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The map label for this gene is dapL

Identifier: 23098858

GI number: 23098858

Start: 1443993

End: 1445108

Strand: Direct

Name: dapL

Synonym: OB1403

Alternate gene names: 23098858

Gene position: 1443993-1445108 (Clockwise)

Preceding gene: 23098857

Following gene: 23098859

Centisome position: 39.77

GC content: 36.2

Gene sequence:

>1116_bases
ATGATGTTACAATTAGAACAAATTCGAAGAGATTTACATCAAATACCAGAATTAGGCTTTCAGGAATTTAAAACGCAAGC
GTATCTATTAGAACGAATTAATGAAATTGCAACAGAAAATGTTGAAATAAAAAAATGGAGTACCGGAATACTTGTATATG
TTCACGGTAAATCCCCAGCTAGAAAAATTGGTTTTCGTGCAGATATAGATGGATTGCCAATTTTAGAGCAAACGAATCTA
CCTTATGCATCCTTGCATGAAGGAAGAATGCATGCTTGTGGGCATGATTTACACATGACGATTGCTCTAGGGGCACTGGA
AAAACTTATTCAAGATCCAATTAATGATGATGTTATCTTTGTATTCCAACCTGCAGAGGAAGGACCCGGTGGAGCAAAGC
CAATGTTAGAATCAGAGGAGTTTCAACAATGGAAACCAGATATGATGTTTGCATTGCATATTGCTCCTGAGCTACCAGTA
GGAACTGTTTCTTCTAAAGCTGGTTTATTATTCGCGAATACAAGCGAATTATTTATCGACTTTGAAGGGGTAGGTGGACA
TGCAGCTTACCCACATTTGACAAAAGATATGACAGTTGCTGCCAGTAATTTTGTAGTTCAATTACAACAAATCGTATCCA
GGGGACTAAATCCACTTGATGGGTCAGTGATTACAATTGGAAAAATGGAAAGTGGATATGTTCAAAATGCTATTGCAGAA
ACAGCTCGCTTAGAAGGAACGATCAGATCTACGGATGCAGATGCAATAGATATGATTAAGAGTAAACTAAATCGGTTAAT
GAAAGGCTTTGAAATTAGCTATGATTGTACTATAAAAGTAGATTATGGTGCTAATTATTACCAAGTAGTAAATGATGCTA
CTTATGTGCAGCAATTTGAAAATGTAATAAATCAAACACAAACCATAACTTATCAACAAGCTGATGCCGCAATGACAGGG
GAAGACTTTGGTGATATGTTAAAAGAAATTCCTGGATTTATGTTTTGGTTAGGCGTGGATTCTTCTTATGGATTGCATAA
TGCACGATTAAACCCTAAGGAAGAAGCAATCGATGTTGGAGTAAATGCTGTAATTTCAATGATTACGTCATTTTGA

Upstream 100 bases:

>100_bases
ACACCAGCAAAAGTATTAAAGGAAATTGATGAACAGACAAAATCTAAAACTGAAATTAAACAAGAGCTTCGTAAATTAGA
TAATTAAGGTTGCTGCAGGT

Downstream 100 bases:

>100_bases
TGATAAAAATACAAACAATTAGTACTGCTAGTAAATGCTAATCGTCAAAATGCCCATCAAACCTATGCTTGATAAACAAC
AAGTGATTATACCTTAATGT

Product: hippurate hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MMLQLEQIRRDLHQIPELGFQEFKTQAYLLERINEIATENVEIKKWSTGILVYVHGKSPARKIGFRADIDGLPILEQTNL
PYASLHEGRMHACGHDLHMTIALGALEKLIQDPINDDVIFVFQPAEEGPGGAKPMLESEEFQQWKPDMMFALHIAPELPV
GTVSSKAGLLFANTSELFIDFEGVGGHAAYPHLTKDMTVAASNFVVQLQQIVSRGLNPLDGSVITIGKMESGYVQNAIAE
TARLEGTIRSTDADAIDMIKSKLNRLMKGFEISYDCTIKVDYGANYYQVVNDATYVQQFENVINQTQTITYQQADAAMTG
EDFGDMLKEIPGFMFWLGVDSSYGLHNARLNPKEEAIDVGVNAVISMITSF

Sequences:

>Translated_371_residues
MMLQLEQIRRDLHQIPELGFQEFKTQAYLLERINEIATENVEIKKWSTGILVYVHGKSPARKIGFRADIDGLPILEQTNL
PYASLHEGRMHACGHDLHMTIALGALEKLIQDPINDDVIFVFQPAEEGPGGAKPMLESEEFQQWKPDMMFALHIAPELPV
GTVSSKAGLLFANTSELFIDFEGVGGHAAYPHLTKDMTVAASNFVVQLQQIVSRGLNPLDGSVITIGKMESGYVQNAIAE
TARLEGTIRSTDADAIDMIKSKLNRLMKGFEISYDCTIKVDYGANYYQVVNDATYVQQFENVINQTQTITYQQADAAMTG
EDFGDMLKEIPGFMFWLGVDSSYGLHNARLNPKEEAIDVGVNAVISMITSF
>Mature_371_residues
MMLQLEQIRRDLHQIPELGFQEFKTQAYLLERINEIATENVEIKKWSTGILVYVHGKSPARKIGFRADIDGLPILEQTNL
PYASLHEGRMHACGHDLHMTIALGALEKLIQDPINDDVIFVFQPAEEGPGGAKPMLESEEFQQWKPDMMFALHIAPELPV
GTVSSKAGLLFANTSELFIDFEGVGGHAAYPHLTKDMTVAASNFVVQLQQIVSRGLNPLDGSVITIGKMESGYVQNAIAE
TARLEGTIRSTDADAIDMIKSKLNRLMKGFEISYDCTIKVDYGANYYQVVNDATYVQQFENVINQTQTITYQQADAAMTG
EDFGDMLKEIPGFMFWLGVDSSYGLHNARLNPKEEAIDVGVNAVISMITSF

Specific function: Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapL subfamily

Homologues:

Organism=Escherichia coli, GI87081880, Length=326, Percent_Identity=26.0736196319018, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPL_OCEIH (Q8ERA3)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692324.1
- HSSP:   P54970
- ProteinModelPortal:   Q8ERA3
- SMR:   Q8ERA3
- GeneID:   1017628
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1403
- NMPDR:   fig|221109.1.peg.1409
- HOGENOM:   HBG708500
- OMA:   THRYLLE
- BioCyc:   OIHE221109:OB1403-MONOMER
- HAMAP:   MF_01692
- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650
- PIRSF:   PIRSF005962
- TIGRFAMs:   TIGR01891

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.47

Molecular weight: Translated: 41215; Mature: 41215

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 68-68 ACT_SITE 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLQLEQIRRDLHQIPELGFQEFKTQAYLLERINEIATENVEIKKWSTGILVYVHGKSPA
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCCH
RKIGFRADIDGLPILEQTNLPYASLHEGRMHACGHDLHMTIALGALEKLIQDPINDDVIF
HHCCCEECCCCCCEEECCCCCHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEE
VFQPAEEGPGGAKPMLESEEFQQWKPDMMFALHIAPELPVGTVSSKAGLLFANTSELFID
EEECCCCCCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEEE
FEGVGGHAAYPHLTKDMTVAASNFVVQLQQIVSRGLNPLDGSVITIGKMESGYVQNAIAE
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHH
TARLEGTIRSTDADAIDMIKSKLNRLMKGFEISYDCTIKVDYGANYYQVVNDATYVQQFE
HHHHCCCEECCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEHHCCHHHHHHHH
NVINQTQTITYQQADAAMTGEDFGDMLKEIPGFMFWLGVDSSYGLHNARLNPKEEAIDVG
HHHHHHHEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHH
VNAVISMITSF
HHHHHHHHHCC
>Mature Secondary Structure
MMLQLEQIRRDLHQIPELGFQEFKTQAYLLERINEIATENVEIKKWSTGILVYVHGKSPA
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCCH
RKIGFRADIDGLPILEQTNLPYASLHEGRMHACGHDLHMTIALGALEKLIQDPINDDVIF
HHCCCEECCCCCCEEECCCCCHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEE
VFQPAEEGPGGAKPMLESEEFQQWKPDMMFALHIAPELPVGTVSSKAGLLFANTSELFID
EEECCCCCCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEEE
FEGVGGHAAYPHLTKDMTVAASNFVVQLQQIVSRGLNPLDGSVITIGKMESGYVQNAIAE
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHH
TARLEGTIRSTDADAIDMIKSKLNRLMKGFEISYDCTIKVDYGANYYQVVNDATYVQQFE
HHHHCCCEECCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEHHCCHHHHHHHH
NVINQTQTITYQQADAAMTGEDFGDMLKEIPGFMFWLGVDSSYGLHNARLNPKEEAIDVG
HHHHHHHEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHH
VNAVISMITSF
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376