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The map label for this gene is ackA

Identifier: 23099646

GI number: 23099646

Start: 2225834

End: 2227027

Strand: Reverse

Name: ackA

Synonym: OB2191

Alternate gene names: 23099646

Gene position: 2227027-2225834 (Counterclockwise)

Preceding gene: 23099647

Following gene: 23099644

Centisome position: 61.34

GC content: 36.52

Gene sequence:

>1194_bases
ATGAGTAATGTGTTAGCAATCAATGCTGGAAGTTCTTCATTAAAGTTTCAACTTATTAAAATGCCTGAAGAACAAGTAAT
TGCAAAAGGACTAGTAGAACGTATTGGAATGCCTGATGCAATTTTTTCTGTAGAAGCAGATGGTGAGAAAGATAAAAAAG
TAACAGAGATTGCAGATCATAGTGTTGCTGTAAAAATGTTATTGGAGTCATTAACTTCTACAGGGGTAATTCAATCATTT
GATGAAATAGATGCTGTAGGTCATCGTGTGGTACATGGTGGCGAGTATTTCTCTGATTCCGTATTGATTACGGACGAAAT
CATTCAACAAATAGAAGAAATATCTGAATTAGCTCCGTTGCATAACCCTGCAAACTTAACAGGAATTCATGCATTTAAGG
AAATCTTACCTGAAATTCCGATGGTAGCTGTTTTTGATACTGCATTCCACCAATCAATGCCAGAGGCTTCTTATATGTAC
AGCCTACCTAGAGAATACTACGAAGAGTATGGGATTCGTAAATATGGATTCCACGGTACATCTCACAAGTATGTTTCCGA
ACGTGCAGCAGATTTATTAGGGGTACCTTTATCTAATCTACGTTTAATCTCTTGTCACATTGGTAATGGGGCAAGTGTAA
CTGCAATTAAAGATGGTGAGTCCATTGATACTTCTATGGGATTCACACCATTAGCAGGTGTAACGATGGGTACTCGTTCA
GGGAATATTGACCCCGCTTTAATTCCATATATTATGGACAAGACTGGAAAAACAGCAGATGAAGTGTTGAATGTATTGAA
CAAAGAAAGTGGTATGTTAGCATTATCTGGATTCTCTAGTGATTTACGCGATATTGAAGAAAAATCTGAAACAGATGCTA
GAGCGGAATTAGCATTAGATGTATTCGCAGGTCGTATCCATAAATATATTGGCTCTTATGCTGCAAAAATGAATGGACTT
GACGCAATCATTTTCACTGCTGGAGTTGGTGAAAACAGTGTAACTATCCGTGAAAAAATCCTAACTGGACTTGAATTCAT
GGGAATCTACTGGGATCCAAAATTAAATGATGTACGTGGTAAAGAACAATTTATAAACTATCCACACTCTCCAGTAAAAG
TAATCATTATTCCAACAAATGAAGAAGCAATGATTGCAAAAGATACAGTGAGATTAAGCGGATTAAATAGTTAA

Upstream 100 bases:

>100_bases
CTTTCAAAAATGCTCATGCAATGGCGGACATCTTAAAGAAAATGGACCAATGGTTTGCATCCTATCCAAAAGGGATTAAT
AGTAAATAGGAGTGAGAGTA

Downstream 100 bases:

>100_bases
TTCGTCTGTAAAATAAATGACAAAAATAGACCTTTTTCTTATCTTCGGATAGGAAGAAGGTCTATTTAGTTTATAATTAT
TTAAATTTCGCTTTAATTGA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSNVLAINAGSSSLKFQLIKMPEEQVIAKGLVERIGMPDAIFSVEADGEKDKKVTEIADHSVAVKMLLESLTSTGVIQSF
DEIDAVGHRVVHGGEYFSDSVLITDEIIQQIEEISELAPLHNPANLTGIHAFKEILPEIPMVAVFDTAFHQSMPEASYMY
SLPREYYEEYGIRKYGFHGTSHKYVSERAADLLGVPLSNLRLISCHIGNGASVTAIKDGESIDTSMGFTPLAGVTMGTRS
GNIDPALIPYIMDKTGKTADEVLNVLNKESGMLALSGFSSDLRDIEEKSETDARAELALDVFAGRIHKYIGSYAAKMNGL
DAIIFTAGVGENSVTIREKILTGLEFMGIYWDPKLNDVRGKEQFINYPHSPVKVIIIPTNEEAMIAKDTVRLSGLNS

Sequences:

>Translated_397_residues
MSNVLAINAGSSSLKFQLIKMPEEQVIAKGLVERIGMPDAIFSVEADGEKDKKVTEIADHSVAVKMLLESLTSTGVIQSF
DEIDAVGHRVVHGGEYFSDSVLITDEIIQQIEEISELAPLHNPANLTGIHAFKEILPEIPMVAVFDTAFHQSMPEASYMY
SLPREYYEEYGIRKYGFHGTSHKYVSERAADLLGVPLSNLRLISCHIGNGASVTAIKDGESIDTSMGFTPLAGVTMGTRS
GNIDPALIPYIMDKTGKTADEVLNVLNKESGMLALSGFSSDLRDIEEKSETDARAELALDVFAGRIHKYIGSYAAKMNGL
DAIIFTAGVGENSVTIREKILTGLEFMGIYWDPKLNDVRGKEQFINYPHSPVKVIIIPTNEEAMIAKDTVRLSGLNS
>Mature_396_residues
SNVLAINAGSSSLKFQLIKMPEEQVIAKGLVERIGMPDAIFSVEADGEKDKKVTEIADHSVAVKMLLESLTSTGVIQSFD
EIDAVGHRVVHGGEYFSDSVLITDEIIQQIEEISELAPLHNPANLTGIHAFKEILPEIPMVAVFDTAFHQSMPEASYMYS
LPREYYEEYGIRKYGFHGTSHKYVSERAADLLGVPLSNLRLISCHIGNGASVTAIKDGESIDTSMGFTPLAGVTMGTRSG
NIDPALIPYIMDKTGKTADEVLNVLNKESGMLALSGFSSDLRDIEEKSETDARAELALDVFAGRIHKYIGSYAAKMNGLD
AIIFTAGVGENSVTIREKILTGLEFMGIYWDPKLNDVRGKEQFINYPHSPVKVIIIPTNEEAMIAKDTVRLSGLNS

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=396, Percent_Identity=44.9494949494949, Blast_Score=337, Evalue=6e-94,
Organism=Escherichia coli, GI145693184, Length=399, Percent_Identity=42.8571428571429, Blast_Score=332, Evalue=3e-92,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_OCEIH (Q8EPB9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693112.1
- ProteinModelPortal:   Q8EPB9
- SMR:   Q8EPB9
- GeneID:   1017605
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2191
- NMPDR:   fig|221109.1.peg.2189
- HOGENOM:   HBG285863
- OMA:   ENSVEVR
- ProtClustDB:   PRK00180
- BioCyc:   OIHE221109:OB2191-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43349; Mature: 43218

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVLAINAGSSSLKFQLIKMPEEQVIAKGLVERIGMPDAIFSVEADGEKDKKVTEIADH
CCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHH
SVAVKMLLESLTSTGVIQSFDEIDAVGHRVVHGGEYFSDSVLITDEIIQQIEEISELAPL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHHHHCCC
HNPANLTGIHAFKEILPEIPMVAVFDTAFHQSMPEASYMYSLPREYYEEYGIRKYGFHGT
CCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCC
SHKYVSERAADLLGVPLSNLRLISCHIGNGASVTAIKDGESIDTSMGFTPLAGVTMGTRS
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCCC
GNIDPALIPYIMDKTGKTADEVLNVLNKESGMLALSGFSSDLRDIEEKSETDARAELALD
CCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHH
VFAGRIHKYIGSYAAKMNGLDAIIFTAGVGENSVTIREKILTGLEFMGIYWDPKLNDVRG
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCC
KEQFINYPHSPVKVIIIPTNEEAMIAKDTVRLSGLNS
HHHHHCCCCCCEEEEEEECCCCCEEEECEEEECCCCC
>Mature Secondary Structure 
SNVLAINAGSSSLKFQLIKMPEEQVIAKGLVERIGMPDAIFSVEADGEKDKKVTEIADH
CCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHH
SVAVKMLLESLTSTGVIQSFDEIDAVGHRVVHGGEYFSDSVLITDEIIQQIEEISELAPL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHHHHCCC
HNPANLTGIHAFKEILPEIPMVAVFDTAFHQSMPEASYMYSLPREYYEEYGIRKYGFHGT
CCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCC
SHKYVSERAADLLGVPLSNLRLISCHIGNGASVTAIKDGESIDTSMGFTPLAGVTMGTRS
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCCC
GNIDPALIPYIMDKTGKTADEVLNVLNKESGMLALSGFSSDLRDIEEKSETDARAELALD
CCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHH
VFAGRIHKYIGSYAAKMNGLDAIIFTAGVGENSVTIREKILTGLEFMGIYWDPKLNDVRG
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCC
KEQFINYPHSPVKVIIIPTNEEAMIAKDTVRLSGLNS
HHHHHCCCCCCEEEEEEECCCCCEEEECEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376