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The map label for this gene is setA [H]

Identifier: 22127523

GI number: 22127523

Start: 4047170

End: 4048351

Strand: Reverse

Name: setA [H]

Synonym: y3649

Alternate gene names: 22127523

Gene position: 4048351-4047170 (Counterclockwise)

Preceding gene: 22127528

Following gene: 161484747

Centisome position: 87.99

GC content: 49.49

Gene sequence:

>1182_bases
ATGAGATCTGCACGAGCCTTTTCCCGTCGTATCAACCCGGTTTTCCTCGCTTTCTTTGTGGTGGCTTTTCTTTCCGGCAT
CGCGGGTGCGTTGCAAGCCCCGACATTAAGCCTGTTTTTAAGCACAGAGTTAAAAGTGCGTCCGCTGTGGGTCGGATTGT
TTTATACCGTGAATGCCATTGCCGGTATCACCGTGAGCTTCCTACTGGCCAAACGCTCAGATCTGGGCGGGGATCGGCGT
AAATTAATTCTGATCTGCTATCTGATGGCGGTCGGAAATTGCCTGCTATTCGCTTTTAATCGTGATTATCTGACGTTGAT
CACTTTAGGTGTTTTGATGGCCTCGGTGGCGAATACCGCGATGCCGCAAATTTTCGCCTTGGCACGGGAATATGCGGATA
ACTCTGCGCGTGAAGTGGTGATGTTCAGTTCGATTATGCGTGCGCAATTATCATTGGCTTGGGTTATCGGGCCACCGCTG
TCATTTATGTTAGCGCTGAATTATGGCTTTACGCTGATGTTTAGCATTGCCGCCGGTATTTTTGTTATTAGTGCGCTGGT
GGTCTGGTTTATCCTGCCGTCGGTTAAACAGCCTGAGCCCGTAGCCGATGCCCCCGTGGTGGTACAAGGCAGTTTGTTTG
CCGATAAAAATGTCTTACTGCTGTTCATTGCCTCAATGCTGATGTGGACGTGTAATACCATGTACATCATTGATATGCCG
CTCTATATCACCGCTAATTTAGGGTTGCCTGAGCGTTTGGCGGGTTTGCTGATGGGGACCGCCGCCGGGCTGGAAATTCC
TATCATGTTGTTGGCAGGTTACTCGGTTAGGCGCTTTGGTAAACGTAAAATCATGCTGTTTGCGGTGATGGCGGGGGTGC
TGTTTTATACCGGGCTGGTGCTGTTTAAATTCAAAACCGCACTGATGATATTGCAGATTTTTAATGCCATCTTTATCGGC
ATCGTCGCCGGTATTGGTATGCTCTATTTTCAGGATCTCATGCCGGGGCGGGCAGGTGCCGCAACCACGTTATTTACCAA
CAGTATTTCTAGTGGGGTGATTTTGGCCGGTGTGCTGCAAGGTGTATTGACTGAAACCTGGGGGCATAATGCGGTTTATG
TCGCTGCGATGGTGTTGGTTATTTTGGCGTTGATCATCTGCGCCAAAGTGAGAGAGGCTTAG

Upstream 100 bases:

>100_bases
ATAAAAGCCTATATCACTTCTTTATGTATAAGAGCTTCTGTAGCATAGGGCACCATTATGATTATGCTAATAATAGCTTT
TTCTGGCGCTGGAGCCTGGA

Downstream 100 bases:

>100_bases
GTGAGAGCGGTTTAGGTCAGCGCAGCTTAGCGGTTTGCCCCTGCTTTGTGCCAGAAGCGGCGGGGGTCAGCTGGCGGTAG
GATGAGTTAAACGTGTTACT

Product: efflux protein

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MRSARAFSRRINPVFLAFFVVAFLSGIAGALQAPTLSLFLSTELKVRPLWVGLFYTVNAIAGITVSFLLAKRSDLGGDRR
KLILICYLMAVGNCLLFAFNRDYLTLITLGVLMASVANTAMPQIFALAREYADNSAREVVMFSSIMRAQLSLAWVIGPPL
SFMLALNYGFTLMFSIAAGIFVISALVVWFILPSVKQPEPVADAPVVVQGSLFADKNVLLLFIASMLMWTCNTMYIIDMP
LYITANLGLPERLAGLLMGTAAGLEIPIMLLAGYSVRRFGKRKIMLFAVMAGVLFYTGLVLFKFKTALMILQIFNAIFIG
IVAGIGMLYFQDLMPGRAGAATTLFTNSISSGVILAGVLQGVLTETWGHNAVYVAAMVLVILALIICAKVREA

Sequences:

>Translated_393_residues
MRSARAFSRRINPVFLAFFVVAFLSGIAGALQAPTLSLFLSTELKVRPLWVGLFYTVNAIAGITVSFLLAKRSDLGGDRR
KLILICYLMAVGNCLLFAFNRDYLTLITLGVLMASVANTAMPQIFALAREYADNSAREVVMFSSIMRAQLSLAWVIGPPL
SFMLALNYGFTLMFSIAAGIFVISALVVWFILPSVKQPEPVADAPVVVQGSLFADKNVLLLFIASMLMWTCNTMYIIDMP
LYITANLGLPERLAGLLMGTAAGLEIPIMLLAGYSVRRFGKRKIMLFAVMAGVLFYTGLVLFKFKTALMILQIFNAIFIG
IVAGIGMLYFQDLMPGRAGAATTLFTNSISSGVILAGVLQGVLTETWGHNAVYVAAMVLVILALIICAKVREA
>Mature_393_residues
MRSARAFSRRINPVFLAFFVVAFLSGIAGALQAPTLSLFLSTELKVRPLWVGLFYTVNAIAGITVSFLLAKRSDLGGDRR
KLILICYLMAVGNCLLFAFNRDYLTLITLGVLMASVANTAMPQIFALAREYADNSAREVVMFSSIMRAQLSLAWVIGPPL
SFMLALNYGFTLMFSIAAGIFVISALVVWFILPSVKQPEPVADAPVVVQGSLFADKNVLLLFIASMLMWTCNTMYIIDMP
LYITANLGLPERLAGLLMGTAAGLEIPIMLLAGYSVRRFGKRKIMLFAVMAGVLFYTGLVLFKFKTALMILQIFNAIFIG
IVAGIGMLYFQDLMPGRAGAATTLFTNSISSGVILAGVLQGVLTETWGHNAVYVAAMVLVILALIICAKVREA

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport IPTG, lactose and glucose. Has broad substrate specificity, with preferences for glucosides or galactosides with alkyl or

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI48994875, Length=385, Percent_Identity=70.9090909090909, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1790091, Length=391, Percent_Identity=48.3375959079284, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1788495, Length=376, Percent_Identity=51.8617021276596, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42695; Mature: 42695

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSARAFSRRINPVFLAFFVVAFLSGIAGALQAPTLSLFLSTELKVRPLWVGLFYTVNAI
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEHHHHHHHHHHHH
AGITVSFLLAKRSDLGGDRRKLILICYLMAVGNCLLFAFNRDYLTLITLGVLMASVANTA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHH
MPQIFALAREYADNSAREVVMFSSIMRAQLSLAWVIGPPLSFMLALNYGFTLMFSIAAGI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHH
FVISALVVWFILPSVKQPEPVADAPVVVQGSLFADKNVLLLFIASMLMWTCNTMYIIDMP
HHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEC
LYITANLGLPERLAGLLMGTAAGLEIPIMLLAGYSVRRFGKRKIMLFAVMAGVLFYTGLV
EEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
LFKFKTALMILQIFNAIFIGIVAGIGMLYFQDLMPGRAGAATTLFTNSISSGVILAGVLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHH
GVLTETWGHNAVYVAAMVLVILALIICAKVREA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSARAFSRRINPVFLAFFVVAFLSGIAGALQAPTLSLFLSTELKVRPLWVGLFYTVNAI
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEHHHHHHHHHHHH
AGITVSFLLAKRSDLGGDRRKLILICYLMAVGNCLLFAFNRDYLTLITLGVLMASVANTA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHH
MPQIFALAREYADNSAREVVMFSSIMRAQLSLAWVIGPPLSFMLALNYGFTLMFSIAAGI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHH
FVISALVVWFILPSVKQPEPVADAPVVVQGSLFADKNVLLLFIASMLMWTCNTMYIIDMP
HHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEC
LYITANLGLPERLAGLLMGTAAGLEIPIMLLAGYSVRRFGKRKIMLFAVMAGVLFYTGLV
EEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
LFKFKTALMILQIFNAIFIGIVAGIGMLYFQDLMPGRAGAATTLFTNSISSGVILAGVLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHH
GVLTETWGHNAVYVAAMVLVILALIICAKVREA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 10209755; 10438463 [H]