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The map label for this gene is dctA [H]

Identifier: 23098737

GI number: 23098737

Start: 1324160

End: 1325392

Strand: Direct

Name: dctA [H]

Synonym: OB1282

Alternate gene names: 23098737

Gene position: 1324160-1325392 (Clockwise)

Preceding gene: 28563925

Following gene: 23098738

Centisome position: 36.47

GC content: 38.36

Gene sequence:

>1233_bases
ATGAAAGAAAAATTCAAAGCATATCGATTTCCTATTATTTTACTGGCATCGATCATTATTGGAAGTATCATAGGGATTGT
ATTTGGTGAAGATGCGACAATTATCAAACCACTTGGGGATTTATTTATTAATTTGTTATTTATGATTGTAATACCATTAG
TTTTCTTCACCATATCATCAGCGATTGCTGCTATGGATAGTATGAAACGTCTTGGTAAAATTATGGGCTCGATGATTACA
GTTTTTGTAGTTACAGGAATAATTGCGTCTGTGTTTATGTTAGTTGCTACAGTTATTTTTGAACCGGGTTCTGGTGTAAA
TATTCCATTAGATACACCAGAAAATGTGGAAGAACAAAACCTATCAGAACAATTGGTTAATACGTTTACAGTACCAGATT
TTGTTGATTTATTCTCCCGTGACAACATGTTAGCGCTCATTGTTTTTTCTGTATTAATTGGGGTAGCGACTGGCCTAACT
GGAGAAAAAGGAAGACCATTCGCTACGTTTTTAACTAGTGCATCTGAAGTATTTATGAGAATCATAAAACTTGTGATGTA
CTATGCTCCAATCGGTCTAGGAGCATACTTTGCAGCACTTGTAGGAGAGTTTGGGGCGGAATTGATAGGTGATTATGCAA
AAGCAATCATCATTTACTACCCGGTTTCGGTCCTATACTTTGCAATTGGATTTACCTTATATGCATTTCTTGCTGGTGGT
AAGAAGGGAGTTACTCGTTTTTGGAAAAATATCTTAGCTCCTGCAGCAACCTCACTTGGTACTGGAAGTAGCATCGCTGC
GCTACCTGTGAACTTACAAGCGGCGAAGCGTATTGGCGTTCCTAAAGATGTTCGCGAAACGGTGCTTCCTTTAGGAGCAA
CGATTCATATGGATGGGTCATGTCTATCGGCAATGCTTAAAATTGCCTTTGTTTTTGGGGTATTTAATATGGATTTCTCC
GGATTTGACACTTTTCTAACTGCTATTGGAATTTGTTTATTGTCTGGTATTGTTATGAGCGGGATTCCAGGCGGAGGGTT
TATGGGAGAGATGATGATCATTACACTGTATGGATTGCCAATGGAAGCTCTACCCATCATAACCGCTATTGGAGTAGTTG
TTGACCCACCTGCAACAATGATTAACGTTACTGGTGACACTGTTTCAAGTATGTTAGTAACCAGAAAAATTGAAGGTAAA
GATTGGATGGAATCAAGCGAAAACCTGACCTAA

Upstream 100 bases:

>100_bases
GAATTATTACTTCATTTTCCTCTTGGACTTCAATACAAAAAGGAGTAGAATATACTAAGTTGGCAAAGTGAAATTACATT
ACATAGTGAAGGTGAGAAAT

Downstream 100 bases:

>100_bases
GAGTAAAAAAATGATTGATAAATCAAATGAGTAAAAATTATCTTTACATTATATTCCATTATTGGTAAGCTTGGGTTGTT
AGTAAATGACATCTAAATAG

Product: H(+):sodium-glutamate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKEKFKAYRFPIILLASIIIGSIIGIVFGEDATIIKPLGDLFINLLFMIVIPLVFFTISSAIAAMDSMKRLGKIMGSMIT
VFVVTGIIASVFMLVATVIFEPGSGVNIPLDTPENVEEQNLSEQLVNTFTVPDFVDLFSRDNMLALIVFSVLIGVATGLT
GEKGRPFATFLTSASEVFMRIIKLVMYYAPIGLGAYFAALVGEFGAELIGDYAKAIIIYYPVSVLYFAIGFTLYAFLAGG
KKGVTRFWKNILAPAATSLGTGSSIAALPVNLQAAKRIGVPKDVRETVLPLGATIHMDGSCLSAMLKIAFVFGVFNMDFS
GFDTFLTAIGICLLSGIVMSGIPGGGFMGEMMIITLYGLPMEALPIITAIGVVVDPPATMINVTGDTVSSMLVTRKIEGK
DWMESSENLT

Sequences:

>Translated_410_residues
MKEKFKAYRFPIILLASIIIGSIIGIVFGEDATIIKPLGDLFINLLFMIVIPLVFFTISSAIAAMDSMKRLGKIMGSMIT
VFVVTGIIASVFMLVATVIFEPGSGVNIPLDTPENVEEQNLSEQLVNTFTVPDFVDLFSRDNMLALIVFSVLIGVATGLT
GEKGRPFATFLTSASEVFMRIIKLVMYYAPIGLGAYFAALVGEFGAELIGDYAKAIIIYYPVSVLYFAIGFTLYAFLAGG
KKGVTRFWKNILAPAATSLGTGSSIAALPVNLQAAKRIGVPKDVRETVLPLGATIHMDGSCLSAMLKIAFVFGVFNMDFS
GFDTFLTAIGICLLSGIVMSGIPGGGFMGEMMIITLYGLPMEALPIITAIGVVVDPPATMINVTGDTVSSMLVTRKIEGK
DWMESSENLT
>Mature_410_residues
MKEKFKAYRFPIILLASIIIGSIIGIVFGEDATIIKPLGDLFINLLFMIVIPLVFFTISSAIAAMDSMKRLGKIMGSMIT
VFVVTGIIASVFMLVATVIFEPGSGVNIPLDTPENVEEQNLSEQLVNTFTVPDFVDLFSRDNMLALIVFSVLIGVATGLT
GEKGRPFATFLTSASEVFMRIIKLVMYYAPIGLGAYFAALVGEFGAELIGDYAKAIIIYYPVSVLYFAIGFTLYAFLAGG
KKGVTRFWKNILAPAATSLGTGSSIAALPVNLQAAKRIGVPKDVRETVLPLGATIHMDGSCLSAMLKIAFVFGVFNMDFS
GFDTFLTAIGICLLSGIVMSGIPGGGFMGEMMIITLYGLPMEALPIITAIGVVVDPPATMINVTGDTVSSMLVTRKIEGK
DWMESSENLT

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI21314632, Length=420, Percent_Identity=26.4285714285714, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI169790839, Length=461, Percent_Identity=25.8134490238612, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI223468564, Length=275, Percent_Identity=28, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI223468566, Length=275, Percent_Identity=28, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4827012, Length=274, Percent_Identity=29.9270072992701, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI40254478, Length=449, Percent_Identity=24.2761692650334, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI5032093, Length=283, Percent_Identity=27.5618374558304, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI66773030, Length=267, Percent_Identity=28.0898876404494, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI194239697, Length=472, Percent_Identity=23.728813559322, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI262359914, Length=389, Percent_Identity=25.9640102827763, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789947, Length=426, Percent_Identity=27.2300469483568, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1790514, Length=383, Percent_Identity=28.7206266318538, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1788024, Length=414, Percent_Identity=24.1545893719807, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71996953, Length=418, Percent_Identity=27.0334928229665, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=407, Percent_Identity=25.0614250614251, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71983106, Length=436, Percent_Identity=24.3119266055046, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71983099, Length=436, Percent_Identity=24.3119266055046, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537407, Length=422, Percent_Identity=24.6445497630332, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI193206654, Length=421, Percent_Identity=25.1781472684086, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17541374, Length=398, Percent_Identity=25.8793969849246, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24583025, Length=443, Percent_Identity=26.410835214447, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17137668, Length=443, Percent_Identity=26.410835214447, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24583023, Length=443, Percent_Identity=26.410835214447, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 43889; Mature: 43889

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHCCCCCHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]