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The map label for this gene is yqiG [H]

Identifier: 21910395

GI number: 21910395

Start: 915643

End: 916842

Strand: Reverse

Name: yqiG [H]

Synonym: SpyM3_0859

Alternate gene names: 21910395

Gene position: 916842-915643 (Counterclockwise)

Preceding gene: 21910396

Following gene: 21910394

Centisome position: 48.24

GC content: 40.67

Gene sequence:

>1200_bases
ATGAAAGAAAGATTTAGTCCTTTATTTGAGCCTTTGACTTTGCCAAATGGGAGTCAATTGGATAATCGCTTTGTTTTGTC
TCCTATGGTGACCAACTCATCTACAAAAGACGGTTATGTCACCCAAGATGATGTATCCTATGCTTTGAGACGAGCAGCTT
CTGCCCCTCTACAAATTACAGGAGCGGCCTATGTGGATCCTTATGGTCAATTGTTTGAATATGGTTTTAGTGTTACAAAA
GATGCCGATATTTCTGGCTTGAAAGAGCTAGCTCAAGCGATGAAGGCAAAGGGAGCTAAAGCTGTCTTACAACTGACCCA
CGCTGGCCGGTTTGCTAGCCATGCCTTGACTAAATATGGCTTTGTCTATGGGCCTAGTTACATGCAGTTGAGATCTCCCC
AACCTCATGAAGTCAAACCTTTAACAGGTCAGCAAATAGAGGAATTGATTGCAGCTTACGCTCAGGCCACTCGGCGGGCA
ATTCAAGCAGGCTTTGATGGCGTGGAAGTATCTTCGGCGCAGCGCTTATTGATTCAAACGTTCTTTTCGACTTTTTCGAA
CAAGAGGACAGATAGTTATGGTTGTCAAACACTTTTTAATCGCTCTAAGTTAACTCTAGCTGTTTTGCAAGCTGTTCAAC
AGGTGATTAAACAAGAAGCTCCTGATGGGTTTATTTTTGGATTTCGTGCTACACCAGAAGAAACTAGGGGAAATGATATT
GGTTATAGTATTGACGAGTTTTTGCAGTTGATGGATTGGGTACTTAATGTTGCAAAACTTGATTATTTAGCGATAGCAAG
TTGGGGCCGTCATGTATTTCGAAATACTGTTCGTTCTCCTGGCCCTTATTATGGAAGGCGAGTTAATCAGGTAGTTAGAG
ATTATTTAAGAAATAAGCTACCTGTTATGGCTACAGGGGGAATGAACACACCTGACAAAGCTATTGAAGCATTAGCTCAT
GCTGATTTTATTGGAGTATCTACACCTTTTGTCGTGGATCCAGAATTTGCTCATAAAATCAAAGAAGGGTGTGAAGAGAG
TATTCATTTAAGGATTCGACCTGCTGATTTGAAAAGTTTAGCGATTCCTCAGGCTTCTTTCAAAGATATTGTACCTTTGA
TGGACTACGGGGAATCATTGCCTAAAGAATCAAGAACTTTATTTCGTAGTCTTACTCACAATTATAAGGAGATTAAATGA

Upstream 100 bases:

>100_bases
GCTTTTCCCTCACGGAAAAAAGTCCAGATCAAGCCTTACTGACTAATTTTGATGCTGTCTATGAACGGATGCAGCTAGAA
GTCGTCAGAAAGGAAGGCAA

Downstream 100 bases:

>100_bases
AAGTCAGTATTCTTGATTATGGGGTGATTGATAAAGAAAAAACACCCCAAGAAGCTTTATTAGAAACGCGCTGCTTAGCA
CAAGTAGCCGACAAACTTGG

Product: putative trimethylamine dehydrogenase

Products: dimethylamine; formaldehyde; reduced electron-transferring flavoprotein

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDDVSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTK
DADISGLKELAQAMKAKGAKAVLQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQEAPDGFIFGFRATPEETRGNDI
GYSIDEFLQLMDWVLNVAKLDYLAIASWGRHVFRNTVRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAH
ADFIGVSTPFVVDPEFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLTHNYKEIK

Sequences:

>Translated_399_residues
MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDDVSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTK
DADISGLKELAQAMKAKGAKAVLQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQEAPDGFIFGFRATPEETRGNDI
GYSIDEFLQLMDWVLNVAKLDYLAIASWGRHVFRNTVRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAH
ADFIGVSTPFVVDPEFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLTHNYKEIK
>Mature_399_residues
MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDDVSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTK
DADISGLKELAQAMKAKGAKAVLQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQEAPDGFIFGFRATPEETRGNDI
GYSIDEFLQLMDWVLNVAKLDYLAIASWGRHVFRNTVRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAH
ADFIGVSTPFVVDPEFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLTHNYKEIK

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=355, Percent_Identity=25.6338028169014, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787939, Length=218, Percent_Identity=26.605504587156, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17559804, Length=391, Percent_Identity=25.3196930946292, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17565138, Length=367, Percent_Identity=26.7029972752044, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=363, Percent_Identity=26.7217630853994, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=369, Percent_Identity=24.9322493224932, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=389, Percent_Identity=24.4215938303342, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI72001454, Length=265, Percent_Identity=28.3018867924528, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17566914, Length=265, Percent_Identity=27.9245283018868, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=246, Percent_Identity=28.8617886178862, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=238, Percent_Identity=26.890756302521, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.5.8.2

Molecular weight: Translated: 44510; Mature: 44510

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDDVSYALRRAASAPLQIT
CCCHHHHHHCCCCCCCCCCCCCEEEECEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEC
GAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAVLQLTHAGRFASHALTKYG
CCHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
FVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRAIQAGFDGVEVSSAQRLLIQT
CEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQEAPDGFIFGFRATPEETRGNDI
HHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
GYSIDEFLQLMDWVLNVAKLDYLAIASWGRHVFRNTVRSPGPYYGRRVNQVVRDYLRNKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
PVMATGGMNTPDKAIEALAHADFIGVSTPFVVDPEFAHKIKEGCEESIHLRIRPADLKSL
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCCEEEEEECCCHHHH
AIPQASFKDIVPLMDYGESLPKESRTLFRSLTHNYKEIK
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDDVSYALRRAASAPLQIT
CCCHHHHHHCCCCCCCCCCCCCEEEECEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEC
GAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAVLQLTHAGRFASHALTKYG
CCHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
FVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRAIQAGFDGVEVSSAQRLLIQT
CEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQEAPDGFIFGFRATPEETRGNDI
HHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
GYSIDEFLQLMDWVLNVAKLDYLAIASWGRHVFRNTVRSPGPYYGRRVNQVVRDYLRNKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
PVMATGGMNTPDKAIEALAHADFIGVSTPFVVDPEFAHKIKEGCEESIHLRIRPADLKSL
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCCEEEEEECCCHHHH
AIPQASFKDIVPLMDYGESLPKESRTLFRSLTHNYKEIK
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: trimethylamine; H2O; electron-transferring flavoprotein

Specific reaction: trimethylamine + H2O + electron-transferring flavoprotein = dimethylamine + formaldehyde + reduced electron-transferring flavoprotein

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]