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The map label for this gene is hisS

Identifier: 23099475

GI number: 23099475

Start: 2045825

End: 2047114

Strand: Reverse

Name: hisS

Synonym: OB2020

Alternate gene names: 23099475

Gene position: 2047114-2045825 (Counterclockwise)

Preceding gene: 23099476

Following gene: 23099474

Centisome position: 56.39

GC content: 38.29

Gene sequence:

>1290_bases
ATGAGTATGAAAGCTCCTAGAGGAACTGTAGATTTATTACCAGAGCAATCAATAAAATGGCAATTTGCGGAAAGTAAAAT
TAAAGAGATATGTCATAATTATCATTATCAAGAAATACGTACCCCATTGTTTGAACATACTGAAGTATTCCAACGTGGAG
TAGGGGATACAACAGATATTGTTCAAAAAGAAATGTATACGTTTGAGGATCGAGGCGGAAGAAGTCTTACATTACGCCCA
GAAGGAACTGCTTCTGTAGCTCGAGCTTATGTTGAGAATAAATTATTTGGAAGTCCTAATCAACCTACCAAATTATTTTA
TTTTGGTTCTATGTTTCGTTATGAACGTCCTCAAAAGGGAAGAATGCGCCAATTGAATCAATTCGGGACAGAGGTTATAG
GCAGTGAAGACCCTGCAGTTGATGCAGAGGTGATGGACTTTGCGATGAATATATATAAAAGCCTAGGTTTAAAATCTGTA
AAACTTGTTATTAATTCGTTAGGTGATCAAGAAAGCAGAGAATCACATCGTAATGCATTAATTCAACACTTTGAACCTCA
CAGGGAAGAATTATGCCATGACTGTCAAAATCGTCTTGATAAAAACCCATTGCGGGTTCTTGATTGTAAGAAAGATAGAG
ATCATCCTGCTATGGGGACTGCACCGAAAATAACTGATTTCTTAAACGAGTACTCGAAGACATATTTCGCTGATGTGATG
ACGTATTTAGATGCATTGGACATTGAATATGTAGTTGATCCCAACCTCGTACGTGGTTTAGATTATTATAACCATACTGC
TTTTGAAATTATGAGTGAAGCAGAAGGATTCGGAGCAATTACTACCCTAGCTGGTGGGGGACGTTATAATGGTTTAGTGG
AAGAGTTTGGAGGTCCTAGCTCACCTGGAATCGGTTTTGGGATGGGCCTAGAAAGGTTAATAATGGCACTTGAGGCTGAA
AATATTACACTTCCTATCGATCAACAACTTGACTGCTTTGTTGCAAATATGGGAGATGAATCTAAGCTGGAAGCAACAAA
AGTCGTGAAGAGACTTCGAGATAACGGAATACAAGCGGATATGGATTACCAGAAGCGTAAAATGAAAGGGCAACTAAAAG
CTGCGGATCGTTATCAGTCTAAATTTGTCATTTTCCTTGGCGATGATGAAATAGAAAAACAGGAAGTCACTTTAAAGGAA
ATGTCTACTGGTGATCAAAGTGAGGTTTCCATGTCTCAACTGGTTGAAGTGCTTCAGGAAAAGCTGAATGGAGGAAAAAT
ATATGAGTGA

Upstream 100 bases:

>100_bases
CATCAACTAGGGTGGCAACGCGGGTAAACTCCCGTCCCTTCTTTAATTTGAAGGGACGGGAGTTTTTTGTATGTACGGAT
AATCATAAGGAGGTAAGTTT

Downstream 100 bases:

>100_bases
AAGATTAATGGCGGGTAGGCTAAACGAAAAAAATATAGGTGAAAAAGTATTGCTTAAAGGTTGGGTTCAAAAGCGTCGAG
ATCTTGGCGGGTTGATTTTT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSMKAPRGTVDLLPEQSIKWQFAESKIKEICHNYHYQEIRTPLFEHTEVFQRGVGDTTDIVQKEMYTFEDRGGRSLTLRP
EGTASVARAYVENKLFGSPNQPTKLFYFGSMFRYERPQKGRMRQLNQFGTEVIGSEDPAVDAEVMDFAMNIYKSLGLKSV
KLVINSLGDQESRESHRNALIQHFEPHREELCHDCQNRLDKNPLRVLDCKKDRDHPAMGTAPKITDFLNEYSKTYFADVM
TYLDALDIEYVVDPNLVRGLDYYNHTAFEIMSEAEGFGAITTLAGGGRYNGLVEEFGGPSSPGIGFGMGLERLIMALEAE
NITLPIDQQLDCFVANMGDESKLEATKVVKRLRDNGIQADMDYQKRKMKGQLKAADRYQSKFVIFLGDDEIEKQEVTLKE
MSTGDQSEVSMSQLVEVLQEKLNGGKIYE

Sequences:

>Translated_429_residues
MSMKAPRGTVDLLPEQSIKWQFAESKIKEICHNYHYQEIRTPLFEHTEVFQRGVGDTTDIVQKEMYTFEDRGGRSLTLRP
EGTASVARAYVENKLFGSPNQPTKLFYFGSMFRYERPQKGRMRQLNQFGTEVIGSEDPAVDAEVMDFAMNIYKSLGLKSV
KLVINSLGDQESRESHRNALIQHFEPHREELCHDCQNRLDKNPLRVLDCKKDRDHPAMGTAPKITDFLNEYSKTYFADVM
TYLDALDIEYVVDPNLVRGLDYYNHTAFEIMSEAEGFGAITTLAGGGRYNGLVEEFGGPSSPGIGFGMGLERLIMALEAE
NITLPIDQQLDCFVANMGDESKLEATKVVKRLRDNGIQADMDYQKRKMKGQLKAADRYQSKFVIFLGDDEIEKQEVTLKE
MSTGDQSEVSMSQLVEVLQEKLNGGKIYE
>Mature_428_residues
SMKAPRGTVDLLPEQSIKWQFAESKIKEICHNYHYQEIRTPLFEHTEVFQRGVGDTTDIVQKEMYTFEDRGGRSLTLRPE
GTASVARAYVENKLFGSPNQPTKLFYFGSMFRYERPQKGRMRQLNQFGTEVIGSEDPAVDAEVMDFAMNIYKSLGLKSVK
LVINSLGDQESRESHRNALIQHFEPHREELCHDCQNRLDKNPLRVLDCKKDRDHPAMGTAPKITDFLNEYSKTYFADVMT
YLDALDIEYVVDPNLVRGLDYYNHTAFEIMSEAEGFGAITTLAGGGRYNGLVEEFGGPSSPGIGFGMGLERLIMALEAEN
ITLPIDQQLDCFVANMGDESKLEATKVVKRLRDNGIQADMDYQKRKMKGQLKAADRYQSKFVIFLGDDEIEKQEVTLKEM
STGDQSEVSMSQLVEVLQEKLNGGKIYE

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=475, Percent_Identity=24.6315789473684, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI6996014, Length=477, Percent_Identity=25.1572327044025, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=40.0943396226415, Blast_Score=343, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI71993693, Length=472, Percent_Identity=24.5762711864407, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71993686, Length=472, Percent_Identity=24.5762711864407, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325290, Length=498, Percent_Identity=24.8995983935743, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24643061, Length=475, Percent_Identity=25.0526315789474, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24643059, Length=494, Percent_Identity=24.0890688259109, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_OCEIH (Q8EPR9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692941.1
- ProteinModelPortal:   Q8EPR9
- SMR:   Q8EPR9
- GeneID:   1018509
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2020
- NMPDR:   fig|221109.1.peg.2019
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   OIHE221109:OB2020-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48739; Mature: 48608

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMKAPRGTVDLLPEQSIKWQFAESKIKEICHNYHYQEIRTPLFEHTEVFQRGVGDTTDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHCCCCHHHH
VQKEMYTFEDRGGRSLTLRPEGTASVARAYVENKLFGSPNQPTKLFYFGSMFRYERPQKG
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHCCCCCCH
RMRQLNQFGTEVIGSEDPAVDAEVMDFAMNIYKSLGLKSVKLVINSLGDQESRESHRNAL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHH
IQHFEPHREELCHDCQNRLDKNPLRVLDCKKDRDHPAMGTAPKITDFLNEYSKTYFADVM
HHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TYLDALDIEYVVDPNLVRGLDYYNHTAFEIMSEAEGFGAITTLAGGGRYNGLVEEFGGPS
HHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCCC
SPGIGFGMGLERLIMALEAENITLPIDQQLDCFVANMGDESKLEATKVVKRLRDNGIQAD
CCCCCHHHHHHHHHHHHHCCCCEECCCCCCHHHEECCCCCHHHHHHHHHHHHHHCCCCCC
MDYQKRKMKGQLKAADRYQSKFVIFLGDDEIEKQEVTLKEMSTGDQSEVSMSQLVEVLQE
CHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KLNGGKIYE
HCCCCCCCC
>Mature Secondary Structure 
SMKAPRGTVDLLPEQSIKWQFAESKIKEICHNYHYQEIRTPLFEHTEVFQRGVGDTTDI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHCCCCHHHH
VQKEMYTFEDRGGRSLTLRPEGTASVARAYVENKLFGSPNQPTKLFYFGSMFRYERPQKG
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHCCCCCCH
RMRQLNQFGTEVIGSEDPAVDAEVMDFAMNIYKSLGLKSVKLVINSLGDQESRESHRNAL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHH
IQHFEPHREELCHDCQNRLDKNPLRVLDCKKDRDHPAMGTAPKITDFLNEYSKTYFADVM
HHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TYLDALDIEYVVDPNLVRGLDYYNHTAFEIMSEAEGFGAITTLAGGGRYNGLVEEFGGPS
HHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCCC
SPGIGFGMGLERLIMALEAENITLPIDQQLDCFVANMGDESKLEATKVVKRLRDNGIQAD
CCCCCHHHHHHHHHHHHHCCCCEECCCCCCHHHEECCCCCHHHHHHHHHHHHHHCCCCCC
MDYQKRKMKGQLKAADRYQSKFVIFLGDDEIEKQEVTLKEMSTGDQSEVSMSQLVEVLQE
CHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KLNGGKIYE
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376