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The map label for this gene is mdtN [H]

Identifier: 22127432

GI number: 22127432

Start: 3937672

End: 3938721

Strand: Direct

Name: mdtN [H]

Synonym: y3558

Alternate gene names: 22127432

Gene position: 3937672-3938721 (Clockwise)

Preceding gene: 22127431

Following gene: 22127433

Centisome position: 85.59

GC content: 54.0

Gene sequence:

>1050_bases
ATGAATAAACAACAAGGGAGTGGTCGTAAGCGGCAACTGGCGTTAATTGTCGCCGGGGTCATCATTATTGCCGCCGCGAT
TTCAGGGTGGCTTTCAGTGCGGCAGACCACACTGAACCCGCTGTCTGAGGATGCGGAGTTGGGGGCGAGTGTAGTGCATA
TTGCCAGCTCGGTTCCTGGCCGTATCATTTCGATCAATGTCGAGGAAAATAGCAAAGTCCGGCGTGGTGACTTACTGTTT
TCCATTGAGCCAGATCTCTACCGTCTGCAAGTCGAACAAGCGCAGGCTGAGTTGAAAATGGCAGAGGCTACCCACGATAC
CCAACAACGGACAGTGGTCGCGGAACGATCCAACGCCGCGATCACCAATGAGCAGATTGTACGGGCGCAAGCCAATCTCA
AATTAGCCACTCAGACATTGGCGCGGTTACAACCTTTGCGCCCTAAAGGCTATGTCACCGCCCAGCAGGTAGATGATGCA
GCAACAGCGAAACACGATGCGGAAGTGAGTCTTAAACAGGCATTAAAACAGTCAGTGGCCGCTGAAGCCTTGGTCAGCAG
CACGGCGTCTTCTGAAGCGCTGGTGGTAGCGCGCCGTGCAGCCCTTGCCATTGCCGAACGGGAACTGGCTAATACGCAAA
TACACGCGCCTAATGATGGTCGGGTCGTCGGGTTGACTGTTTCCGCAGGGGAATTTGTCGCCCCGGATCAGGCTATTTTC
ACCCTTATCAATACCGAACATTGGCATGCTTCGGCATTTTTCCGTGAAACAGAGTTGAAACATATCAAGGTGGGTGATTG
TGCCACCGTGTATGTCATGGCAGATAGGCAGCGGGCGATTCAAGGACGGGTCGAAGGGATTGGTTGGGGGGTTAGCTCCG
AGGATATGCTAAATATTCCGCGCGGCCTGCCTTATGTGCCGAAGTCATTAAACTGGGTGCGTGTTGTTCAGCGTTTCCCG
GTGAGGATCAGCCTGGAAAAACCGCCGGAGGACCTGATGCGTATCGGGGCGACTGCTGTGGTTATCGTGCGAAATGACGA
CGGTTGCTGA

Upstream 100 bases:

>100_bases
TGATGAGGTGCTGCCATGGCGGCTGTTGGTGTATGTCTGCCTGGCGCTTGCTGTGGCATTCATCTGTTCCCTATTACTGT
TTACGCGATGAATAATTCAT

Downstream 100 bases:

>100_bases
CGGTACGCTTGGCGGCATAATCCGTCAGGCGAAAGCTGACTTAGCCTTTTTCCCAGGGCGGGTGGCGATGGCGTGGCGTG
TTGCCGTGCTATGTGCATTG

Product: multidrug resistance protein MdtN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MNKQQGSGRKRQLALIVAGVIIIAAAISGWLSVRQTTLNPLSEDAELGASVVHIASSVPGRIISINVEENSKVRRGDLLF
SIEPDLYRLQVEQAQAELKMAEATHDTQQRTVVAERSNAAITNEQIVRAQANLKLATQTLARLQPLRPKGYVTAQQVDDA
ATAKHDAEVSLKQALKQSVAAEALVSSTASSEALVVARRAALAIAERELANTQIHAPNDGRVVGLTVSAGEFVAPDQAIF
TLINTEHWHASAFFRETELKHIKVGDCATVYVMADRQRAIQGRVEGIGWGVSSEDMLNIPRGLPYVPKSLNWVRVVQRFP
VRISLEKPPEDLMRIGATAVVIVRNDDGC

Sequences:

>Translated_349_residues
MNKQQGSGRKRQLALIVAGVIIIAAAISGWLSVRQTTLNPLSEDAELGASVVHIASSVPGRIISINVEENSKVRRGDLLF
SIEPDLYRLQVEQAQAELKMAEATHDTQQRTVVAERSNAAITNEQIVRAQANLKLATQTLARLQPLRPKGYVTAQQVDDA
ATAKHDAEVSLKQALKQSVAAEALVSSTASSEALVVARRAALAIAERELANTQIHAPNDGRVVGLTVSAGEFVAPDQAIF
TLINTEHWHASAFFRETELKHIKVGDCATVYVMADRQRAIQGRVEGIGWGVSSEDMLNIPRGLPYVPKSLNWVRVVQRFP
VRISLEKPPEDLMRIGATAVVIVRNDDGC
>Mature_349_residues
MNKQQGSGRKRQLALIVAGVIIIAAAISGWLSVRQTTLNPLSEDAELGASVVHIASSVPGRIISINVEENSKVRRGDLLF
SIEPDLYRLQVEQAQAELKMAEATHDTQQRTVVAERSNAAITNEQIVRAQANLKLATQTLARLQPLRPKGYVTAQQVDDA
ATAKHDAEVSLKQALKQSVAAEALVSSTASSEALVVARRAALAIAERELANTQIHAPNDGRVVGLTVSAGEFVAPDQAIF
TLINTEHWHASAFFRETELKHIKVGDCATVYVMADRQRAIQGRVEGIGWGVSSEDMLNIPRGLPYVPKSLNWVRVVQRFP
VRISLEKPPEDLMRIGATAVVIVRNDDGC

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790519, Length=308, Percent_Identity=40.5844155844156, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI87081951, Length=323, Percent_Identity=25.6965944272446, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1789637, Length=324, Percent_Identity=26.5432098765432, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788711, Length=356, Percent_Identity=25.2808988764045, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789041, Length=353, Percent_Identity=27.4787535410765, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789900, Length=276, Percent_Identity=25.7246376811594, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37923; Mature: 37923

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQQGSGRKRQLALIVAGVIIIAAAISGWLSVRQTTLNPLSEDAELGASVVHIASSVPG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHHCCCC
RIISINVEENSKVRRGDLLFSIEPDLYRLQVEQAQAELKMAEATHDTQQRTVVAERSNAA
EEEEEEECCCCCEEECCEEEEECCCCEEEEHHHHHHHHHHHHHCCCHHHHEEEEECCCCC
ITNEQIVRAQANLKLATQTLARLQPLRPKGYVTAQQVDDAATAKHDAEVSLKQALKQSVA
CCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEHHHCCCHHHCCCCCHHHHHHHHHHHHH
AEALVSSTASSEALVVARRAALAIAERELANTQIHAPNDGRVVGLTVSAGEFVAPDQAIF
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCCCCCHHEE
TLINTEHWHASAFFRETELKHIKVGDCATVYVMADRQRAIQGRVEGIGWGVSSEDMLNIP
EEECCCCEEHHHHHHHCCCCEEEECCCEEEEEEECCHHHHHCCCCEEECCCCCCHHHCCC
RGLPYVPKSLNWVRVVQRFPVRISLEKPPEDLMRIGATAVVIVRNDDGC
CCCCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHCCCEEEEEEECCCCC
>Mature Secondary Structure
MNKQQGSGRKRQLALIVAGVIIIAAAISGWLSVRQTTLNPLSEDAELGASVVHIASSVPG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHHCCCC
RIISINVEENSKVRRGDLLFSIEPDLYRLQVEQAQAELKMAEATHDTQQRTVVAERSNAA
EEEEEEECCCCCEEECCEEEEECCCCEEEEHHHHHHHHHHHHHCCCHHHHEEEEECCCCC
ITNEQIVRAQANLKLATQTLARLQPLRPKGYVTAQQVDDAATAKHDAEVSLKQALKQSVA
CCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEHHHCCCHHHCCCCCHHHHHHHHHHHHH
AEALVSSTASSEALVVARRAALAIAERELANTQIHAPNDGRVVGLTVSAGEFVAPDQAIF
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCCCCCHHEE
TLINTEHWHASAFFRETELKHIKVGDCATVYVMADRQRAIQGRVEGIGWGVSSEDMLNIP
EEECCCCEEHHHHHHHCCCCEEEECCCEEEEEEECCHHHHHCCCCEEECCCCCCHHHCCC
RGLPYVPKSLNWVRVVQRFPVRISLEKPPEDLMRIGATAVVIVRNDDGC
CCCCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]