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The map label for this gene is proP [H]

Identifier: 22127583

GI number: 22127583

Start: 4125055

End: 4126443

Strand: Reverse

Name: proP [H]

Synonym: y3709

Alternate gene names: 22127583

Gene position: 4126443-4125055 (Counterclockwise)

Preceding gene: 22127584

Following gene: 22127580

Centisome position: 89.69

GC content: 46.65

Gene sequence:

>1389_bases
ATGTCTCAAGATCACAATTCATCTCAAGTCGGCTCATCCCAAATCGACTCATCTCAACATCATGTTGATCAACAACATAA
TCATAATCGTCGATTGAATAAGCAAGATTACAAAACACTCACCTTAGCCGCATTAGGTGGAGCGTTAGAGTTCTATGATT
TTATTATTTTTGTCTTCTTTGCCGCGGTTATTGGTGATCTTTTCTTCCCGGCTGACATGCCTGAATGGCTTCGTCAGGTG
CAGACGTTCGGTATTTTTGCTGCGGGTTACTTGGCTCGCCCATTGGGGGGGATCATCATGGCACATTTTGGTGATTTGGT
TGGGCGTAAAAAGATGTTTACTCTTAGCATCTTATTAATGGCGTTGCCAACACTGGCTATTGGCATGTTGCCGACATATG
CCACTATCGGGATCACCGCACCTTTACTTCTACTATTAATGCGGGTATTACAAGGTGCTGCCATTGGTGGCGAAGTTCCC
GGTGCATGGGTCTTTGTGGCAGAACATGTGCCACGTAAACGTATTGGCATTGCCTGCGGTACCTTAACGGCCGGTCTGAC
TGCTGGGATTTTGTTAGGATCGCTGGTTGCTACAGTCATGAATACTACGCTGGGCCATCAGGCGATTTTGGAGGGGGGAT
GGCGTATACCGTTCTTCTTGGGTGGAATTTTCGGTTTGTTTGCCATGTACTTACGCCGTTGGTTGCAGGAAACTCCGATC
TTTAAAGAAATGCAGGCGCGTAAAACATTGGCTGAAGAATTGCCGCTGAAATCGGTGGTGGTAAACCATAAGAAAGAAGT
GGTTGTTTCGATGCTGCTGACTTGGTTGCTCTCTGCTGGCATCGTCGTCGTTATTTTGATGACACCAACCTATCTACAGA
AGCAGTTTAATGTACCGCCAGAGTTGGCATTGCAGGCAAACAGTTTGGCGATTATCGCATTGGTTATCGGCTGTGTGGTT
GCCGGATTGGCAATTGACCGCTTTGGGGCCAGTAAAACCTTTATCGTTGGCAGCCTGATGCTGGCGATGTCGACATGGTC
GTTTTATCACACCAATCTGACTAATCCATCTCAGTTATTTCCCCACTATATGTTGGCTGGCTTCTGTGTCGGTATTGTTG
GTGCAGTGCCTTATGTCATGGTGCGTGCCTTCCCGGCAGAAGTCCGCTTCACGGGCATCTCTTTCTCGTACAATGTGGCC
TATGCCATTTTTGGTGGTTTAACACCTATTCTGGTGACCTTATGGATGAAGTCATCTGCCATGGCTCCGGCGTATTACAT
GCTGGTGCTATCGCTGGTGGGATTGTTATTGGGTATTTATCTGCGTAACGACATTAATAGTGAAGTGAAAGTTCAGATGC
CTAAAAGGGTGATGAACGGAGTTAATTAA

Upstream 100 bases:

>100_bases
CATTATGTGCGTGTGGGGAATTTTGTACGGTATAGTCATGTTAACTTTACAAAGAGTAGAAAAATTTTTCTTCCCCAACT
CTGGCTTATAACGGTGTCAT

Downstream 100 bases:

>100_bases
GTCAATTCACTTTAAAAAGCCCATAATTATTATCAATAAATTATGGGCTTTTATCTTTTTCTCTATACCTTAGAATTCTG
ATTTAAAGGTAAATCCGACT

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFFAAVIGDLFFPADMPEWLRQV
QTFGIFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGMLPTYATIGITAPLLLLLMRVLQGAAIGGEVP
GAWVFVAEHVPRKRIGIACGTLTAGLTAGILLGSLVATVMNTTLGHQAILEGGWRIPFFLGGIFGLFAMYLRRWLQETPI
FKEMQARKTLAEELPLKSVVVNHKKEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFNVPPELALQANSLAIIALVIGCVV
AGLAIDRFGASKTFIVGSLMLAMSTWSFYHTNLTNPSQLFPHYMLAGFCVGIVGAVPYVMVRAFPAEVRFTGISFSYNVA
YAIFGGLTPILVTLWMKSSAMAPAYYMLVLSLVGLLLGIYLRNDINSEVKVQMPKRVMNGVN

Sequences:

>Translated_462_residues
MSQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFFAAVIGDLFFPADMPEWLRQV
QTFGIFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGMLPTYATIGITAPLLLLLMRVLQGAAIGGEVP
GAWVFVAEHVPRKRIGIACGTLTAGLTAGILLGSLVATVMNTTLGHQAILEGGWRIPFFLGGIFGLFAMYLRRWLQETPI
FKEMQARKTLAEELPLKSVVVNHKKEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFNVPPELALQANSLAIIALVIGCVV
AGLAIDRFGASKTFIVGSLMLAMSTWSFYHTNLTNPSQLFPHYMLAGFCVGIVGAVPYVMVRAFPAEVRFTGISFSYNVA
YAIFGGLTPILVTLWMKSSAMAPAYYMLVLSLVGLLLGIYLRNDINSEVKVQMPKRVMNGVN
>Mature_461_residues
SQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFFAAVIGDLFFPADMPEWLRQVQ
TFGIFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGMLPTYATIGITAPLLLLLMRVLQGAAIGGEVPG
AWVFVAEHVPRKRIGIACGTLTAGLTAGILLGSLVATVMNTTLGHQAILEGGWRIPFFLGGIFGLFAMYLRRWLQETPIF
KEMQARKTLAEELPLKSVVVNHKKEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFNVPPELALQANSLAIIALVIGCVVA
GLAIDRFGASKTFIVGSLMLAMSTWSFYHTNLTNPSQLFPHYMLAGFCVGIVGAVPYVMVRAFPAEVRFTGISFSYNVAY
AIFGGLTPILVTLWMKSSAMAPAYYMLVLSLVGLLLGIYLRNDINSEVKVQMPKRVMNGVN

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=433, Percent_Identity=32.5635103926097, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI1788292, Length=398, Percent_Identity=31.9095477386935, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789941, Length=416, Percent_Identity=30.2884615384615, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788942, Length=429, Percent_Identity=31.2354312354312, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082231, Length=478, Percent_Identity=24.2677824267782, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87082404, Length=392, Percent_Identity=22.4489795918367, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 50505; Mature: 50374

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHCCCCC
>Mature Secondary Structure 
SQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFF
CCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH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CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]