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The map label for this gene is ywnH [H]

Identifier: 23097789

GI number: 23097789

Start: 357076

End: 357582

Strand: Direct

Name: ywnH [H]

Synonym: OB0334

Alternate gene names: 23097789

Gene position: 357076-357582 (Clockwise)

Preceding gene: 23097788

Following gene: 23097790

Centisome position: 9.84

GC content: 34.71

Gene sequence:

>507_bases
ATGAGTAAAAATTTAACTTTTAGAAACGCCACTATAGAAGATTTACCAGAGATAGTAGCTATTTATAATTCAACGATCGT
CAGTAGAATGGTAACAGCTGATACAGAACCGGTTACTGTAGAAGAGAGGCAAAATTGGTTTAATGAACATGACTCTGTCA
GAAGGCCTTTATTAGTAGCAGAATTAGAAGGAGAAATCTGTGGGTGGATTAGTTTAGAATCCTTCTATGGACGTCCCGCT
TATAATCAAACTGCAGAAGTTAGTATATATATAGATGAACGTTTTAGAGGAGAAGGACTAGGAAAGAAATTATTAAACTA
TATCATAGAAAAAAGTCCTTCTTATGGTATCGAAATATTATTGGGATTCATATTTGCCCACAATGAGCCAAGCATTAAAT
TATTCGAACGCTTCGGGTTTGAACAATGGGCCCATCTACCGAGCGTTGCTAAACTAGATAGCGTGAATAGGGATTTAATT
ATACTAGGGAAAAATATAAGTAAATAA

Upstream 100 bases:

>100_bases
TAGAAACAACGTGGCATTCATTCAAAATAGATCAATTCTGAATGAAATAAAGTAAAAATATAAATAAGAAAAAAACTAAA
ATATAAGGTAGGTCAACAGC

Downstream 100 bases:

>100_bases
GTAAAAAGAAGGATTAACTTGTGTAATCCTTTTTTAATGGAAAAATATACCTAAAATTCACTTTACAAGTCGTGTTATTT
GTTTTAATATTAATATTCTG

Product: phosphinothricin N-acetyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MSKNLTFRNATIEDLPEIVAIYNSTIVSRMVTADTEPVTVEERQNWFNEHDSVRRPLLVAELEGEICGWISLESFYGRPA
YNQTAEVSIYIDERFRGEGLGKKLLNYIIEKSPSYGIEILLGFIFAHNEPSIKLFERFGFEQWAHLPSVAKLDSVNRDLI
ILGKNISK

Sequences:

>Translated_168_residues
MSKNLTFRNATIEDLPEIVAIYNSTIVSRMVTADTEPVTVEERQNWFNEHDSVRRPLLVAELEGEICGWISLESFYGRPA
YNQTAEVSIYIDERFRGEGLGKKLLNYIIEKSPSYGIEILLGFIFAHNEPSIKLFERFGFEQWAHLPSVAKLDSVNRDLI
ILGKNISK
>Mature_167_residues
SKNLTFRNATIEDLPEIVAIYNSTIVSRMVTADTEPVTVEERQNWFNEHDSVRRPLLVAELEGEICGWISLESFYGRPAY
NQTAEVSIYIDERFRGEGLGKKLLNYIIEKSPSYGIEILLGFIFAHNEPSIKLFERFGFEQWAHLPSVAKLDSVNRDLII
LGKNISK

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=146, Percent_Identity=27.3972602739726, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 19240; Mature: 19109

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNLTFRNATIEDLPEIVAIYNSTIVSRMVTADTEPVTVEERQNWFNEHDSVRRPLLVA
CCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCEEEE
ELEGEICGWISLESFYGRPAYNQTAEVSIYIDERFRGEGLGKKLLNYIIEKSPSYGIEIL
ECCCCEEEEEEEHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHH
LGFIFAHNEPSIKLFERFGFEQWAHLPSVAKLDSVNRDLIILGKNISK
HHHHHHCCCCCHHHHHHCCHHHHHCCCCHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure 
SKNLTFRNATIEDLPEIVAIYNSTIVSRMVTADTEPVTVEERQNWFNEHDSVRRPLLVA
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCEEEE
ELEGEICGWISLESFYGRPAYNQTAEVSIYIDERFRGEGLGKKLLNYIIEKSPSYGIEIL
ECCCCEEEEEEEHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHH
LGFIFAHNEPSIKLFERFGFEQWAHLPSVAKLDSVNRDLIILGKNISK
HHHHHHCCCCCHHHHHHCCHHHHHCCCCHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]