAGGGACATATGAGACATCCATTCAACCTTATGAGGATTGCTGTACGATATT
TGTACCGAAATCTCCTAAAACAAAACCGAAGCGGGAAAAAGTTAATTATTATGAGTCTCATCATGATTTTACTCCAACAA
TGGAAGAAACAATCAATGGGATTGAGACAGTTAAAATTACGGATAAAACTGTAATAGACCAATCTTTTAAAGACCTTCTG
TAA

Upstream 100 bases:

>100_bases
TAGTTTTTCTTATGATAATACGGTGGCAGAAGTAGAACTGTTTTTAAAAGAACTGCATAATGCAATTAAACAATATAAAG
AAGCATTGGGGTAGATAGTA

Downstream 100 bases:

>100_bases
AAGAGATAAAATGCTAAATACAAAAAGAAAAAACTACCAATAAATTAGATGCATGGTTTCCCTCCAAACAACACATTCTA
TAAGTACCAAGGAGGTGAAC

Product: thiamine biosynthesis

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNYDHILIRYGEMALKGKNIKQFIIKLQENIQQKIKHFEGVKVKRTQGRMFVLLNGNEPEPIIEKLKNVFGIHSLSIAIR
VENTEEQIKEGALYALKREANAKTFKVTVKRISKDFPIPSQQMNQILGGHLLANTNDITVNVHEPDVEVRVEIRKEATYI
TSGTIRCRGGLPVGTSGKSLLLLSGGIDSPVAGFLAMKRGVQLEAIHFHSPPFTSERAKQKVLDLTQTLTKYGKSIKVHI
VPFTKLQQEIFREMPNDYAMTIMRRMMFRISERICEQEEILSLTTGENLGQVASQTMQSMHTINEVTNYPIIRPLISMDK
QEVIEVSQEIGTYETSIQPYEDCCTIFVPKSPKTKPKREKVNYYESHHDFTPTMEETINGIETVKITDKTVIDQSFKDLL

Sequences:

>Translated_400_residues
MNYDHILIRYGEMALKGKNIKQFIIKLQENIQQKIKHFEGVKVKRTQGRMFVLLNGNEPEPIIEKLKNVFGIHSLSIAIR
VENTEEQIKEGALYALKREANAKTFKVTVKRISKDFPIPSQQMNQILGGHLLANTNDITVNVHEPDVEVRVEIRKEATYI
TSGTIRCRGGLPVGTSGKSLLLLSGGIDSPVAGFLAMKRGVQLEAIHFHSPPFTSERAKQKVLDLTQTLTKYGKSIKVHI
VPFTKLQQEIFREMPNDYAMTIMRRMMFRISERICEQEEILSLTTGENLGQVASQTMQSMHTINEVTNYPIIRPLISMDK
QEVIEVSQEIGTYETSIQPYEDCCTIFVPKSPKTKPKREKVNYYESHHDFTPTMEETINGIETVKITDKTVIDQSFKDLL
>Mature_400_residues
MNYDHILIRYGEMALKGKNIKQFIIKLQENIQQKIKHFEGVKVKRTQGRMFVLLNGNEPEPIIEKLKNVFGIHSLSIAIR
VENTEEQIKEGALYALKREANAKTFKVTVKRISKDFPIPSQQMNQILGGHLLANTNDITVNVHEPDVEVRVEIRKEATYI
TSGTIRCRGGLPVGTSGKSLLLLSGGIDSPVAGFLAMKRGVQLEAIHFHSPPFTSERAKQKVLDLTQTLTKYGKSIKVHI
VPFTKLQQEIFREMPNDYAMTIMRRMMFRISERICEQEEILSLTTGENLGQVASQTMQSMHTINEVTNYPIIRPLISMDK
QEVIEVSQEIGTYETSIQPYEDCCTIFVPKSPKTKPKREKVNYYESHHDFTPTMEETINGIETVKITDKTVIDQSFKDLL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=369, Percent_Identity=30.8943089430894, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_OCEIH (Q8EPB3)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693119.1
- ProteinModelPortal:   Q8EPB3
- SMR:   Q8EPB3
- GeneID:   1015557
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2198
- NMPDR:   fig|221109.1.peg.2196
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- BioCyc:   OIHE221109:OB2198-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 45597; Mature: 45597

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS51165 THUMP

Important sites: BINDING 264-264 BINDING 286-286 BINDING 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYDHILIRYGEMALKGKNIKQFIIKLQENIQQKIKHFEGVKVKRTQGRMFVLLNGNEPE
CCCCEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCC
PIIEKLKNVFGIHSLSIAIRVENTEEQIKEGALYALKREANAKTFKVTVKRISKDFPIPS
HHHHHHHHHHCEEEEEEEEEECCCHHHHHCCHHHEEHHCCCCCEEEHHHHHHHCCCCCCH
QQMNQILGGHLLANTNDITVNVHEPDVEVRVEIRKEATYITSGTIRCRGGLPVGTSGKSL
HHHHHHHCCEEEECCCEEEEEEECCCEEEEEEEEECEEEEECCEEEECCCCCCCCCCCEE
LLLSGGIDSPVAGFLAMKRGVQLEAIHFHSPPFTSERAKQKVLDLTQTLTKYGKSIKVHI
EEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEE
VPFTKLQQEIFREMPNDYAMTIMRRMMFRISERICEQEEILSLTTGENLGQVASQTMQSM
EEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCHHHHHHHHHHHHH
HTINEVTNYPIIRPLISMDKQEVIEVSQEIGTYETSIQPYEDCCTIFVPKSPKTKPKREK
HHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHH
VNYYESHHDFTPTMEETINGIETVKITDKTVIDQSFKDLL
HHHHHCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MNYDHILIRYGEMALKGKNIKQFIIKLQENIQQKIKHFEGVKVKRTQGRMFVLLNGNEPE
CCCCEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCC
PIIEKLKNVFGIHSLSIAIRVENTEEQIKEGALYALKREANAKTFKVTVKRISKDFPIPS
HHHHHHHHHHCEEEEEEEEEECCCHHHHHCCHHHEEHHCCCCCEEEHHHHHHHCCCCCCH
QQMNQILGGHLLANTNDITVNVHEPDVEVRVEIRKEATYITSGTIRCRGGLPVGTSGKSL
HHHHHHHCCEEEECCCEEEEEEECCCEEEEEEEEECEEEEECCEEEECCCCCCCCCCCEE
LLLSGGIDSPVAGFLAMKRGVQLEAIHFHSPPFTSERAKQKVLDLTQTLTKYGKSIKVHI
EEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEE
VPFTKLQQEIFREMPNDYAMTIMRRMMFRISERICEQEEILSLTTGENLGQVASQTMQSM
EEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCHHHHHHHHHHHHH
HTINEVTNYPIIRPLISMDKQEVIEVSQEIGTYETSIQPYEDCCTIFVPKSPKTKPKREK
HHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHH
VNYYESHHDFTPTMEETINGIETVKITDKTVIDQSFKDLL
HHHHHCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376