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The map label for this gene is pyk [H]

Identifier: 21910448

GI number: 21910448

Start: 968284

End: 969786

Strand: Reverse

Name: pyk [H]

Synonym: SpyM3_0912

Alternate gene names: 21910448

Gene position: 969786-968284 (Counterclockwise)

Preceding gene: 21910449

Following gene: 21910447

Centisome position: 51.03

GC content: 40.25

Gene sequence:

>1503_bases
ATGAATAAACGCGTAAAAATCGTTGCAACACTTGGTCCTGCGGTTGAAATCCGTGGTGGTAAAAAATACGGAGAAGATGG
CTACTGGGCTGGTCAACTTGATGTTGAAGAATCAGCAAAAAAAATCGCTGAATTAATTGAAGCTGGTGCGAATGTTTTCC
GTTTCAATTTCTCACATGGTGACCACAAAGAACAAGGTGACCGTATGGCAACTGTTCGCCTTGCAGAAGAAATTGCTCGT
CAAAAAGTTGGTTTCCTTTTGGATACTAAAGGTCCTGAAATGCGTACGGAATTGTTTGCAGATGATGCTAAAGAATTCAG
CTATGTCACTGGAGAAAAAATCCGTGTAGCGACTACACAAGGTATTCAATCAACTCGCGATGTTATTGCTTTGAACGTTG
CTGGTAGCCTTGACATCTACGATGAAGTAGAAGTAGGTCATACTATTCTTATCGATGATGGTAAACTCGGTTTGAAAGTG
ATCGACAAAGATATCGCAACACGTCAATTTATCGTTGAAGTAGAAAACGATGGTATTATTGCTAAACAAAAAGGTGTTAA
TATCCCTAACACTAAAATTCCTTTCCCAGCACTTGCGGAACGCGACAATGCTGATATCCGTTTTGGTCTTGAACAAGGAC
TTAACTTTATTGCAATCTCATTTGTTCGTACAGCCAAAGACGTTGAGGAAGTTCGTGAAATCTGTCGCGAAACTGGTAAC
GATCATGTGCAATTATTTGCTAAGATTGAAAACCAACAAGGTATCGATAATCTTGATGAAATCATCGAAGCTGCTGATGG
TATCATGATTGCACGTGGCGACATGGGTATCGAAGTACCATTTGAAATGGTTCCAGTTTTCCAAAAAATGATTATCACTA
AAGTGAATGCAGCTGGTAAAGCAGTTATCACAGCAACAAACATGCTTGAAACAATGACTGAAAAACCACGTGCGACACGT
TCTGAAGTATCTGACGTCTTCAATGCTGTTATCGATGGTACTGATGCAACAATGCTTTCAGGAGAGTCAGCTAACGGTAA
ATACCCGGTTGAATCTGTTCGTACAATGGCGACTATTGATCGTAATGCACAAACTCTTCTTAACGAATATGGTCGTTTAG
ATTCATCTGCCTTCCCACGTACCAATAAAACAGATGTTATTGCTTCAGCTGTTAAAGATGCCACTCATTCAATGGACATC
AAACTTGTTGTGACTATTACTGAGACAGGTAATACAGCTCGTGCGATTTCTAAATTCCGTCCAGATGCGGATATCTTAGC
TGTAACATTTGATGAAAAAGTACAACGTGCTTTGATGATTAACTGGGCTGTTATTCCTGTTCTTGCTGAAAAACCTGCAT
CTACTGATGACATGTTTGAAGTAGCAGAGCGTGTTGCTGTGGAGGCAGGCTTAGTTCAATCTGGGGATAATATTGTTATT
GTTGCTGGTGTTCCAGTTGGTACTGGTGGAACGAATACTATGCGTGTTCGTACAGTAAAATAA

Upstream 100 bases:

>100_bases
TAGACTTTGCAGCTTTGAACCGTTCATTGTCACAATAAGTTTAGTTAAAATTGTTAAATCGTCAATTATTGACAAAAAAA
TAAGGGAGTTTCAGAGTAAA

Downstream 100 bases:

>100_bases
ATGTGTTATAACTATTTACCTAAAGGTTAGAAGGCAAGCTTCTAACCTTTTTTGATATCAAAAATATCATGTTAATTCAC
TAGTGTTAATTTACTTTAAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAELIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIAR
QKVGFLLDTKGPEMRTELFADDAKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
DHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDI
KLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWAVIPVLAEKPASTDDMFEVAERVAVEAGLVQSGDNIVI
VAGVPVGTGGTNTMRVRTVK

Sequences:

>Translated_500_residues
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAELIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIAR
QKVGFLLDTKGPEMRTELFADDAKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
DHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDI
KLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWAVIPVLAEKPASTDDMFEVAERVAVEAGLVQSGDNIVI
VAGVPVGTGGTNTMRVRTVK
>Mature_500_residues
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAELIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIAR
QKVGFLLDTKGPEMRTELFADDAKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
DHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDI
KLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWAVIPVLAEKPASTDDMFEVAERVAVEAGLVQSGDNIVI
VAGVPVGTGGTNTMRVRTVK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=480, Percent_Identity=38.125, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI33286420, Length=480, Percent_Identity=38.125, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI33286418, Length=483, Percent_Identity=38.0952380952381, Blast_Score=310, Evalue=3e-84,
Organism=Homo sapiens, GI10835121, Length=513, Percent_Identity=36.0623781676413, Blast_Score=308, Evalue=9e-84,
Organism=Homo sapiens, GI32967597, Length=513, Percent_Identity=36.0623781676413, Blast_Score=308, Evalue=1e-83,
Organism=Homo sapiens, GI310128732, Length=279, Percent_Identity=39.7849462365591, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI310128730, Length=279, Percent_Identity=39.7849462365591, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI310128736, Length=258, Percent_Identity=32.9457364341085, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI310128734, Length=258, Percent_Identity=32.9457364341085, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI310128738, Length=220, Percent_Identity=35.4545454545455, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1787965, Length=501, Percent_Identity=39.9201596806387, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1788160, Length=512, Percent_Identity=33.0078125, Blast_Score=220, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17544584, Length=510, Percent_Identity=37.843137254902, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI71984413, Length=513, Percent_Identity=38.0116959064327, Blast_Score=298, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17506829, Length=513, Percent_Identity=38.0116959064327, Blast_Score=298, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI17506831, Length=511, Percent_Identity=38.160469667319, Blast_Score=298, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI71984406, Length=513, Percent_Identity=38.0116959064327, Blast_Score=297, Evalue=7e-81,
Organism=Saccharomyces cerevisiae, GI6319279, Length=507, Percent_Identity=35.7001972386588, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6324923, Length=509, Percent_Identity=36.5422396856582, Blast_Score=289, Evalue=6e-79,
Organism=Drosophila melanogaster, GI24648964, Length=519, Percent_Identity=36.6088631984586, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI28571814, Length=519, Percent_Identity=36.6088631984586, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=37.6811594202899, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24581235, Length=474, Percent_Identity=31.8565400843882, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24646914, Length=328, Percent_Identity=32.9268292682927, Blast_Score=173, Evalue=3e-43,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 54550; Mature: 54550

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAELIEAGANVFRFNFSHG
CCCEEEEEEEECCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCC
DHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADDAKEFSYVTGEKIRVATTQ
CCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCHHHHHEECCCEEEEEECC
GIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKVIDKDIATRQFIVEVENDGII
CCCCCCCEEEEEECCCCCCCEECCCCCEEEEECCCCCEEEECCHHCCEEEEEEECCCCEE
AKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
EECCCCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCC
DHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGK
CEEEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
AVITATNMLETMTEKPRATRSEVSDVFNAVIDGTDATMLSGESANGKYPVESVRTMATID
EEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHC
RNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDIKLVVTITETGNTARAISKFR
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHC
PDADILAVTFDEKVQRALMINWAVIPVLAEKPASTDDMFEVAERVAVEAGLVQSGDNIVI
CCCCEEEEEEHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCEEE
VAGVPVGTGGTNTMRVRTVK
EEECCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAELIEAGANVFRFNFSHG
CCCEEEEEEEECCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCC
DHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADDAKEFSYVTGEKIRVATTQ
CCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCHHHHHEECCCEEEEEECC
GIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKVIDKDIATRQFIVEVENDGII
CCCCCCCEEEEEECCCCCCCEECCCCCEEEEECCCCCEEEECCHHCCEEEEEEECCCCEE
AKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
EECCCCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCC
DHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGK
CEEEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
AVITATNMLETMTEKPRATRSEVSDVFNAVIDGTDATMLSGESANGKYPVESVRTMATID
EEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHC
RNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDIKLVVTITETGNTARAISKFR
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHC
PDADILAVTFDEKVQRALMINWAVIPVLAEKPASTDDMFEVAERVAVEAGLVQSGDNIVI
CCCCEEEEEEHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCEEE
VAGVPVGTGGTNTMRVRTVK
EEECCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8478320; 11337471 [H]