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The map label for this gene is brnQ [H]

Identifier: 23097876

GI number: 23097876

Start: 447525

End: 448877

Strand: Direct

Name: brnQ [H]

Synonym: OB0421

Alternate gene names: 23097876

Gene position: 447525-448877 (Clockwise)

Preceding gene: 23097873

Following gene: 23097880

Centisome position: 12.33

GC content: 36.14

Gene sequence:

>1353_bases
ATGGATAATAAATTACACGTAAGACAATACTTAGCAATTGCTTTTATGCTTTTCGCTTTGTTTTTTGGAGCGGGAAATCT
GATTTTCCCAGCACAACTAGGACAACTAGCAGGAGAAAACATTGTCCCTGCTATTATTGGATTTTTAACAACTGGGGTAG
GCTTACCTTTACTTGGAATATTGGCTATGGGATATTCAGGAAAGAAAAACCTACAAGAGTTAGCGAGCAGGGTACATCCA
ATTTATGGGGTTGGATTTACTGCATTACTTTATCTTACAATTGGACCATTTTTTGCAGCACCAAGAACAGGTACTGTAGC
ATTTGAAGTGGGTATTGGACCGTTTATTAAAGAAGAGTCGTTTCAAACTGGGTTACTCATTTTTACGATTGTATTTTTCC
TAATCACCCTATTATTTTCATTATATCCAGCAAAAATTGTTGATAATATTGGAAAAATCCTGGCACCACTGTTAATTATC
TTATTGGCAGTACTACTGATTGTGTCACTATTCAACCCAATGGGATCCATTCAAGAACCACAAGAAGCGTATCAATCATC
TAGTTTCTTTAAAGGTTTCCTAGAGGGATATAATACAATGGATGCATTGGCAGCACTTGTATTTGGAATCATCGTTATTC
AGTCCATTCGTGCATTAGGAGTAACATCGAAACAGGAAATATTAGTTGCTACAGCGAAAACAGGAATAACTGCTATTATG
TTATTAGGAGTAATCTATGTTGGAATTTCGTATTTAGGAGCAACAAGTGTTGGTACTTTAGGATTTTTTGATAATGGTGG
TCCTGTATTAAGTGGTGCAGCGAGCCATTATTTTGGAACTTTGGGAAGTTTATTAATGGCAGTGCTGATTTCACTGGCCT
GTTTGACTACTGCGATTGGTTTAGTGACAGCAAATGCGGAGTATTTTTATACACTGTTTCCGAAGATTGGATACAAACCT
TTTGTGTTCTTCTTCTCTATATTAACATTTATCATTGCTAATTTTGGATTGACGAATATCATTACATTCTCTATTCCAGT
TTTAATGTTCTTATATCCGTTAGCAATGGTACTAGTTTTATTAACATTTGTATCACCATTGTTCTATCACTCTCGATGGG
TGTATATACCAACTATTGGAGTTACTTTTATACTGAGTATCTTTGATGGATTAAAAGCATTGTGTGATTCTTTAGGAATT
GATAACTTTGCTTGGATGCAACCAATTCTTAATGTGTTTGATACGGTACTTCCATTTTACAATGAAGGTTTAGGATGGGT
AGTACCAGCGCTAGTCGTTACCATCATCATGATGGCCGTTGTTCGCCTTCGCCAGACCAATGCAAATGTATGA

Upstream 100 bases:

>100_bases
TATAGTGGAAGGTGTATGATTATTTCCGTTGTACTTCTAAACTAAAAAATCAGATATTTCAAAGAACTCTTTTTCGGCTA
TTTTGGAGGGAATCACAGCT

Downstream 100 bases:

>100_bases
TAAATGAATATCAAAAACGCCCGGTATCCAATTTGTGGTAACGGGCGTTTTTATTCATGTTTATTTCGATTTTATCTTTT
GAATAGATACTTTGGTCGCC

Product: branched-chain amino acid transporter

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MDNKLHVRQYLAIAFMLFALFFGAGNLIFPAQLGQLAGENIVPAIIGFLTTGVGLPLLGILAMGYSGKKNLQELASRVHP
IYGVGFTALLYLTIGPFFAAPRTGTVAFEVGIGPFIKEESFQTGLLIFTIVFFLITLLFSLYPAKIVDNIGKILAPLLII
LLAVLLIVSLFNPMGSIQEPQEAYQSSSFFKGFLEGYNTMDALAALVFGIIVIQSIRALGVTSKQEILVATAKTGITAIM
LLGVIYVGISYLGATSVGTLGFFDNGGPVLSGAASHYFGTLGSLLMAVLISLACLTTAIGLVTANAEYFYTLFPKIGYKP
FVFFFSILTFIIANFGLTNIITFSIPVLMFLYPLAMVLVLLTFVSPLFYHSRWVYIPTIGVTFILSIFDGLKALCDSLGI
DNFAWMQPILNVFDTVLPFYNEGLGWVVPALVVTIIMMAVVRLRQTNANV

Sequences:

>Translated_450_residues
MDNKLHVRQYLAIAFMLFALFFGAGNLIFPAQLGQLAGENIVPAIIGFLTTGVGLPLLGILAMGYSGKKNLQELASRVHP
IYGVGFTALLYLTIGPFFAAPRTGTVAFEVGIGPFIKEESFQTGLLIFTIVFFLITLLFSLYPAKIVDNIGKILAPLLII
LLAVLLIVSLFNPMGSIQEPQEAYQSSSFFKGFLEGYNTMDALAALVFGIIVIQSIRALGVTSKQEILVATAKTGITAIM
LLGVIYVGISYLGATSVGTLGFFDNGGPVLSGAASHYFGTLGSLLMAVLISLACLTTAIGLVTANAEYFYTLFPKIGYKP
FVFFFSILTFIIANFGLTNIITFSIPVLMFLYPLAMVLVLLTFVSPLFYHSRWVYIPTIGVTFILSIFDGLKALCDSLGI
DNFAWMQPILNVFDTVLPFYNEGLGWVVPALVVTIIMMAVVRLRQTNANV
>Mature_450_residues
MDNKLHVRQYLAIAFMLFALFFGAGNLIFPAQLGQLAGENIVPAIIGFLTTGVGLPLLGILAMGYSGKKNLQELASRVHP
IYGVGFTALLYLTIGPFFAAPRTGTVAFEVGIGPFIKEESFQTGLLIFTIVFFLITLLFSLYPAKIVDNIGKILAPLLII
LLAVLLIVSLFNPMGSIQEPQEAYQSSSFFKGFLEGYNTMDALAALVFGIIVIQSIRALGVTSKQEILVATAKTGITAIM
LLGVIYVGISYLGATSVGTLGFFDNGGPVLSGAASHYFGTLGSLLMAVLISLACLTTAIGLVTANAEYFYTLFPKIGYKP
FVFFFSILTFIIANFGLTNIITFSIPVLMFLYPLAMVLVLLTFVSPLFYHSRWVYIPTIGVTFILSIFDGLKALCDSLGI
DNFAWMQPILNVFDTVLPFYNEGLGWVVPALVVTIIMMAVVRLRQTNANV

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=450, Percent_Identity=37.7777777777778, Blast_Score=294, Evalue=8e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48749; Mature: 48749

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKLHVRQYLAIAFMLFALFFGAGNLIFPAQLGQLAGENIVPAIIGFLTTGVGLPLLGI
CCCCHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LAMGYSGKKNLQELASRVHPIYGVGFTALLYLTIGPFFAAPRTGTVAFEVGIGPFIKEES
HHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
FQTGLLIFTIVFFLITLLFSLYPAKIVDNIGKILAPLLIILLAVLLIVSLFNPMGSIQEP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QEAYQSSSFFKGFLEGYNTMDALAALVFGIIVIQSIRALGVTSKQEILVATAKTGITAIM
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEHHHHHHHHH
LLGVIYVGISYLGATSVGTLGFFDNGGPVLSGAASHYFGTLGSLLMAVLISLACLTTAIG
HHHHHHHHHHHHHHHCCCCCCEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTANAEYFYTLFPKIGYKPFVFFFSILTFIIANFGLTNIITFSIPVLMFLYPLAMVLVL
HHCCCCHHHEEECHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LTFVSPLFYHSRWVYIPTIGVTFILSIFDGLKALCDSLGIDNFAWMQPILNVFDTVLPFY
HHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
NEGLGWVVPALVVTIIMMAVVRLRQTNANV
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDNKLHVRQYLAIAFMLFALFFGAGNLIFPAQLGQLAGENIVPAIIGFLTTGVGLPLLGI
CCCCHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LAMGYSGKKNLQELASRVHPIYGVGFTALLYLTIGPFFAAPRTGTVAFEVGIGPFIKEES
HHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
FQTGLLIFTIVFFLITLLFSLYPAKIVDNIGKILAPLLIILLAVLLIVSLFNPMGSIQEP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QEAYQSSSFFKGFLEGYNTMDALAALVFGIIVIQSIRALGVTSKQEILVATAKTGITAIM
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEHHHHHHHHH
LLGVIYVGISYLGATSVGTLGFFDNGGPVLSGAASHYFGTLGSLLMAVLISLACLTTAIG
HHHHHHHHHHHHHHHCCCCCCEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTANAEYFYTLFPKIGYKPFVFFFSILTFIIANFGLTNIITFSIPVLMFLYPLAMVLVL
HHCCCCHHHEEECHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LTFVSPLFYHSRWVYIPTIGVTFILSIFDGLKALCDSLGIDNFAWMQPILNVFDTVLPFY
HHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
NEGLGWVVPALVVTIIMMAVVRLRQTNANV
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]