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The map label for this gene is acoL [C]

Identifier: 21910200

GI number: 21910200

Start: 727183

End: 728946

Strand: Direct

Name: acoL [C]

Synonym: SpyM3_0664

Alternate gene names: 21910200

Gene position: 727183-728946 (Clockwise)

Preceding gene: 21910199

Following gene: 21910202

Centisome position: 38.26

GC content: 41.5

Gene sequence:

>1764_bases
ATGGCTGTTGAAATTATTATGCCAAAACTCGGTGTTGACATGCAAGAAGGTGAAATCATCGAGTGGAAAAAACAAGAAGG
TGATACTGTCAATGAAGGCGATATTCTTCTTGAAATCATGTCAGATAAAACCAATATGGAACTTGAAGCAGAAGACTCAG
GTGTTCTTCTTAAAATTACACGTCAAGCAGGTGAAACAGTACCTGTAACAGAAGTTATCGGATATATCGGTGCTGAAGGT
GAATCTGTTGAGGTTTCTAGCCCAGCTGCTTCAGATGTGAATGTTGCTCGTACAACAGAAGATTTAGAAGCTGCTGGACT
TGAAGTGCCAAAAGCACCAGCTCAAGCTGCTTCAGCTGCACCAAAAGCTGCACTTGCTGATGATGAGTATGACATCATCG
TTGTTGGTGGTGGCCCTGCTGGTTATTATGCAGCTATTCGTGGTGCTCAACTTGGTGGTAAAATTGCCATCGTTGAGAAA
TCTGAATTTGGTGGAACTTGCTTGAACGTAGGGTGTATCCCAACCAAAACTTACCTTAAAAATGCTGAAATCCTTGATGG
TATTAAGATTGCAGCAGGACGTGGGATTAACCTTGCCTCAACTAACTATACCATTGACATGGACAAAACAGTTGACTTTA
AAAACACCGTTGTTAAAACCTTAACAGGCGGCGTTCAAGGTCTTCTAAAAGCTAATAAAGTCACTATCTTTAATGGGCTT
GGTCAAGTTAACCCTGACAAGACTGTAACCATTGGGTCACAAACCATTAAGGGTCGCAACGTTATCCTTGCTACAGGTTC
TAAAGTATCACGTATCAATATCCCAGGTATTGACTCTAAACTTGTTTTAACGTCTGACGATATCCTTGACCTCCGTGAAA
TGCCAAAATCACTAGCAGTTATGGGTGGTGGTGTTGTTGGTATCGAGCTTGGACTTGTTTGGGCATCTTACGGTGTGGAT
GTTACCGTTATTGAAATGGCTGACCGCATTATCCCAGCTATGGATAAGGAAGTCTCTCTTGAACTTCAAAAAATCCTTTC
TAAGAAAGGCATGAAGATCAAAACATCTGTTGGTGTCTCTGAAATTGTTGAAGCAAATAACCAATTGACCTTGAAACTTA
ACAATGGTGAAGAAGTTGTCGCTGAAAAAGCTCTTCTTTCTATCGGTCGTGTACCACAAATGAACGGTCTGGAAAATCTT
AACCTTGAAATGGATCGTAACCGTATCAAAGTTAATGACTACCAAGAGACATCAATTCCAGGTATCTATGCACCAGGTGA
CGTTAACGGAACTAAAATGCTTGCTCATGCTGCTTACCGTATGGGTGAAGTGGCTGCAGAAAATGCGATACATGGCAACA
CAACTCGTAAAGCTAACCTTAAATACACACCAGCGGCTGTTTACACACACCCTGAAGTGGCAATGGTTGGTTTAACTGAA
GAACAAGCGCGTGAGCAATATGGCGATGTGCTTATCGGAAAAAATAGCTTTACCGGTAACGGACGCGCGATTGCTTCAAA
TGAAGCACATGGTTTTGTTAAAGTTATTGCTGATGCCAAATACCACGAAATCTTAGGTGTTCATATTATTGGTCCAGCTG
CAGCTGAGATGATTAACGAAGCAGCTACTATTATGGAATCTGAATTAACCGTTGATGAATTGTTATTATCAATTCATGGA
CACCCAACCTTCTCTGAAGTGATGTACGAAGCCTTTGCAGATGTGCTTGGCGAAGCTATCCATAACCCACCAAAACGTAA
GTAA

Upstream 100 bases:

>100_bases
AGTCCTAGTCGTCCTGACGTGGGTGCGTCAACGAAATCAAAGATTTCTGACTTACCGCAAAAATTCAATCGCTTTCTCCT
TGCTCTTGGTATCTTAAATT

Downstream 100 bases:

>100_bases
AACGTCTTTGTCTTATTAACTAATGAAAAATATCATCACATAACAGATCACAAAAAAGACTAGCACAACTATGAACTGCA
CCCCAAACGTTAGATATAAA

Product: putative dihydrolipoamide dehydrogenase component E3

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKTNMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEG
ESVEVSSPAASDVNVARTTEDLEAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVVKTLTGGVQGLLKANKVTIFNGL
GQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPGIDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVD
VTVIEMADRIIPAMDKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVPQMNGLENL
NLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAIHGNTTRKANLKYTPAAVYTHPEVAMVGLTE
EQAREQYGDVLIGKNSFTGNGRAIASNEAHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHG
HPTFSEVMYEAFADVLGEAIHNPPKRK

Sequences:

>Translated_587_residues
MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKTNMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEG
ESVEVSSPAASDVNVARTTEDLEAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVVKTLTGGVQGLLKANKVTIFNGL
GQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPGIDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVD
VTVIEMADRIIPAMDKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVPQMNGLENL
NLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAIHGNTTRKANLKYTPAAVYTHPEVAMVGLTE
EQAREQYGDVLIGKNSFTGNGRAIASNEAHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHG
HPTFSEVMYEAFADVLGEAIHNPPKRK
>Mature_586_residues
AVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKTNMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGE
SVEVSSPAASDVNVARTTEDLEAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEKS
EFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVVKTLTGGVQGLLKANKVTIFNGLG
QVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPGIDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDV
TVIEMADRIIPAMDKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVPQMNGLENLN
LEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAIHGNTTRKANLKYTPAAVYTHPEVAMVGLTEE
QAREQYGDVLIGKNSFTGNGRAIASNEAHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGH
PTFSEVMYEAFADVLGEAIHNPPKRK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=488, Percent_Identity=38.5245901639344, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI50301238, Length=456, Percent_Identity=26.7543859649123, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI33519430, Length=479, Percent_Identity=27.7661795407098, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI33519428, Length=479, Percent_Identity=27.7661795407098, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI33519426, Length=479, Percent_Identity=27.7661795407098, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI148277071, Length=479, Percent_Identity=27.7661795407098, Blast_Score=136, Evalue=7e-32,
Organism=Homo sapiens, GI148277065, Length=479, Percent_Identity=27.7661795407098, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI291045266, Length=437, Percent_Identity=27.2311212814645, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI22035672, Length=480, Percent_Identity=29.375, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI291045268, Length=434, Percent_Identity=24.6543778801843, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=36.7521367521368, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1789915, Length=429, Percent_Identity=28.4382284382284, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI87082354, Length=458, Percent_Identity=27.0742358078603, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=27.1334792122538, Blast_Score=158, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI32565766, Length=507, Percent_Identity=37.67258382643, Blast_Score=291, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=28.8100208768267, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71983419, Length=447, Percent_Identity=27.7404921700224, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=447, Percent_Identity=27.7404921700224, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71982272, Length=454, Percent_Identity=26.8722466960352, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=494, Percent_Identity=37.6518218623482, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=28.7526427061311, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=458, Percent_Identity=26.6375545851528, Blast_Score=153, Evalue=9e-38,
Organism=Drosophila melanogaster, GI21358499, Length=462, Percent_Identity=41.3419913419913, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24640549, Length=466, Percent_Identity=27.8969957081545, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24640553, Length=466, Percent_Identity=27.6824034334764, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640551, Length=466, Percent_Identity=27.8969957081545, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=27.6955602536998, Blast_Score=139, Evalue=7e-33,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 62459; Mature: 62328

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKTNMELEAEDSGVLLKIT
CEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEEEEE
RQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDLEAAGLEVPKAPAQAASAA
CCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEECCHHHHHCCCCCCCCCHHHHCCC
PKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEKSEFGGTCLNVGCIPTKTYLK
CCCCCCCCCEEEEEECCCCCCHHHEEECCCCCCEEEEEECCCCCCEEEEECCCCCHHHHC
NAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVVKTLTGGVQGLLKANKVTIFNGL
CCHHHCCEEEECCCCCEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECC
GQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPGIDSKLVLTSDDILDLREMPKSLAV
CCCCCCCEEEECCEEECCCEEEEECCCCEEEEECCCCCCEEEEECCCHHHHHHCCHHHHH
MGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKEVSLELQKILSKKGMKIKTSVGVS
HCCCEEEEEEEEEEECCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCHH
EIVEANNQLTLKLNNGEEVVAEKALLSIGRVPQMNGLENLNLEMDRNRIKVNDYQETSIP
HHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEECCCCCCCCC
GIYAPGDVNGTKMLAHAAYRMGEVAAENAIHGNTTRKANLKYTPAAVYTHPEVAMVGLTE
CEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCEEEEECCCEEEEECCH
EQAREQYGDVLIGKNSFTGNGRAIASNEAHGFVKVIADAKYHEILGVHIIGPAAAEMINE
HHHHHHHCCEEEECCCCCCCCEEEECCCCCCEEEEEECCCCCEEEEEEEECHHHHHHHHH
AATIMESELTVDELLLSIHGHPTFSEVMYEAFADVLGEAIHNPPKRK
HHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKTNMELEAEDSGVLLKIT
EEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEEEEE
RQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDLEAAGLEVPKAPAQAASAA
CCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEECCHHHHHCCCCCCCCCHHHHCCC
PKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEKSEFGGTCLNVGCIPTKTYLK
CCCCCCCCCEEEEEECCCCCCHHHEEECCCCCCEEEEEECCCCCCEEEEECCCCCHHHHC
NAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVVKTLTGGVQGLLKANKVTIFNGL
CCHHHCCEEEECCCCCEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECC
GQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPGIDSKLVLTSDDILDLREMPKSLAV
CCCCCCCEEEECCEEECCCEEEEECCCCEEEEECCCCCCEEEEECCCHHHHHHCCHHHHH
MGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKEVSLELQKILSKKGMKIKTSVGVS
HCCCEEEEEEEEEEECCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCHH
EIVEANNQLTLKLNNGEEVVAEKALLSIGRVPQMNGLENLNLEMDRNRIKVNDYQETSIP
HHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEECCCCCCCCC
GIYAPGDVNGTKMLAHAAYRMGEVAAENAIHGNTTRKANLKYTPAAVYTHPEVAMVGLTE
CEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCEEEEECCCEEEEECCH
EQAREQYGDVLIGKNSFTGNGRAIASNEAHGFVKVIADAKYHEILGVHIIGPAAAEMINE
HHHHHHHCCEEEECCCCCCCCEEEECCCCCCEEEEEECCCCCEEEEEEEECHHHHHHHHH
AATIMESELTVDELLLSIHGHPTFSEVMYEAFADVLGEAIHNPPKRK
HHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2832161; 2404760; 1880807 [H]