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The map label for this gene is queA [H]

Identifier: 21910602

GI number: 21910602

Start: 1109788

End: 1110816

Strand: Direct

Name: queA [H]

Synonym: SpyM3_1066

Alternate gene names: 21910602

Gene position: 1109788-1110816 (Clockwise)

Preceding gene: 21910590

Following gene: 21910603

Centisome position: 58.39

GC content: 42.27

Gene sequence:

>1029_bases
ATGAATACTAACGATTTTGATTTTGAGTTACCAGAAGAATTGATTGCCCAAACCCCACTCGAAAAAAGAGACAGTTCAAA
ACTATTGATTATTGATCACCGTCAAAAAACTATGGTTGATAGCCATTTTGACCATATCATTGATCAATTAAATCCAGGGG
ATGCCCTCGTCATGAATAACACCCGTGTCTTGCCTGCCAGACTTTATGGAGAAAAACCCGATACTCATGGTCATGTGGAA
TTGCTTTTGCTTAAAAATACACAAGGAGATCAATGGGAAGTTTTAGCCAAGCCAGCCAAACGGCTCAAGGTTGGTAGCCA
AGTCAATTTTGGAGATGGGCATCTCAAAGCCACCATTATCGACGAATTAGAACATGGTGGACGAATTGTTGAATTCAGCT
ATGATGGGATTTTCTTAGAAGTTTTAGAGAGCCTAGGCGAAATGCCATTGCCTCCTTATATTCACGAAAAATTAGAGGAT
GCTGAGCGATACCAAACCGTTTATGCTAAAGAAAATGGCTCTGCGGCCGCCCCAACTGCTGGTCTCCACTTCACAACAGA
CTTGCTCAAAAAAATCGAAGCCAAAGGGGTACATTTGGTTTATTTGACCCTACATGTAGGGCTTGGGACTTTTAGACCTG
TTTCCGTGGATAACCTCGATGAGCATGATATGCATTCGGAATTTTATTCCCTCTCAGAAGAAGCAGCGCAAACCCTAAGA
GACGTAAAACAAGCTGGCGGTCGTGTTGTCGCTGTAGGAACCACCTCTATTCGTACGTTGGAAACCATCGGAAGTAAGTT
CCAAGGGGACATTCAAGCTGATTCAGGTTGGACCAATATTTTTATTAAACCAGGCTACCAATTCAAGGTAGTTGATGCTT
TTTCAACTAATTTCCATCTTCCAAAATCAACCCTTGTGATGCTAGTATCAGCCTTTGCAGGGCGTGACTTTGTCCTTGAA
GCTTACAGACACGCGGTTGACGAAAAATATCGTTTCTTTAGCTTTGGCGATGCCATGTTTGTCAACTAA

Upstream 100 bases:

>100_bases
AAGATGACGGGTTAGACGACAAGTGATATAATAGAAAAGATTTATCTTACATGAAAGAGTTACTAGGAAGAGATTTACTA
GTGGCTTTAAAGGAGCCCCA

Downstream 100 bases:

>100_bases
AAACCTATGAAATTACTTGGAAAATGGCTATTCTTCTTGATGTTTCTGACTTTTTGCGTCAGTTTTTGGCAGAAAGACTA
TCAGCGGATTGCCTTTGAAA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFDHIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVE
LLLLKNTQGDQWEVLAKPAKRLKVGSQVNFGDGHLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDNLDEHDMHSEFYSLSEEAAQTLR
DVKQAGGRVVAVGTTSIRTLETIGSKFQGDIQADSGWTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLE
AYRHAVDEKYRFFSFGDAMFVN

Sequences:

>Translated_342_residues
MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFDHIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVE
LLLLKNTQGDQWEVLAKPAKRLKVGSQVNFGDGHLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDNLDEHDMHSEFYSLSEEAAQTLR
DVKQAGGRVVAVGTTSIRTLETIGSKFQGDIQADSGWTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLE
AYRHAVDEKYRFFSFGDAMFVN
>Mature_342_residues
MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFDHIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVE
LLLLKNTQGDQWEVLAKPAKRLKVGSQVNFGDGHLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDNLDEHDMHSEFYSLSEEAAQTLR
DVKQAGGRVVAVGTTSIRTLETIGSKFQGDIQADSGWTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLE
AYRHAVDEKYRFFSFGDAMFVN

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=47.8386167146974, Blast_Score=312, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38364; Mature: 38364

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFDHIIDQLNPGDALVMNN
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEECC
TRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRLKVGSQVNFGDGHLKATII
CEEEEHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHHCCCCEECCCCCCEEEEEE
DELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLEDAERYQTVYAKENGSAAAPTA
HHHHCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCC
GLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDNLDEHDMHSEFYSLSEEAAQTLR
CCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DVKQAGGRVVAVGTTSIRTLETIGSKFQGDIQADSGWTNIFIKPGYQFKVVDAFSTNFHL
HHHHCCCEEEEEECCCHHHHHHHHHHHCCCEECCCCCEEEEECCCCEEEEEEECCCCCCC
PKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFSFGDAMFVN
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCEEECC
>Mature Secondary Structure
MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFDHIIDQLNPGDALVMNN
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEECC
TRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRLKVGSQVNFGDGHLKATII
CEEEEHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHHCCCCEECCCCCCEEEEEE
DELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLEDAERYQTVYAKENGSAAAPTA
HHHHCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCC
GLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDNLDEHDMHSEFYSLSEEAAQTLR
CCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DVKQAGGRVVAVGTTSIRTLETIGSKFQGDIQADSGWTNIFIKPGYQFKVVDAFSTNFHL
HHHHCCCEEEEEECCCHHHHHHHHHHHCCCEECCCCCEEEEECCCCEEEEEEECCCCCCC
PKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFSFGDAMFVN
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]