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The map label for this gene is hmsT [H]

Identifier: 22127627

GI number: 22127627

Start: 4173979

End: 4175151

Strand: Direct

Name: hmsT [H]

Synonym: y3756

Alternate gene names: 22127627

Gene position: 4173979-4175151 (Clockwise)

Preceding gene: 22127624

Following gene: 22127629

Centisome position: 90.72

GC content: 42.11

Gene sequence:

>1173_bases
ATGCAGAGTAAATTGAATATGAATAGCCACTCCTACGATCAGTTTTTGAAAAGCAAGCATCGACTCTCTTTACTGCTTTT
CCTATTTCTTAATGCATCATCAGCTATTTTCAGTATGCTATCATCGTCGCCCAAGACACCCGCGGTAACGTTCCCTGTGG
TGATGATTGCGGTTATAAGCTTGAGCGCGGCGGCTTATCTAATTTTTATTTCAAAGAACGACACGGGCAGAAATAACTAT
GTTGATAAAAATAACTATGTTGATAAAAGTAACCATGTTGACAAAAATAACTATGCGAATAAATTGAATTTATTTTCAAT
CCTCATCGGTCTACTTTGGGCATGGCAAATCATTCTTAAATATGAATATCTAGAGACTAACGAAGATAATTACTTATTAC
TCAGTCTGTTTACGATCTTTTTTATCAGTACCATTGCTTTGTCTGATAATTTTCTCGCGTTTTGCCTGCATACGGCACCT
TCGGCAATAACCGTCATCTTACTGGATGATTTTCATAATATTTTCCGCATTATATTTACCATCATGTTACCGCTGATTGG
TTTTTCTTTGCATCACTTGATGTTGCGTCGCACTGATAATTTCACCCGTAAGCTGGTTGATCACCTTTCCAACGAAAGAG
AAAAATTCAGTGATTTAAGCATGATAGACCCTCTGACCAGTTTGTATAATCGCCGTGGGCTGGAGAGCAAGCTTGAAACG
TTGTTATCTCAGTCGCCGGGAAATCATTATGTCCTGCTGCTCGATATCGATCACTTTAAAGCCTATAACGACAACTATGG
CCACACCATGGGCGATCAGGCTCTGGTACGGATTTCGGCGGCCATTCGCGATGCTGTGCGTTCAAGAGATATCGTGGTGC
GTTATGGTGGTGAAGAGTTTCTCGTACTCCTAACACATGTCAGTGAAGAGTACGCCAACCAATTGGCAGAGCGTGTACGT
CAGCGTGTTTTAGGCCTGGATATTCCACATGTCTTTAATCATAAGGTCTCTACGACGGTCACCTTAAGTGCGGGTATTTC
CCCCCTGCAAGCCTATGACTTGGCCGCGTCATTGAAAGCCGCGGATGAAGCGCTCTACCGGGCCAAGAGAAATGGCCGCA
ATAACATCGAATTTGCTAAAGATGAATTATCAAATGTACAGTCTTCCCCTTGA

Upstream 100 bases:

>100_bases
ATTTAAACAATAATAATTCCCAACAAAATTTATTTGTCGTGATGTCGGAATAAATACCTCACGAGGATATGATTTATTAG
TCTACTGACAGCACGATATT

Downstream 100 bases:

>100_bases
TTAACTCACTGAACATACGGACGCTCTATGCCAGTTATTATTTTTAATGATTGATTATCAAGTTAAATACTGCACGTTTA
ATTTCACTTCCATTCCGTTA

Product: HmsT protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MQSKLNMNSHSYDQFLKSKHRLSLLLFLFLNASSAIFSMLSSSPKTPAVTFPVVMIAVISLSAAAYLIFISKNDTGRNNY
VDKNNYVDKSNHVDKNNYANKLNLFSILIGLLWAWQIILKYEYLETNEDNYLLLSLFTIFFISTIALSDNFLAFCLHTAP
SAITVILLDDFHNIFRIIFTIMLPLIGFSLHHLMLRRTDNFTRKLVDHLSNEREKFSDLSMIDPLTSLYNRRGLESKLET
LLSQSPGNHYVLLLDIDHFKAYNDNYGHTMGDQALVRISAAIRDAVRSRDIVVRYGGEEFLVLLTHVSEEYANQLAERVR
QRVLGLDIPHVFNHKVSTTVTLSAGISPLQAYDLAASLKAADEALYRAKRNGRNNIEFAKDELSNVQSSP

Sequences:

>Translated_390_residues
MQSKLNMNSHSYDQFLKSKHRLSLLLFLFLNASSAIFSMLSSSPKTPAVTFPVVMIAVISLSAAAYLIFISKNDTGRNNY
VDKNNYVDKSNHVDKNNYANKLNLFSILIGLLWAWQIILKYEYLETNEDNYLLLSLFTIFFISTIALSDNFLAFCLHTAP
SAITVILLDDFHNIFRIIFTIMLPLIGFSLHHLMLRRTDNFTRKLVDHLSNEREKFSDLSMIDPLTSLYNRRGLESKLET
LLSQSPGNHYVLLLDIDHFKAYNDNYGHTMGDQALVRISAAIRDAVRSRDIVVRYGGEEFLVLLTHVSEEYANQLAERVR
QRVLGLDIPHVFNHKVSTTVTLSAGISPLQAYDLAASLKAADEALYRAKRNGRNNIEFAKDELSNVQSSP
>Mature_390_residues
MQSKLNMNSHSYDQFLKSKHRLSLLLFLFLNASSAIFSMLSSSPKTPAVTFPVVMIAVISLSAAAYLIFISKNDTGRNNY
VDKNNYVDKSNHVDKNNYANKLNLFSILIGLLWAWQIILKYEYLETNEDNYLLLSLFTIFFISTIALSDNFLAFCLHTAP
SAITVILLDDFHNIFRIIFTIMLPLIGFSLHHLMLRRTDNFTRKLVDHLSNEREKFSDLSMIDPLTSLYNRRGLESKLET
LLSQSPGNHYVLLLDIDHFKAYNDNYGHTMGDQALVRISAAIRDAVRSRDIVVRYGGEEFLVLLTHVSEEYANQLAERVR
QRVLGLDIPHVFNHKVSTTVTLSAGISPLQAYDLAASLKAADEALYRAKRNGRNNIEFAKDELSNVQSSP

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=225, Percent_Identity=36.4444444444444, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787262, Length=176, Percent_Identity=36.3636363636364, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1786584, Length=176, Percent_Identity=33.5227272727273, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=35.3293413173653, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=40.1197604790419, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788085, Length=180, Percent_Identity=32.2222222222222, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=35.2201257861635, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081974, Length=234, Percent_Identity=29.0598290598291, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787541, Length=165, Percent_Identity=32.7272727272727, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=33.7349397590361, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788381, Length=159, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788956, Length=151, Percent_Identity=33.7748344370861, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081977, Length=173, Percent_Identity=29.4797687861272, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44321; Mature: 44321

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSKLNMNSHSYDQFLKSKHRLSLLLFLFLNASSAIFSMLSSSPKTPAVTFPVVMIAVIS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LSAAAYLIFISKNDTGRNNYVDKNNYVDKSNHVDKNNYANKLNLFSILIGLLWAWQIILK
HCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YEYLETNEDNYLLLSLFTIFFISTIALSDNFLAFCLHTAPSAITVILLDDFHNIFRIIFT
HHHEECCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
IMLPLIGFSLHHLMLRRTDNFTRKLVDHLSNEREKFSDLSMIDPLTSLYNRRGLESKLET
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLSQSPGNHYVLLLDIDHFKAYNDNYGHTMGDQALVRISAAIRDAVRSRDIVVRYGGEEF
HHHCCCCCEEEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEE
LVLLTHVSEEYANQLAERVRQRVLGLDIPHVFNHKVSTTVTLSAGISPLQAYDLAASLKA
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
ADEALYRAKRNGRNNIEFAKDELSNVQSSP
HHHHHHHHHHCCCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure
MQSKLNMNSHSYDQFLKSKHRLSLLLFLFLNASSAIFSMLSSSPKTPAVTFPVVMIAVIS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LSAAAYLIFISKNDTGRNNYVDKNNYVDKSNHVDKNNYANKLNLFSILIGLLWAWQIILK
HCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YEYLETNEDNYLLLSLFTIFFISTIALSDNFLAFCLHTAPSAITVILLDDFHNIFRIIFT
HHHEECCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
IMLPLIGFSLHHLMLRRTDNFTRKLVDHLSNEREKFSDLSMIDPLTSLYNRRGLESKLET
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLSQSPGNHYVLLLDIDHFKAYNDNYGHTMGDQALVRISAAIRDAVRSRDIVVRYGGEEF
HHHCCCCCEEEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEE
LVLLTHVSEEYANQLAERVRQRVLGLDIPHVFNHKVSTTVTLSAGISPLQAYDLAASLKA
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
ADEALYRAKRNGRNNIEFAKDELSNVQSSP
HHHHHHHHHHCCCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]