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The map label for this gene is potD [C]

Identifier: 21910283

GI number: 21910283

Start: 800160

End: 801206

Strand: Direct

Name: potD [C]

Synonym: SpyM3_0747

Alternate gene names: 21910283

Gene position: 800160-801206 (Clockwise)

Preceding gene: 21910282

Following gene: 21910284

Centisome position: 42.1

GC content: 34.77

Gene sequence:

>1047_bases
ATGTTAAAACTCAAACGCAAATGGCTATTAAGCTTTTTGTTAGTAATAATTATTCTAGCAATCGTTAACATTGCAATGTA
TATATTCTCATCGTCAAAAAAAGATAGTGCTAAAGAATTAGTTATCTTGACTCCTAATAGCCAAACTATTTTAACAGGGA
CTATTCCAGCCTTTGAGGAAAAGTATGGGGTTAAAGTAAGATTAATTCAAGGTGGGACGGGCCAACTTATTGATCAATTA
GGTCGAAAAGATAAACCATTAAACGCTGATATTTTCTTTGGTGGCAATTACACTCAATTTGAAAGCCATAAAGATTTATT
TGAATCTTATGTTTCTCCGCAGGTTTCTACTGTCATTTCAGATTACCAATTGCCTAGTCATCGCGCAACCCCATATACGA
TCAATGGCAGTGTACTGATTGTTAATAACGAATTAGCAAGAGGACTTCATATTACCAGTTATGAGGATTTGCTACAACCA
GCTTTAAAAGGCAAAATTGCTTTTGCTGACCCAAATAGCTCATCTAGCGCTTTTTCACAATTAACAAACATCTTACTGGC
TAAAGGAGGCTATACGAGCTCAGAAGCTTGGGAGTATGTGAAACGTTTATTACTGTCTATGAATTCTATCAGAGCAACAA
GTTCTTCAGATGTTTACGAATCTGTTGCAGAAGGTAAAATGATTGTTGGTTTGACCTATGAAGATCCTTGCATCAATCTG
CAAAAAAGCGGTGCAAACGTTTCGATTGTTTATCCTGATGAGGGGACAGTATTTGTTCCGTCATCAGTCGCTATTATCAA
AAAAGCAAAGGCAATGAAAGAAGCTAAGCAATTTATTAATTTTATTTTATCATCAGATGTACAAAATGCCTTTGGGCAGT
CAACTAGTAATAGACCTATCCGACGAGATGCTCAAACAAGTCATGAAATGAAACCATTAGATGAAATTATTACTTTAAAA
GAAGATTACGGATATGTTACTAGACATAAGAAAAAAATATTGAATACCTATAATCGACTACATGAAGCATTAGAAAGAGG
AAAGTAG

Upstream 100 bases:

>100_bases
ACTATCGCTATTTTGATAGGGTTTTTAAAAAGTATCTAGGACAAACTGTAAAGGCTTTTAAAGAAGAGCATTTTATGAAA
GATACCGATAGGAGTAAGCC

Downstream 100 bases:

>100_bases
ACTCTAGGCTTGTTGTTGCTATACTAGTAGTGTTACAAAATAGCGTAGTCATTTTTTGAAATCGATTTCATAATCTGGTA
GACTAAGATTATCACGAAAA

Product: putative periplasmic-iron-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MLKLKRKWLLSFLLVIIILAIVNIAMYIFSSSKKDSAKELVILTPNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQL
GRKDKPLNADIFFGGNYTQFESHKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDVYESVAEGKMIVGLTYEDPCINL
QKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAKQFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLK
EDYGYVTRHKKKILNTYNRLHEALERGK

Sequences:

>Translated_348_residues
MLKLKRKWLLSFLLVIIILAIVNIAMYIFSSSKKDSAKELVILTPNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQL
GRKDKPLNADIFFGGNYTQFESHKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDVYESVAEGKMIVGLTYEDPCINL
QKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAKQFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLK
EDYGYVTRHKKKILNTYNRLHEALERGK
>Mature_348_residues
MLKLKRKWLLSFLLVIIILAIVNIAMYIFSSSKKDSAKELVILTPNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQL
GRKDKPLNADIFFGGNYTQFESHKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDVYESVAEGKMIVGLTYEDPCINL
QKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAKQFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLK
EDYGYVTRHKKKILNTYNRLHEALERGK

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787367, Length=180, Percent_Identity=25, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38743; Mature: 38743

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLKRKWLLSFLLVIIILAIVNIAMYIFSSSKKDSAKELVILTPNSQTILTGTIPAFEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCHHH
KYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFESHKDLFESYVSPQVSTVIS
CCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHH
DYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQPALKGKIAFADPNSSSSAFSQ
HCCCCCCCCCCEEECCEEEEEECHHHCCEECCCHHHHHHHHHCCEEEEECCCCCHHHHHH
LTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDVYESVAEGKMIVGLTYEDPCINL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCCE
QKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAKQFINFILSSDVQNAFGQSTSNRPI
ECCCCEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKILNTYNRLHEALERGK
CCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKLKRKWLLSFLLVIIILAIVNIAMYIFSSSKKDSAKELVILTPNSQTILTGTIPAFEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCHHH
KYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFESHKDLFESYVSPQVSTVIS
CCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHH
DYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQPALKGKIAFADPNSSSSAFSQ
HCCCCCCCCCCEEECCEEEEEECHHHCCEECCCHHHHHHHHHCCEEEEECCCCCHHHHHH
LTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDVYESVAEGKMIVGLTYEDPCINL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCCE
QKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAKQFINFILSSDVQNAFGQSTSNRPI
ECCCCEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKILNTYNRLHEALERGK
CCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]