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The map label for this gene is sigG [H]

Identifier: 23098931

GI number: 23098931

Start: 1517130

End: 1517912

Strand: Direct

Name: sigG [H]

Synonym: OB1476

Alternate gene names: 23098931

Gene position: 1517130-1517912 (Clockwise)

Preceding gene: 23098930

Following gene: 23098932

Centisome position: 41.79

GC content: 34.36

Gene sequence:

>783_bases
ATGACAAGACATAAAGTAGAAATATGTGGGGTTGATACATCTAAGCTACCCGTACTTAAAAACGAGGAAATGAAAAAACT
ATTTGTTCGAATGCAAGAAGATGGAGACATTACTGCACGAGAGGAATTAGTGAATGGTAATCTACGTCTCGTGTTGAGTG
TTATACAACGATTTAATAATCGTGGTGAGTATGTGGATGATTTGTTTCAAGTGGGTTGTATAGGTCTTATGAAATCTATT
GATAACTTTGATTTATCACATAATGTACGTTTTTCCACGTATGCTGTTCCGATGATTATTGGTGAAATAAGACGTTATCT
TCGTGATAATAATCCGATTCGTGTTTCTCGTTCTTTAAGAGATATTGCATATAAAGCATTGCAAGTACGTGAGAAATTAA
TTAGTAAAACTTCAAAAGAACCATCTCCTGCAGAAATTGCAAAGGAAATGGATATACCTCAAACAGATGTAGTATTTGCT
TTAGATGCTATACAAGACCCAGTTTCACTCTTTGAACCAATATACAATGATGGGGGAGATCCTATATTTGTTATGGATCA
AATAAGTGATGAGAAAGATAAAGATTCCAGTTGGGTAGACAAATTGTCATTGAAAGAAGGTATGCATCAACTGAATGAGC
GTGAAAAAATGATACTTAATAAACGATTCTTCCAAGGGAAAACACAAATGGAAGTAGCCGATGAAATTGGTATCTCACAA
GCTCAAGTATCTCGTTTAGAAAAAGCGGCAATTAGTCAAATGAATAAGCAAATGTTCGAATAA

Upstream 100 bases:

>100_bases
CTTTTTTTAAAATGTAGGTCCTAATGTTGGTTGCATAAAAAATCCTTCCAGAGGAGATACTGTCCATGAACAGCATCATC
GACTGGGAGGGTTGACTATA

Downstream 100 bases:

>100_bases
ATTGTTACAATGCTATACGGTTGAAACTCCATATGAAAACAATTAGCCTTGAGGTATAAATAAAACTTTAGAGAATTAAT
AACCTGGCCTTATTTTATAA

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: Stage III sporulation protein G [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTRHKVEICGVDTSKLPVLKNEEMKKLFVRMQEDGDITAREELVNGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKSI
DNFDLSHNVRFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVREKLISKTSKEPSPAEIAKEMDIPQTDVVFA
LDAIQDPVSLFEPIYNDGGDPIFVMDQISDEKDKDSSWVDKLSLKEGMHQLNEREKMILNKRFFQGKTQMEVADEIGISQ
AQVSRLEKAAISQMNKQMFE

Sequences:

>Translated_260_residues
MTRHKVEICGVDTSKLPVLKNEEMKKLFVRMQEDGDITAREELVNGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKSI
DNFDLSHNVRFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVREKLISKTSKEPSPAEIAKEMDIPQTDVVFA
LDAIQDPVSLFEPIYNDGGDPIFVMDQISDEKDKDSSWVDKLSLKEGMHQLNEREKMILNKRFFQGKTQMEVADEIGISQ
AQVSRLEKAAISQMNKQMFE
>Mature_259_residues
TRHKVEICGVDTSKLPVLKNEEMKKLFVRMQEDGDITAREELVNGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKSID
NFDLSHNVRFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVREKLISKTSKEPSPAEIAKEMDIPQTDVVFAL
DAIQDPVSLFEPIYNDGGDPIFVMDQISDEKDKDSSWVDKLSLKEGMHQLNEREKMILNKRFFQGKTQMEVADEIGISQA
QVSRLEKAAISQMNKQMFE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes in the forespore [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=255, Percent_Identity=29.8039215686275, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1789448, Length=227, Percent_Identity=29.5154185022026, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789871, Length=258, Percent_Identity=25.968992248062, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29878; Mature: 29747

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRHKVEICGVDTSKLPVLKNEEMKKLFVRMQEDGDITAREELVNGNLRLVLSVIQRFNN
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHCC
RGEYVDDLFQVGCIGLMKSIDNFDLSHNVRFSTYAVPMIIGEIRRYLRDNNPIRVSRSLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEHHHHHH
DIAYKALQVREKLISKTSKEPSPAEIAKEMDIPQTDVVFALDAIQDPVSLFEPIYNDGGD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCC
PIFVMDQISDEKDKDSSWVDKLSLKEGMHQLNEREKMILNKRFFQGKTQMEVADEIGISQ
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH
AQVSRLEKAAISQMNKQMFE
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRHKVEICGVDTSKLPVLKNEEMKKLFVRMQEDGDITAREELVNGNLRLVLSVIQRFNN
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHCC
RGEYVDDLFQVGCIGLMKSIDNFDLSHNVRFSTYAVPMIIGEIRRYLRDNNPIRVSRSLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEHHHHHH
DIAYKALQVREKLISKTSKEPSPAEIAKEMDIPQTDVVFALDAIQDPVSLFEPIYNDGGD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCC
PIFVMDQISDEKDKDSSWVDKLSLKEGMHQLNEREKMILNKRFFQGKTQMEVADEIGISQ
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH
AQVSRLEKAAISQMNKQMFE
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2497052; 2459711; 9384377 [H]