Click here to switch to the map view.

The map label for this gene is apt

Identifier: 23099480

GI number: 23099480

Start: 2052095

End: 2052607

Strand: Reverse

Name: apt

Synonym: OB2025

Alternate gene names: 23099480

Gene position: 2052607-2052095 (Counterclockwise)

Preceding gene: 23099481

Following gene: 23099479

Centisome position: 56.54

GC content: 35.48

Gene sequence:

>513_bases
ATGGAATTTAAAAATTATATTGAAATAGTAGAAGATTGGCCTAAGGAAGGCATTAAATTTAAAGATATTACCCCATTGAT
GGCAGATGGAAAAGCTTTTAAATCTGCAGTCGACGAGATTGTAGATTATGCAAATACCAAAGAAATTGATTTAGTAGTAG
GGCCTGAGGCTAGAGGTTTTATTGTTGGTTGTCCGGTTTCTTATGCATTAGAAGTAGGGTTCGCACCTGTAAGAAAAGAA
GGCAAGCTTCCTAGAGAAGTTATTAAAGTAGATTATGGCTTGGAGTATGGAGAAAACGTACTAACGATTCATAAAGATGC
CATAAAACCCGGGCAACGAGTACTTATCACAGATGATTTATTAGCAACTGGGGGAACTATAGAAGCTACGATTAAATTAG
TAGAACAACTTGGCGGTATCGTGGTTGGATGTGCATTTCTTGTTGAATTAGGATATCTAGATGGAAAAGATAAACTAGAA
GGATACGATGTATTAACTTTAATGAAATATTAG

Upstream 100 bases:

>100_bases
GAGATAGAAAAACAACTGTATTATTCAAATTATGAGGATTTAAGAAAATGGTTTATGGAAAGTATGGATTATTTGGAAAA
TCCGAGGGAGGAAGCAACAC

Downstream 100 bases:

>100_bases
AGTTGTAGCGGTAGGTCTGACATTGCCAATAAATGTCAGACCTACTTCTTTATAATTTTATAGTTGGACAGCATGTATCA
AAAAGATTTATAATTAATAT

Product: adenine phosphoribosyltransferase

Products: NA

Alternate protein names: APRT

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MEFKNYIEIVEDWPKEGIKFKDITPLMADGKAFKSAVDEIVDYANTKEIDLVVGPEARGFIVGCPVSYALEVGFAPVRKE
GKLPREVIKVDYGLEYGENVLTIHKDAIKPGQRVLITDDLLATGGTIEATIKLVEQLGGIVVGCAFLVELGYLDGKDKLE
GYDVLTLMKY

Sequences:

>Translated_170_residues
MEFKNYIEIVEDWPKEGIKFKDITPLMADGKAFKSAVDEIVDYANTKEIDLVVGPEARGFIVGCPVSYALEVGFAPVRKE
GKLPREVIKVDYGLEYGENVLTIHKDAIKPGQRVLITDDLLATGGTIEATIKLVEQLGGIVVGCAFLVELGYLDGKDKLE
GYDVLTLMKY
>Mature_170_residues
MEFKNYIEIVEDWPKEGIKFKDITPLMADGKAFKSAVDEIVDYANTKEIDLVVGPEARGFIVGCPVSYALEVGFAPVRKE
GKLPREVIKVDYGLEYGENVLTIHKDAIKPGQRVLITDDLLATGGTIEATIKLVEQLGGIVVGCAFLVELGYLDGKDKLE
GYDVLTLMKY

Specific function: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis

COG id: COG0503

COG function: function code F; Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family

Homologues:

Organism=Homo sapiens, GI4502171, Length=170, Percent_Identity=38.2352941176471, Blast_Score=127, Evalue=7e-30,
Organism=Homo sapiens, GI71773201, Length=124, Percent_Identity=38.7096774193548, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1786675, Length=158, Percent_Identity=54.4303797468354, Blast_Score=168, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17509087, Length=171, Percent_Identity=41.5204678362573, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6323619, Length=172, Percent_Identity=39.5348837209302, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320649, Length=164, Percent_Identity=32.9268292682927, Blast_Score=81, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17136334, Length=161, Percent_Identity=42.8571428571429, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): APT_OCEIH (Q8EPR5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692946.1
- ProteinModelPortal:   Q8EPR5
- SMR:   Q8EPR5
- GeneID:   1018514
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2025
- NMPDR:   fig|221109.1.peg.2023
- HOGENOM:   HBG703830
- OMA:   GILFYDI
- ProtClustDB:   PRK02304
- BioCyc:   OIHE221109:OB2025-MONOMER
- BRENDA:   2.4.2.7
- GO:   GO:0005737
- HAMAP:   MF_00004
- InterPro:   IPR005764
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01090

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.7

Molecular weight: Translated: 18730; Mature: 18730

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKNYIEIVEDWPKEGIKFKDITPLMADGKAFKSAVDEIVDYANTKEIDLVVGPEARGF
CCHHHHHHHHHHHHHCCCEECCCCCCHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCE
IVGCPVSYALEVGFAPVRKEGKLPREVIKVDYGLEYGENVLTIHKDAIKPGQRVLITDDL
EEECCHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCH
LATGGTIEATIKLVEQLGGIVVGCAFLVELGYLDGKDKLEGYDVLTLMKY
HHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MEFKNYIEIVEDWPKEGIKFKDITPLMADGKAFKSAVDEIVDYANTKEIDLVVGPEARGF
CCHHHHHHHHHHHHHCCCEECCCCCCHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCE
IVGCPVSYALEVGFAPVRKEGKLPREVIKVDYGLEYGENVLTIHKDAIKPGQRVLITDDL
EEECCHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCH
LATGGTIEATIKLVEQLGGIVVGCAFLVELGYLDGKDKLEGYDVLTLMKY
HHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376