Click here to switch to the map view.

The map label for this gene is holD [H]

Identifier: 22127623

GI number: 22127623

Start: 4171292

End: 4171729

Strand: Reverse

Name: holD [H]

Synonym: y3752

Alternate gene names: 22127623

Gene position: 4171729-4171292 (Counterclockwise)

Preceding gene: 22127625

Following gene: 161484738

Centisome position: 90.67

GC content: 53.2

Gene sequence:

>438_bases
ATGGCATCAAGACGAGACTTGCTGTTACAGCAGCTTGGCATTACGCAGTGGGTATTGCGCCGTCCTGCCGTCTTGCAGGG
TGAGATTGCAATACATTTACCTGAAGATACCCGCCTACTGATTGTGGCACAGCAACTGCCTGAACACGGTGATCCACTGT
TGTGCGATGTGCTACGCAGTTTGGGCCTAACCCCCCATCAGGTTTATGCCTTGACGCCGGATCAGGTCGCGATGTTGCCG
GAAGATACGCAGTGCAACAGTTGGCGATTGGGCATCAGTGAGCCGCTTGCGGTGGCCGGTGCACAATTACATAGCCCAGA
ATTAGCTGGGCTTTATCAAGATGCGGGCGCTAAGCGCGCACTTTGGCAGCAGATTTGTCATTATGAAGCAGATTTCTATC
CTGACGCCAGCCGATCTGGCCACAGCATACCAAATTGA

Upstream 100 bases:

>100_bases
CCTTAATAATATAATCGGGGATTATAGAGGTTTGTTGGCGCATGTTCGATGGGTTTGCTAGCATAGCGCCAAATTACGGC
TTGCTCTGACAGGAAATAGC

Downstream 100 bases:

>100_bases
ACAAGCCAGCCATGCGTTCCCATGGACGGAAAAAACGTTCGCCAGTAATCAGGGCGACCGGTACCTCAATTTTAAGTTGA
GCATCGGCCAGGAAATGGCG

Product: DNA polymerase III subunit psi

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MASRRDLLLQQLGITQWVLRRPAVLQGEIAIHLPEDTRLLIVAQQLPEHGDPLLCDVLRSLGLTPHQVYALTPDQVAMLP
EDTQCNSWRLGISEPLAVAGAQLHSPELAGLYQDAGAKRALWQQICHYEADFYPDASRSGHSIPN

Sequences:

>Translated_145_residues
MASRRDLLLQQLGITQWVLRRPAVLQGEIAIHLPEDTRLLIVAQQLPEHGDPLLCDVLRSLGLTPHQVYALTPDQVAMLP
EDTQCNSWRLGISEPLAVAGAQLHSPELAGLYQDAGAKRALWQQICHYEADFYPDASRSGHSIPN
>Mature_144_residues
ASRRDLLLQQLGITQWVLRRPAVLQGEIAIHLPEDTRLLIVAQQLPEHGDPLLCDVLRSLGLTPHQVYALTPDQVAMLPE
DTQCNSWRLGISEPLAVAGAQLHSPELAGLYQDAGAKRALWQQICHYEADFYPDASRSGHSIPN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The exact function of the psi subunit is unknown [H]

COG id: COG3050

COG function: function code L; DNA polymerase III, psi subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790831, Length=134, Percent_Identity=59.7014925373134, Blast_Score=165, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004615
- InterPro:   IPR018382
- ProDom:   PD032475 [H]

Pfam domain/function: PF03603 DNA_III_psi [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 16078; Mature: 15946

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRRDLLLQQLGITQWVLRRPAVLQGEIAIHLPEDTRLLIVAQQLPEHGDPLLCDVLRS
CCCHHHHHHHHHCHHHHHHHCCCEEECCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHH
LGLTPHQVYALTPDQVAMLPEDTQCNSWRLGISEPLAVAGAQLHSPELAGLYQDAGAKRA
CCCCCCEEEEECCCCEEECCCCCCCCCEECCCCCCHHHHCCCCCCCHHHHHHHCCCHHHH
LWQQICHYEADFYPDASRSGHSIPN
HHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASRRDLLLQQLGITQWVLRRPAVLQGEIAIHLPEDTRLLIVAQQLPEHGDPLLCDVLRS
CCHHHHHHHHHCHHHHHHHCCCEEECCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHH
LGLTPHQVYALTPDQVAMLPEDTQCNSWRLGISEPLAVAGAQLHSPELAGLYQDAGAKRA
CCCCCCEEEEECCCCEEECCCCCCCCCEECCCCCCHHHHCCCCCCCHHHHHHHCCCHHHH
LWQQICHYEADFYPDASRSGHSIPN
HHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8389364; 8366044; 2828880; 7610040; 9278503; 8505305; 1575709 [H]