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The map label for this gene is pfkA [H]

Identifier: 21910449

GI number: 21910449

Start: 969849

End: 970862

Strand: Reverse

Name: pfkA [H]

Synonym: SpyM3_0913

Alternate gene names: 21910449

Gene position: 970862-969849 (Counterclockwise)

Preceding gene: 21910450

Following gene: 21910448

Centisome position: 51.08

GC content: 43.2

Gene sequence:

>1014_bases
ATGAAACGTATTGCTGTTTTAACCAGTGGCGGCGACGCTCCTGGTATGAATGCTGCTATTCGTGCAGTCGTTCGTAAAGC
AATTTCAGAAGGAATGGAAGTTTATGGTATCAACCGTGGCTATGCAGGTATGGTTGATGGCGACATTTTCCCACTGGGTT
CAAAAGAAGTTGGTGATAAAATCTCACGTGGTGGAACTTTCTTATACTCAGCTCGTTATCCAGAATTTGCTCAACTTGAA
GGTCAATTGGCTGGTATTGAGCAACTTAAAAAACATGGTATTGAGGGTGTTGTTGTTATTGGTGGGGATGGTTCTTATCA
TGGCGCTATGCGCTTGACAGAGCACGGCTTCCCAGCGGTAGGTATCCCAGGAACAATCGATAACGATATTGCGGGAACTG
ACTACACTATCGGATTTGATACAGCAGTTAACACAGCAGTTGAAGCTATTGATAAACTTCGTGACACCTCATCAAGTCAC
GGACGTACTTTTGTGGTTGAAGTGATGGGTCGCAATGCGGGTGATATTGCTCTTTGGGCAGGTATTGCTAGTGGTGCTGA
TCAAATCATTGTTCCAGAAGAAGAGTTTGATATTGAAAAAGTGGCATCAACAATCCAATATGATTTTGAACACAAAGGTA
AAAATCACCACATTATCGTCTTAGCTGAAGGTGTTATGAGCGGTGAAGCTTTTGCTCAAAAACTGAAAGAAGCTGGCGAT
AAGAGTGACCTTCGTGTGACAAATCTTGGTCACATTCTTCGTGGGGGTTCACCAACTGCTCGTGACCGTGTCATTGCTTC
TTGGATGGGTTCACATGCTGTTGAGCTACTTAAAGAAGGCAAAGGTGGTTTGGCTGTTGGTATTCACAATGAAGAACTTG
TTGAAAGCCCAATCCTTGGTACTGCAGAAGAAGGTGCTTTGTTCAGCTTGACTGAAGAAGGCCAGATTATTGTTAATAAT
CCACACAAAGCTCGTTTAGACTTTGCAGCTTTGAACCGTTCATTGTCACAATAA

Upstream 100 bases:

>100_bases
CTGAAAACGGTTTTTCGATAAAAATGGACAATTAGTGAAGTATTTTGCAAATGAATGTGCTATAATAAGACCGTTAAATT
AATTAAAGGAGTACCAGAAA

Downstream 100 bases:

>100_bases
GTTTAGTTAAAATTGTTAAATCGTCAATTATTGACAAAAAAATAAGGGAGTTTCAGAGTAAAATGAATAAACGCGTAAAA
ATCGTTGCAACACTTGGTCC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLE
GQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHIIVLAEGVMSGEAFAQKLKEAGD
KSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVELLKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGQIIVNN
PHKARLDFAALNRSLSQ

Sequences:

>Translated_337_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLE
GQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHIIVLAEGVMSGEAFAQKLKEAGD
KSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVELLKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGQIIVNN
PHKARLDFAALNRSLSQ
>Mature_337_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLE
GQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHIIVLAEGVMSGEAFAQKLKEAGD
KSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVELLKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGQIIVNN
PHKARLDFAALNRSLSQ

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=339, Percent_Identity=39.5280235988201, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI48762920, Length=317, Percent_Identity=40.0630914826498, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI266453768, Length=336, Percent_Identity=38.6904761904762, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI266453748, Length=336, Percent_Identity=38.6904761904762, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI4505749, Length=336, Percent_Identity=38.6904761904762, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI266453619, Length=340, Percent_Identity=37.6470588235294, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1790350, Length=304, Percent_Identity=55.921052631579, Blast_Score=315, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI25147584, Length=343, Percent_Identity=37.3177842565598, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17558788, Length=310, Percent_Identity=35.8064516129032, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI72003601, Length=288, Percent_Identity=31.9444444444444, Blast_Score=147, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6321679, Length=336, Percent_Identity=36.9047619047619, Blast_Score=186, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323861, Length=337, Percent_Identity=35.6083086053413, Blast_Score=179, Evalue=7e-46,
Organism=Drosophila melanogaster, GI28573326, Length=338, Percent_Identity=39.0532544378698, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24652337, Length=339, Percent_Identity=38.6430678466077, Blast_Score=200, Evalue=9e-52,
Organism=Drosophila melanogaster, GI17647809, Length=340, Percent_Identity=38.5294117647059, Blast_Score=200, Evalue=1e-51,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35763; Mature: 35763

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDK
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEECCCHHHHHH
ISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAV
HHCCCEEEEECCCCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSHGRTFVVEVMGRNAGDIALWA
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEE
GIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHIIVLAEGVMSGEAFAQKLKEAGD
ECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCC
KSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVELLKEGKGGLAVGIHNEELVESPILG
CCCEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCC
TAEEGALFSLTEEGQIIVNNPHKARLDFAALNRSLSQ
CCCCCCEEEEECCCEEEEECCCHHEEHHHHHHHHHCC
>Mature Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDK
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEECCCHHHHHH
ISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAV
HHCCCEEEEECCCCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSHGRTFVVEVMGRNAGDIALWA
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEE
GIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHIIVLAEGVMSGEAFAQKLKEAGD
ECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCC
KSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVELLKEGKGGLAVGIHNEELVESPILG
CCCEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCC
TAEEGALFSLTEEGQIIVNNPHKARLDFAALNRSLSQ
CCCCCCEEEEECCCEEEEECCCHHEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA