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The map label for this gene is sigE [H]

Identifier: 23098930

GI number: 23098930

Start: 1516288

End: 1517007

Strand: Direct

Name: sigE [H]

Synonym: OB1475

Alternate gene names: 23098930

Gene position: 1516288-1517007 (Clockwise)

Preceding gene: 23098929

Following gene: 23098931

Centisome position: 41.76

GC content: 32.5

Gene sequence:

>720_bases
GTGCGCGTTTGGAAAGTTAAACTAAAATTGCGATGGTATCAGTTGTTAATGAAGTTAGGTCTAAAATCAACAGAAATTTA
TTATATTGGAGGAAGTGAAGCACTACCACCACCACTTTCTAAACAAGAGGAACAAGAACTGTTAATCCGGTTACCTAAAG
GAGACAAGGCAGCCAGGGCGATGTTAATCGAGCGGAATTTGAGATTAGTCGTTTATATAGCAAGAAAATTCGAAAATACT
GGTATAAATATTGAAGATTTAATTAGTATTGGTACGATTGGATTAATAAAGGCAGTCAATACATTTAACCCGGAGAAAAA
AATAAAACTTGCGACGTATGCATCACGTTGTATCGAAAATGAAATATTAATGTACTTACGTAGAAATAATAAACTGAAAT
CAGAAGTGTCATTTGATGAACCATTGAATATTGATTGGGATGGCAATGAATTGCTGTTATCAGATGTAATGGGAACAGAA
GAAGATATAATCACAAAAAATCTAGAATCCACTGTTGACAAAACATTACTAAAGTCGGCATTAGAAAAGCTTAATGCACG
AGAAAAACAAATAATGGAACTGCGCTTTGGACTTATAGGTGAAGAAGAAAAAACACAAAAAGATGTAGCTGATATGTTAG
GAATTTCTCAGTCATATATATCTCGTTTAGAGAAAAAAATAATTCGTCGATTAAAAAAAGAATTTAATAAAATGGTATAG

Upstream 100 bases:

>100_bases
CAATTTGGGAATTTAGTGCGAGACGATAGCTATCACTGCTTATTACATCCACAAATTATGAAACAGTCGATTATACATTC
AGCATAAAGGAGTGAAGGTT

Downstream 100 bases:

>100_bases
CTTGATGTAATAAGGATTTCCCCTTTTTTTAAAATGTAGGTCCTAATGTTGGTTGCATAAAAAATCCTTCCAGAGGAGAT
ACTGTCCATGAACAGCATCA

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: P31; Sigma-29; Stage II sporulation protein GB [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRVWKVKLKLRWYQLLMKLGLKSTEIYYIGGSEALPPPLSKQEEQELLIRLPKGDKAARAMLIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKLKSEVSFDEPLNIDWDGNELLLSDVMGTE
EDIITKNLESTVDKTLLKSALEKLNAREKQIMELRFGLIGEEEKTQKDVADMLGISQSYISRLEKKIIRRLKKEFNKMV

Sequences:

>Translated_239_residues
MRVWKVKLKLRWYQLLMKLGLKSTEIYYIGGSEALPPPLSKQEEQELLIRLPKGDKAARAMLIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKLKSEVSFDEPLNIDWDGNELLLSDVMGTE
EDIITKNLESTVDKTLLKSALEKLNAREKQIMELRFGLIGEEEKTQKDVADMLGISQSYISRLEKKIIRRLKKEFNKMV
>Mature_239_residues
MRVWKVKLKLRWYQLLMKLGLKSTEIYYIGGSEALPPPLSKQEEQELLIRLPKGDKAARAMLIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKLKSEVSFDEPLNIDWDGNELLLSDVMGTE
EDIITKNLESTVDKTLLKSALEKLNAREKQIMELRFGLIGEEEKTQKDVADMLGISQSYISRLEKKIIRRLKKEFNKMV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=269, Percent_Identity=29.7397769516729, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1789871, Length=103, Percent_Identity=43.6893203883495, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27676; Mature: 27676

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVWKVKLKLRWYQLLMKLGLKSTEIYYIGGSEALPPPLSKQEEQELLIRLPKGDKAARA
CCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHEEECCCCCHHHHH
MLIERNLRLVVYIARKFENTGINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIEN
HHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EILMYLRRNNKLKSEVSFDEPLNIDWDGNELLLSDVMGTEEDIITKNLESTVDKTLLKSA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LEKLNAREKQIMELRFGLIGEEEKTQKDVADMLGISQSYISRLEKKIIRRLKKEFNKMV
HHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVWKVKLKLRWYQLLMKLGLKSTEIYYIGGSEALPPPLSKQEEQELLIRLPKGDKAARA
CCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHEEECCCCCHHHHH
MLIERNLRLVVYIARKFENTGINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIEN
HHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EILMYLRRNNKLKSEVSFDEPLNIDWDGNELLLSDVMGTEEDIITKNLESTVDKTLLKSA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LEKLNAREKQIMELRFGLIGEEEKTQKDVADMLGISQSYISRLEKKIIRRLKKEFNKMV
HHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6438529; 9384377; 3104904; 2106671 [H]