Click here to switch to the map view.

The map label for this gene is malF

Identifier: 22123948

GI number: 22123948

Start: 41435

End: 43027

Strand: Reverse

Name: malF

Synonym: y0027

Alternate gene names: 22123948

Gene position: 43027-41435 (Counterclockwise)

Preceding gene: 22123949

Following gene: 22123947

Centisome position: 0.94

GC content: 48.27

Gene sequence:

>1593_bases
ATGAAAGGGAACATTATGCAGTTATCCCACACCGAGCTTCAAAGCCGAAAAAAGAAAATCGCCTGGTGGCAGAGTGATGC
GCTTAAATGGTTGGTTATCAGCCTGTTAAGCCTCTTCACCTGTTATTTGATCGTGTTGATGTATGCTCAAGGTGAATATC
TTTTTGCCATCGTGACGCTGATTTTGGTGAGTTTAGGGCTGTATGTGTTTGCCAACCGCCGCGCTTATGCCTGGCGCTAT
GTTTACCCCGGCGTGGCCGGTATGGGGCTGTTTGTTCTCTTCCCCTTAATTTGTACTATCGCCATCGCCTTTACTAACTA
CAGCAGTACCAACCAATTAACCTTCGAACGTGCTCAATCCGTCTTGCTGGATCGGCAGTTCCAGACCGGTAAAACATTTA
CCTTTGGTCTCTATCCCAGCGATAACCAATGGCGTTTACAGCTTACCAACCCAGACGACGGTAGCCTGTTCATCTCTGAA
CCCTTCAGTTTTGAGGCCACGGGTGAGCAAAAAGTGATGGTCGCCCCAACCAATACGGCACAAACGAGTGAACCGGCATC
ACTGCGGATTATTACCCAGAGTCGTCAGGCGCTAAGTAGCTTAGTGGCTATCCTGCCTGATGGTGCTGAACTGCGTATGA
GTTCATTACGTCAATTTTCCGGCACCAAGCCGCTCTATACTCTGGGTGCCGATGGTAAAGAACTGATTAATCAGCAAACC
GGCGTGAAATATTGGCCGAATCCTAGTACCGGGTTCTATCAAGCGGTCAACGCTGATGGGCAGTGGGAAAATGAAAAGCT
TAGCCCCGGCTTTACCGTGTCTATCGGCTGGAAAAACTTCCTGCGGGTACTGCACGACGAAGGGATTCAAAAGCCGTTCA
TCTCGATCTTTATCTGGACTATCCTCTTTTCGGTAATGAGCGTGACCTTTACGGTAGCTGTCGGCATGGTGCTGGCATGT
GTGGTGCAATGGGATTCGCTGAAAGGTAAAGCTATCTATCGAGTGATGCTGATCCTGCCCTATGCGGTACCGTCGTTTAT
CTCTATTTTGATTTTCAAAGGGTTATTTAACCAAAGTTTCGGTGAAATCAACCTGATGCTCAGCCATTTATTTGGCATCA
AACCCGCCTGGTTCAGTGATCCTATCACCGCCAAAAGCATGATTTTGATCGTGAATACCTGGCTCGGTTATCCCTACATG
ATGATTTTGTGCATGGGGCTGTTGAAAGCGATCCCTGACGACTTGTATGAGGCATCGGCAATGGATGGCGCAGGGCCATT
CCAGAACTTTTTCCGCATCACATTTCCGTTACTGATTAAGCCACTGACACCGCTGATGATTGCCAGTTTTGCCTTTAACT
TTAATAACTTCGTGCTAATCCAGCTATTGACCAACGGGGGGCCCGATATGATTGGTACCAGCACCCCAGCGGGGTATACC
GATTTGCTGGTTAACTATACCTATCGTGTCGCCTTTGAAGGCGAAGGCGGGCAGGACTTCGGTTTGGCAGCCGCTATCGC
AACCCTGATTTTCATTTTGGTAGGCGCACTCGCGATACTGAATTTGAAAGCCAGCAAAATGAATTTTGACTAA

Upstream 100 bases:

>100_bases
AGCACAGCTCAACGTTAAACACGACCCAACGTTAAACACGGCTCAACTTTAAGCACAACTTATTAAGTTCAAGCACAACT
TAACTTCAAGCACAAAAAGT

Downstream 100 bases:

>100_bases
GGAGGAGATGCAGATGGCTATGGTTCAACCTAAATCCCAGCGTTTGCGTCTATTGGGCACGCATTTTCTGATGTTGTGCT
TTATCGCGCTGATCATGTTC

Product: maltose transporter membrane protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MKGNIMQLSHTELQSRKKKIAWWQSDALKWLVISLLSLFTCYLIVLMYAQGEYLFAIVTLILVSLGLYVFANRRAYAWRY
VYPGVAGMGLFVLFPLICTIAIAFTNYSSTNQLTFERAQSVLLDRQFQTGKTFTFGLYPSDNQWRLQLTNPDDGSLFISE
PFSFEATGEQKVMVAPTNTAQTSEPASLRIITQSRQALSSLVAILPDGAELRMSSLRQFSGTKPLYTLGADGKELINQQT
GVKYWPNPSTGFYQAVNADGQWENEKLSPGFTVSIGWKNFLRVLHDEGIQKPFISIFIWTILFSVMSVTFTVAVGMVLAC
VVQWDSLKGKAIYRVMLILPYAVPSFISILIFKGLFNQSFGEINLMLSHLFGIKPAWFSDPITAKSMILIVNTWLGYPYM
MILCMGLLKAIPDDLYEASAMDGAGPFQNFFRITFPLLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDMIGTSTPAGYT
DLLVNYTYRVAFEGEGGQDFGLAAAIATLIFILVGALAILNLKASKMNFD

Sequences:

>Translated_530_residues
MKGNIMQLSHTELQSRKKKIAWWQSDALKWLVISLLSLFTCYLIVLMYAQGEYLFAIVTLILVSLGLYVFANRRAYAWRY
VYPGVAGMGLFVLFPLICTIAIAFTNYSSTNQLTFERAQSVLLDRQFQTGKTFTFGLYPSDNQWRLQLTNPDDGSLFISE
PFSFEATGEQKVMVAPTNTAQTSEPASLRIITQSRQALSSLVAILPDGAELRMSSLRQFSGTKPLYTLGADGKELINQQT
GVKYWPNPSTGFYQAVNADGQWENEKLSPGFTVSIGWKNFLRVLHDEGIQKPFISIFIWTILFSVMSVTFTVAVGMVLAC
VVQWDSLKGKAIYRVMLILPYAVPSFISILIFKGLFNQSFGEINLMLSHLFGIKPAWFSDPITAKSMILIVNTWLGYPYM
MILCMGLLKAIPDDLYEASAMDGAGPFQNFFRITFPLLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDMIGTSTPAGYT
DLLVNYTYRVAFEGEGGQDFGLAAAIATLIFILVGALAILNLKASKMNFD
>Mature_530_residues
MKGNIMQLSHTELQSRKKKIAWWQSDALKWLVISLLSLFTCYLIVLMYAQGEYLFAIVTLILVSLGLYVFANRRAYAWRY
VYPGVAGMGLFVLFPLICTIAIAFTNYSSTNQLTFERAQSVLLDRQFQTGKTFTFGLYPSDNQWRLQLTNPDDGSLFISE
PFSFEATGEQKVMVAPTNTAQTSEPASLRIITQSRQALSSLVAILPDGAELRMSSLRQFSGTKPLYTLGADGKELINQQT
GVKYWPNPSTGFYQAVNADGQWENEKLSPGFTVSIGWKNFLRVLHDEGIQKPFISIFIWTILFSVMSVTFTVAVGMVLAC
VVQWDSLKGKAIYRVMLILPYAVPSFISILIFKGLFNQSFGEINLMLSHLFGIKPAWFSDPITAKSMILIVNTWLGYPYM
MILCMGLLKAIPDDLYEASAMDGAGPFQNFFRITFPLLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDMIGTSTPAGYT
DLLVNYTYRVAFEGEGGQDFGLAAAIATLIFILVGALAILNLKASKMNFD

Specific function: Part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1790465, Length=513, Percent_Identity=75.4385964912281, Blast_Score=791, Evalue=0.0,
Organism=Escherichia coli, GI1787570, Length=258, Percent_Identity=27.5193798449612, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789861, Length=254, Percent_Identity=28.740157480315, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MALF_YERPE (Q74RF9)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AC0452
- RefSeq:   NP_667371.1
- RefSeq:   NP_994371.1
- RefSeq:   YP_002348596.1
- ProteinModelPortal:   Q74RF9
- SMR:   Q74RF9
- GeneID:   1144974
- GeneID:   1176534
- GeneID:   2765161
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO3715
- KEGG:   ypk:y0027
- KEGG:   ypm:YP_3077
- HOGENOM:   HBG429431
- OMA:   TYRIAFQ
- ProtClustDB:   PRK10999
- BioCyc:   YPES187410:Y0027-MONOMER
- BioCyc:   YPES214092:YPO3715-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 59093; Mature: 59093

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xbb75aa4)-; HASH(0x946ce94)-; HASH(0xb3735ec)-; HASH(0xb37b2c4)-; HASH(0xb196a58)-; HASH(0xb364268)-; HASH(0xb378608)-; HASH(0xb199008)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGNIMQLSHTELQSRKKKIAWWQSDALKWLVISLLSLFTCYLIVLMYAQGEYLFAIVTL
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILVSLGLYVFANRRAYAWRYVYPGVAGMGLFVLFPLICTIAIAFTNYSSTNQLTFERAQS
HHHHHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VLLDRQFQTGKTFTFGLYPSDNQWRLQLTNPDDGSLFISEPFSFEATGEQKVMVAPTNTA
HHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCEEEEECCCCC
QTSEPASLRIITQSRQALSSLVAILPDGAELRMSSLRQFSGTKPLYTLGADGKELINQQT
CCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCC
GVKYWPNPSTGFYQAVNADGQWENEKLSPGFTVSIGWKNFLRVLHDEGIQKPFISIFIWT
CCEECCCCCCCCEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCCCHHHHHHHHH
ILFSVMSVTFTVAVGMVLACVVQWDSLKGKAIYRVMLILPYAVPSFISILIFKGLFNQSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GEINLMLSHLFGIKPAWFSDPITAKSMILIVNTWLGYPYMMILCMGLLKAIPDDLYEASA
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHCHHHHHHHHHHHHHHCCHHHHHHHC
MDGAGPFQNFFRITFPLLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDMIGTSTPAGYT
CCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHH
DLLVNYTYRVAFEGEGGQDFGLAAAIATLIFILVGALAILNLKASKMNFD
HHHEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCH
>Mature Secondary Structure
MKGNIMQLSHTELQSRKKKIAWWQSDALKWLVISLLSLFTCYLIVLMYAQGEYLFAIVTL
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILVSLGLYVFANRRAYAWRYVYPGVAGMGLFVLFPLICTIAIAFTNYSSTNQLTFERAQS
HHHHHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VLLDRQFQTGKTFTFGLYPSDNQWRLQLTNPDDGSLFISEPFSFEATGEQKVMVAPTNTA
HHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCEEEEECCCCC
QTSEPASLRIITQSRQALSSLVAILPDGAELRMSSLRQFSGTKPLYTLGADGKELINQQT
CCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCC
GVKYWPNPSTGFYQAVNADGQWENEKLSPGFTVSIGWKNFLRVLHDEGIQKPFISIFIWT
CCEECCCCCCCCEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCCCHHHHHHHHH
ILFSVMSVTFTVAVGMVLACVVQWDSLKGKAIYRVMLILPYAVPSFISILIFKGLFNQSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GEINLMLSHLFGIKPAWFSDPITAKSMILIVNTWLGYPYMMILCMGLLKAIPDDLYEASA
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHCHHHHHHHHHHHHHHCCHHHHHHHC
MDGAGPFQNFFRITFPLLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDMIGTSTPAGYT
CCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHH
DLLVNYTYRVAFEGEGGQDFGLAAAIATLIFILVGALAILNLKASKMNFD
HHHEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430