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The map label for this gene is glgB

Identifier: 23097861

GI number: 23097861

Start: 432481

End: 434394

Strand: Direct

Name: glgB

Synonym: OB0406

Alternate gene names: 23097861

Gene position: 432481-434394 (Clockwise)

Preceding gene: 23097858

Following gene: 23097862

Centisome position: 11.91

GC content: 37.3

Gene sequence:

>1914_bases
TTGTATCATATATCCGCAGAAGACATCTACTTATTCCATCAAGGTACAAACTTTGAGAGTCATCAATTTTTAGGATGCCA
CGAGATAAACTGGAAAGGAAAACGAGGGTATCGATTTGCTGTTTGGGCACCTAATGCGTTAAAAGTTTGCGTGGTAGGTG
ATTTTAACAATTGGGAAGAAAATAGCCATCCTTTAGAGAAGTTTACGGATGAAGGTCTTTGGGGTGGTTTTATAGCAGAT
ATTCCACCAGCAACATCTTATAAGTATCATATATGTTCATCTGAAGACACTTCAATATTAAAAGCGGATCCATTCGCAAC
GCAAGCAGAGCGAAGGCCTAAAACTGCATCTGTAATCCCCGCAGCAAATGGTTACCAATGGAGTGATGATCAATGGATCG
AACAAAGAAATACTTATGATCCATATTCATCTCCAATTTCCATTTACGAAGTTCATTTAGGTACATGGAAAAAAACACTC
AAAAAACAATTTTTATCCTACCGAGAATTAGCAACACAACTAATACCATACGTAAAATCCCTAGGTTACACTCATATAGA
ATTATTACCCATAAATGAACATCCATTCGACCGATCTTGGGGATATCAAATCACAGGATATTTTGCGGTTACATCAAGAT
ATGGAAACCCATCGGACTTTAAATATTTTATTGATCAATGTCATCAACATCAAATAGGTGTCATCTTAGATTGGGTCCCT
GGACATTTTTGTAAAGATGATTTCGGACTGCGGCAATTTGACGGAGCACCACTGTATGAATACAGGGATCCGAAAAAATC
CGAAAAAAAGTCATGGGGTACATTAGCGTTTGATTATGGCAGACCTGAAGTTCAAAGCTTTCTTATTTCCAATGCAATTT
ATTGGTTGAAAGAGTTCCATATTGATGGACTTCGTGTCGATGCAGTAGCGAGCATGTTGTATTTGAACTTTGATCGATAT
GATGAGGAAGAAAAAATATACAATACATACGGTGGGGAGGAGAATCTAGAGGCCTTTGCCTTTTTGAGAAAGCTAAATAA
AGTCGTTTTTTCTTATATACCAGGTGCCTTAATGATGGCAGAAGATAGTTCGGATTTACCTTTAGTAACAGCTCCTGTAA
ATAAAGGTGGACTAGGGTTTAATTATAAATGGAACATGGGATGGATGAATGATTTGCTTTCTTTTATGGAGAAGGAATCG
ATACATCGGAAGTGGCATCATAACCGGCTGACTTTCTCGTTTATGTACACCTATTCGGAAAACTATTTATTGCCACTCTC
TCATGATGAGGTCGTACACGGCAAGAAATCACTTTTAGATAAAATGCCTGGAGATCAATGGCAGCAATTTGCAAACTTAC
GTCTCCTCTATGGATATATGTATACACATCCTGGCAAGAAGCTAGTATTTATGGGGGGAGAATTAGCGCAATATGCGGAA
TGGAAGGACACGGAGGAGTTGGACTGGCATTTACTAGAATATCCGCTCCACAAAGGTATTTATCACTATATTAAAAATTT
GAATGAATTATATCAGCAACATCCTGAACTTTATGAATTGGATCACCTAAGTGAAGGATTTGAGTGGATAGACCCACATA
ATATTGATCAAAGTGTAATAGCATTTAGAAGGAAAGCGAATAAACCAAATCAAGAATTAATTATTATTTGTAATTTCACA
CCTCAAGTTCATTTTGACTATAAGATTGGCGTTCCTGAATCTGGAAGATATAAAGAAATTTTTAATTCTGATTCAGTAAG
GTTTGGAGGGTCTGGTCAAATAAATGAAGGGGAACATTTTAGCTTTCCGGAGAAATGGCATGGTTTATCACAGCATATAA
AGATTAAAGTGCCACCATTAGCCATATCCGTTTTTCAAATCGAAGTTGAAAGGGAGAGATTGCCGCGTGAGTGA

Upstream 100 bases:

>100_bases
GAATATTCTGACTATTTTCTTGAACTAGTATTATTTTATCGCTATAATGTTAGGGAATACAAGATTTTCTTTTTCAATTG
TTCAAAGGGGGCAGCGTTGG

Downstream 100 bases:

>100_bases
ATGCATCGGAATGTTATTAGCGGGAGGAGAAGGAAAGCGACTGGGCATGTTAACGAAACAAGGAGCAAAGCCCGCTGTTC
CGTTTGGAGGTAAGTATCGT

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MYHISAEDIYLFHQGTNFESHQFLGCHEINWKGKRGYRFAVWAPNALKVCVVGDFNNWEENSHPLEKFTDEGLWGGFIAD
IPPATSYKYHICSSEDTSILKADPFATQAERRPKTASVIPAANGYQWSDDQWIEQRNTYDPYSSPISIYEVHLGTWKKTL
KKQFLSYRELATQLIPYVKSLGYTHIELLPINEHPFDRSWGYQITGYFAVTSRYGNPSDFKYFIDQCHQHQIGVILDWVP
GHFCKDDFGLRQFDGAPLYEYRDPKKSEKKSWGTLAFDYGRPEVQSFLISNAIYWLKEFHIDGLRVDAVASMLYLNFDRY
DEEEKIYNTYGGEENLEAFAFLRKLNKVVFSYIPGALMMAEDSSDLPLVTAPVNKGGLGFNYKWNMGWMNDLLSFMEKES
IHRKWHHNRLTFSFMYTYSENYLLPLSHDEVVHGKKSLLDKMPGDQWQQFANLRLLYGYMYTHPGKKLVFMGGELAQYAE
WKDTEELDWHLLEYPLHKGIYHYIKNLNELYQQHPELYELDHLSEGFEWIDPHNIDQSVIAFRRKANKPNQELIIICNFT
PQVHFDYKIGVPESGRYKEIFNSDSVRFGGSGQINEGEHFSFPEKWHGLSQHIKIKVPPLAISVFQIEVERERLPRE

Sequences:

>Translated_637_residues
MYHISAEDIYLFHQGTNFESHQFLGCHEINWKGKRGYRFAVWAPNALKVCVVGDFNNWEENSHPLEKFTDEGLWGGFIAD
IPPATSYKYHICSSEDTSILKADPFATQAERRPKTASVIPAANGYQWSDDQWIEQRNTYDPYSSPISIYEVHLGTWKKTL
KKQFLSYRELATQLIPYVKSLGYTHIELLPINEHPFDRSWGYQITGYFAVTSRYGNPSDFKYFIDQCHQHQIGVILDWVP
GHFCKDDFGLRQFDGAPLYEYRDPKKSEKKSWGTLAFDYGRPEVQSFLISNAIYWLKEFHIDGLRVDAVASMLYLNFDRY
DEEEKIYNTYGGEENLEAFAFLRKLNKVVFSYIPGALMMAEDSSDLPLVTAPVNKGGLGFNYKWNMGWMNDLLSFMEKES
IHRKWHHNRLTFSFMYTYSENYLLPLSHDEVVHGKKSLLDKMPGDQWQQFANLRLLYGYMYTHPGKKLVFMGGELAQYAE
WKDTEELDWHLLEYPLHKGIYHYIKNLNELYQQHPELYELDHLSEGFEWIDPHNIDQSVIAFRRKANKPNQELIIICNFT
PQVHFDYKIGVPESGRYKEIFNSDSVRFGGSGQINEGEHFSFPEKWHGLSQHIKIKVPPLAISVFQIEVERERLPRE
>Mature_637_residues
MYHISAEDIYLFHQGTNFESHQFLGCHEINWKGKRGYRFAVWAPNALKVCVVGDFNNWEENSHPLEKFTDEGLWGGFIAD
IPPATSYKYHICSSEDTSILKADPFATQAERRPKTASVIPAANGYQWSDDQWIEQRNTYDPYSSPISIYEVHLGTWKKTL
KKQFLSYRELATQLIPYVKSLGYTHIELLPINEHPFDRSWGYQITGYFAVTSRYGNPSDFKYFIDQCHQHQIGVILDWVP
GHFCKDDFGLRQFDGAPLYEYRDPKKSEKKSWGTLAFDYGRPEVQSFLISNAIYWLKEFHIDGLRVDAVASMLYLNFDRY
DEEEKIYNTYGGEENLEAFAFLRKLNKVVFSYIPGALMMAEDSSDLPLVTAPVNKGGLGFNYKWNMGWMNDLLSFMEKES
IHRKWHHNRLTFSFMYTYSENYLLPLSHDEVVHGKKSLLDKMPGDQWQQFANLRLLYGYMYTHPGKKLVFMGGELAQYAE
WKDTEELDWHLLEYPLHKGIYHYIKNLNELYQQHPELYELDHLSEGFEWIDPHNIDQSVIAFRRKANKPNQELIIICNFT
PQVHFDYKIGVPESGRYKEIFNSDSVRFGGSGQINEGEHFSFPEKWHGLSQHIKIKVPPLAISVFQIEVERERLPRE

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=628, Percent_Identity=26.4331210191083, Blast_Score=197, Evalue=3e-50,
Organism=Escherichia coli, GI1789839, Length=630, Percent_Identity=47.6190476190476, Blast_Score=603, Evalue=1e-173,
Organism=Escherichia coli, GI2367229, Length=330, Percent_Identity=23.030303030303, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554896, Length=606, Percent_Identity=28.0528052805281, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI32564391, Length=592, Percent_Identity=27.1959459459459, Blast_Score=180, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320826, Length=645, Percent_Identity=28.062015503876, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI28573410, Length=626, Percent_Identity=29.073482428115, Blast_Score=222, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_OCEIH (Q8CZE8)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691327.1
- ProteinModelPortal:   Q8CZE8
- GeneID:   1015693
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0406
- NMPDR:   fig|221109.1.peg.412
- HOGENOM:   HBG287139
- OMA:   PPLATIM
- ProtClustDB:   PRK05402
- BioCyc:   OIHE221109:OB0406-MONOMER
- BRENDA:   2.4.1.18
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat

EC number: =2.4.1.18

Molecular weight: Translated: 74652; Mature: 74652

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: ACT_SITE 202-202 ACT_SITE 237-237 ACT_SITE 242-242 ACT_SITE 305-305 ACT_SITE 307-307 ACT_SITE 361-361 ACT_SITE 428-428 ACT_SITE 429-429

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYHISAEDIYLFHQGTNFESHQFLGCHEINWKGKRGYRFAVWAPNALKVCVVGDFNNWEE
CEEECCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCC
NSHPLEKFTDEGLWGGFIADIPPATSYKYHICSSEDTSILKADPFATQAERRPKTASVIP
CCCCHHHHCCCCCCCCEECCCCCCCCEEEEEECCCCCCEEECCCCCHHHHCCCCCCEEEE
AANGYQWSDDQWIEQRNTYDPYSSPISIYEVHLGTWKKTLKKQFLSYRELATQLIPYVKS
CCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGYTHIELLPINEHPFDRSWGYQITGYFAVTSRYGNPSDFKYFIDQCHQHQIGVILDWVP
CCCCEEEEEECCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCC
GHFCKDDFGLRQFDGAPLYEYRDPKKSEKKSWGTLAFDYGRPEVQSFLISNAIYWLKEFH
CCCCCCCCCCEECCCCCCCCCCCCCCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHC
IDGLRVDAVASMLYLNFDRYDEEEKIYNTYGGEENLEAFAFLRKLNKVVFSYIPGALMMA
CCCEEHHHHHHHHHEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
EDSSDLPLVTAPVNKGGLGFNYKWNMGWMNDLLSFMEKESIHRKWHHNRLTFSFMYTYSE
CCCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECC
NYLLPLSHDEVVHGKKSLLDKMPGDQWQQFANLRLLYGYMYTHPGKKLVFMGGELAQYAE
CEEEECCCCHHHCCHHHHHHCCCCHHHHHHHCEEEEEEEEEECCCCEEEEECCCHHHHHC
WKDTEELDWHLLEYPLHKGIYHYIKNLNELYQQHPELYELDHLSEGFEWIDPHNIDQSVI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHH
AFRRKANKPNQELIIICNFTPQVHFDYKIGVPESGRYKEIFNSDSVRFGGSGQINEGEHF
HHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHCCCCEEECCCCCCCCCCCC
SFPEKWHGLSQHIKIKVPPLAISVFQIEVERERLPRE
CCCHHHCCCCCEEEEEECCEEEEEEEEEEEHHCCCCC
>Mature Secondary Structure
MYHISAEDIYLFHQGTNFESHQFLGCHEINWKGKRGYRFAVWAPNALKVCVVGDFNNWEE
CEEECCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCC
NSHPLEKFTDEGLWGGFIADIPPATSYKYHICSSEDTSILKADPFATQAERRPKTASVIP
CCCCHHHHCCCCCCCCEECCCCCCCCEEEEEECCCCCCEEECCCCCHHHHCCCCCCEEEE
AANGYQWSDDQWIEQRNTYDPYSSPISIYEVHLGTWKKTLKKQFLSYRELATQLIPYVKS
CCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGYTHIELLPINEHPFDRSWGYQITGYFAVTSRYGNPSDFKYFIDQCHQHQIGVILDWVP
CCCCEEEEEECCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCC
GHFCKDDFGLRQFDGAPLYEYRDPKKSEKKSWGTLAFDYGRPEVQSFLISNAIYWLKEFH
CCCCCCCCCCEECCCCCCCCCCCCCCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHC
IDGLRVDAVASMLYLNFDRYDEEEKIYNTYGGEENLEAFAFLRKLNKVVFSYIPGALMMA
CCCEEHHHHHHHHHEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
EDSSDLPLVTAPVNKGGLGFNYKWNMGWMNDLLSFMEKESIHRKWHHNRLTFSFMYTYSE
CCCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECC
NYLLPLSHDEVVHGKKSLLDKMPGDQWQQFANLRLLYGYMYTHPGKKLVFMGGELAQYAE
CEEEECCCCHHHCCHHHHHHCCCCHHHHHHHCEEEEEEEEEECCCCEEEEECCCHHHHHC
WKDTEELDWHLLEYPLHKGIYHYIKNLNELYQQHPELYELDHLSEGFEWIDPHNIDQSVI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHH
AFRRKANKPNQELIIICNFTPQVHFDYKIGVPESGRYKEIFNSDSVRFGGSGQINEGEHF
HHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHCCCCEEECCCCCCCCCCCC
SFPEKWHGLSQHIKIKVPPLAISVFQIEVERERLPRE
CCCHHHCCCCCEEEEEECCEEEEEEEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376