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The map label for this gene is gntT [H]

Identifier: 22127746

GI number: 22127746

Start: 4316445

End: 4317761

Strand: Reverse

Name: gntT [H]

Synonym: y3875

Alternate gene names: 22127746

Gene position: 4317761-4316445 (Counterclockwise)

Preceding gene: 22127750

Following gene: 22127742

Centisome position: 93.85

GC content: 51.18

Gene sequence:

>1317_bases
ATGCCATTAATCATCGTCGCAGTTGGCGTTGCCATGCTGCTATTACTGATGATCCGTTACAAACTTAACGGATTTATCTC
GCTGATACTGGTCGCGTTAGCGGTAGGTATCATGCAAGGGATGCCAATCGATAAAGTTGTCGGCTCGATCAAAGCGGGTG
TCGGCGGAACATTAGGTAGCCTGGCCTTGATTATGGGCTTCGGGGCCATGCTCGGTAAATTGCTGGCCGATTGTGGCGGC
GCTCAACGTATCGCGACCACCTTGATTGAGAAATTTGGTCAAAAACATATTCAATGGGCGGTGGTACTGACCGGCTTTAC
TGTGGGTTTTGCCCTGTTTTATGAAGTGGGTTTTGTGCTGTTATTGCCACTGGTATTCAGCATTGCGGCCTCTGCCCGCA
TCCCCTTGCTATATGTGGGTGTTCCCATGGCGGCGGCGCTTTCTGTCACTCATGGTTTTCTGCCACCTCACCCAGGCCCG
ACCGCCATTGCCACCATTTTCAACGCCGATATGGGCAAAACGCTGCTGTACGGCACGTTGCTGGCAATCCCAACCGTGAT
TTTGGCAGGCCCGGTGTACGCCCGCTTTCTAAAAGGAATTGATAAGCCTGTTCCTGAAGGGTTGTATAACCCGAAAATCT
TTACCGATAAAGAGATGCCAAGCTTTGGGGTCAGTGTCAGTACGGCGCTGGTACCGGTTATTTTGATGGCATTGCGGGCC
GTAGCAGAGATGATTTTGCCTAAAGGCCATTCGCTGCTCCCCTATGCCGAGTTCTTTGGTGATCCTGTAATTGCTACGCT
GATTGCCGTGTTAATTGCAATTTTCACCTTTGGTCTAAACCGGGGCCGCTCAGTGGAATCGGTCATGGAGACCCTGTCTG
ATTCCATCAAGATCATCGCAATGATGCTGTTAGTTATTGGAGGGGGCGGTGCTTTCAAGCAGGTTTTAGTCGATAGCGGC
ATAGAGCAATATATTGCTGGCATGATGGGCAATAGCGGGGCATCACCTATTCTGATGGCATGGTCCATCGCCGCCTTACT
GCGTATCGCCCTGGGTTCTGCGACGGTGGCAGCGATCACCGCTGGGGGCATTGTCGCACCGTTAATCGCGACCACCGGTG
CCAGCCCTGAGTTGATGGTCATTGCCGTTGGTTCCGGTAGCGTGATCTTCTCTCACGTGAATGACCCTGGCTTCTGGCTA
TTCAAGGAGTATTTCAACCTAACCATTATGGAAACCATTAAATCGTGGTCAGTACTGGAAACCATTATTTCAGTCTGCGG
GTTGATCGGCTGTTTGTTGCTGTCTCTGGTTATTTAG

Upstream 100 bases:

>100_bases
ATAGCGCCGTTATCACAATAGATAAAACAGCTATCACGACAGATAAAAAACACTGCCTTCGTACTCAATCACGCGAGCCA
GGAAAGGCCGGAGAAATATT

Downstream 100 bases:

>100_bases
CCAACGGCGCGATAAATAGTCGTTATATAAATGGCTGTTATATAAATGGTTATTATGTAAATAGTTACTATATAAATGAT
TGCTATATTTTTTATATACT

Product: high-affinity H+/gluconate symporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: Gluconate permease; Gnt-I system [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPLIIVAVGVAMLLLLMIRYKLNGFISLILVALAVGIMQGMPIDKVVGSIKAGVGGTLGSLALIMGFGAMLGKLLADCGG
AQRIATTLIEKFGQKHIQWAVVLTGFTVGFALFYEVGFVLLLPLVFSIAASARIPLLYVGVPMAAALSVTHGFLPPHPGP
TAIATIFNADMGKTLLYGTLLAIPTVILAGPVYARFLKGIDKPVPEGLYNPKIFTDKEMPSFGVSVSTALVPVILMALRA
VAEMILPKGHSLLPYAEFFGDPVIATLIAVLIAIFTFGLNRGRSVESVMETLSDSIKIIAMMLLVIGGGGAFKQVLVDSG
IEQYIAGMMGNSGASPILMAWSIAALLRIALGSATVAAITAGGIVAPLIATTGASPELMVIAVGSGSVIFSHVNDPGFWL
FKEYFNLTIMETIKSWSVLETIISVCGLIGCLLLSLVI

Sequences:

>Translated_438_residues
MPLIIVAVGVAMLLLLMIRYKLNGFISLILVALAVGIMQGMPIDKVVGSIKAGVGGTLGSLALIMGFGAMLGKLLADCGG
AQRIATTLIEKFGQKHIQWAVVLTGFTVGFALFYEVGFVLLLPLVFSIAASARIPLLYVGVPMAAALSVTHGFLPPHPGP
TAIATIFNADMGKTLLYGTLLAIPTVILAGPVYARFLKGIDKPVPEGLYNPKIFTDKEMPSFGVSVSTALVPVILMALRA
VAEMILPKGHSLLPYAEFFGDPVIATLIAVLIAIFTFGLNRGRSVESVMETLSDSIKIIAMMLLVIGGGGAFKQVLVDSG
IEQYIAGMMGNSGASPILMAWSIAALLRIALGSATVAAITAGGIVAPLIATTGASPELMVIAVGSGSVIFSHVNDPGFWL
FKEYFNLTIMETIKSWSVLETIISVCGLIGCLLLSLVI
>Mature_437_residues
PLIIVAVGVAMLLLLMIRYKLNGFISLILVALAVGIMQGMPIDKVVGSIKAGVGGTLGSLALIMGFGAMLGKLLADCGGA
QRIATTLIEKFGQKHIQWAVVLTGFTVGFALFYEVGFVLLLPLVFSIAASARIPLLYVGVPMAAALSVTHGFLPPHPGPT
AIATIFNADMGKTLLYGTLLAIPTVILAGPVYARFLKGIDKPVPEGLYNPKIFTDKEMPSFGVSVSTALVPVILMALRAV
AEMILPKGHSLLPYAEFFGDPVIATLIAVLIAIFTFGLNRGRSVESVMETLSDSIKIIAMMLLVIGGGGAFKQVLVDSGI
EQYIAGMMGNSGASPILMAWSIAALLRIALGSATVAAITAGGIVAPLIATTGASPELMVIAVGSGSVIFSHVNDPGFWLF
KEYFNLTIMETIKSWSVLETIISVCGLIGCLLLSLVI

Specific function: Part of the gluconate utilization system Gnt-I; high- affinity intake of gluconate [H]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994935, Length=438, Percent_Identity=85.3881278538813, Blast_Score=737, Evalue=0.0,
Organism=Escherichia coli, GI1790716, Length=438, Percent_Identity=61.1872146118721, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI2367370, Length=449, Percent_Identity=51.2249443207127, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1790776, Length=438, Percent_Identity=40.1826484018265, Blast_Score=345, Evalue=4e-96,
Organism=Escherichia coli, GI1788707, Length=435, Percent_Identity=37.0114942528736, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI48994939, Length=434, Percent_Identity=36.8663594470046, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1789097, Length=442, Percent_Identity=33.710407239819, Blast_Score=218, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 45791; Mature: 45660

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLIIVAVGVAMLLLLMIRYKLNGFISLILVALAVGIMQGMPIDKVVGSIKAGVGGTLGS
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
LALIMGFGAMLGKLLADCGGAQRIATTLIEKFGQKHIQWAVVLTGFTVGFALFYEVGFVL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLPLVFSIAASARIPLLYVGVPMAAALSVTHGFLPPHPGPTAIATIFNADMGKTLLYGTL
HHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
LAIPTVILAGPVYARFLKGIDKPVPEGLYNPKIFTDKEMPSFGVSVSTALVPVILMALRA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAEMILPKGHSLLPYAEFFGDPVIATLIAVLIAIFTFGLNRGRSVESVMETLSDSIKIIA
HHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MMLLVIGGGGAFKQVLVDSGIEQYIAGMMGNSGASPILMAWSIAALLRIALGSATVAAIT
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AGGIVAPLIATTGASPELMVIAVGSGSVIFSHVNDPGFWLFKEYFNLTIMETIKSWSVLE
HCHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
TIISVCGLIGCLLLSLVI
HHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PLIIVAVGVAMLLLLMIRYKLNGFISLILVALAVGIMQGMPIDKVVGSIKAGVGGTLGS
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
LALIMGFGAMLGKLLADCGGAQRIATTLIEKFGQKHIQWAVVLTGFTVGFALFYEVGFVL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLPLVFSIAASARIPLLYVGVPMAAALSVTHGFLPPHPGPTAIATIFNADMGKTLLYGTL
HHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
LAIPTVILAGPVYARFLKGIDKPVPEGLYNPKIFTDKEMPSFGVSVSTALVPVILMALRA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAEMILPKGHSLLPYAEFFGDPVIATLIAVLIAIFTFGLNRGRSVESVMETLSDSIKIIA
HHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MMLLVIGGGGAFKQVLVDSGIEQYIAGMMGNSGASPILMAWSIAALLRIALGSATVAAIT
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AGGIVAPLIATTGASPELMVIAVGSGSVIFSHVNDPGFWLFKEYFNLTIMETIKSWSVLE
HCHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
TIISVCGLIGCLLLSLVI
HHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 2845225; 7984428; 3040894; 9045817 [H]