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The map label for this gene is sucB

Identifier: 21672570

GI number: 21672570

Start: 336668

End: 337849

Strand: Direct

Name: sucB

Synonym: BUsg293

Alternate gene names: 21672570

Gene position: 336668-337849 (Clockwise)

Preceding gene: 21672569

Following gene: 21672571

Centisome position: 52.49

GC content: 24.7

Gene sequence:

>1182_bases
ATGAATAGAATAAATATTCTTGTTCCTGATTTACCTGAATCTGTTAACGATGCAGTAGTTGTAAAATGGTATAAAAAAAT
AGGAGAACAAATTTCTTCTGAAGATAATATAGTAGATATTGAAACAGATAAAGTAATGCTAGAAGTGTCTGCTCCATGTA
ATGGAATATTAAATGAAATTTTAGAAAAAGAAGGTTCAATTGTTAAATCAAATCAAATATTAGGAAACATTGTTGAATCA
AAAAATATTGAAAGTAAAACAAAATCAAAACTGGAAAAAAGTAATAAATATTTTATAAAAGATAAAAATTTTAATATTTC
TTTTAAAGAAAAAATATACAATTTTCCACCTTCTATAAGACGTATAATAAGAATTAAAAAAAACAAAGAAATATTTAATG
AATTAAATTATATCAAAAATCAAGAAAACATAATTGAAGAAAAATTAAATGATCAATCTTTTTCTAATGAAAAAGAAAAA
AAAATTTATGAAAATAGAATAAAAATGACGCGATTGCGTCAAAAAATTGCTGAAAGATTATTAGAAACTAAAAATAATAC
AGCTATGCTTACTACCTTTAATGAAGTAAACATGCAACCAATAATATCATTACGAAAAAAATATGGCGAATTTTTTGAAA
AAAAACACGGTGTCCGAATCGGATTTATGCCTTTTTTTGTCAAAGCAGTAGTGGAATCCTTAAAAAAGTTTCCAGAAATA
AATGCCTCTATTGATAAAAATGATATTGTTTATTATAAAAATATTGATGTTAGTATTGCTGTTTCTACTCCGAGAGGAGT
AATAACACCAGTTTTAAGAAATGCAGATAATATGAGCATGGCTGATATAGAAAAAAAAATAAAAGAATTTTCTATTAAAG
GTATTGAAAATAAAATAAAAATTGAAGAATTAATTGGAGGAAATTTTACTATAACGAATGGCGGTATTTTCGGTTCTTTA
ATGTCTACACCTATTATTAATCCACCTCAATCTGCTATATTAGGCATGCATCTTATTAAAGAACGTCCTATGGCTATCAA
TGGAAAAGTAAAAATCCTTCCTATGATGTATTTAGCATTGTCTTACGATCATCGACTAATAGATGGAAAAGAATCTGTGA
GTTTTTTAGTAACTATAAAAAACATATTAGAAGACTTTAATCGAATTATAATTAATGTATAA

Upstream 100 bases:

>100_bases
CTGCATCACCTGCAGCTGGTCATATTCTAATTCATAGAAAAGAACAAGAAAAACTAATAAATAATGCATTCAATTTTAAA
ATAGCATAGAAGGACATAAA

Downstream 100 bases:

>100_bases
ATGTGTTTTTATGAAAAAAAACGTTTATTTTTTAGTGTATCTTAAAAGTACAAAAAATTCTTAATTATGAATTGTACTTT
TATATTCATAAAAATTAATT

Product: 2-oxoglutarate dehydrogenase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNRINILVPDLPESVNDAVVVKWYKKIGEQISSEDNIVDIETDKVMLEVSAPCNGILNEILEKEGSIVKSNQILGNIVES
KNIESKTKSKLEKSNKYFIKDKNFNISFKEKIYNFPPSIRRIIRIKKNKEIFNELNYIKNQENIIEEKLNDQSFSNEKEK
KIYENRIKMTRLRQKIAERLLETKNNTAMLTTFNEVNMQPIISLRKKYGEFFEKKHGVRIGFMPFFVKAVVESLKKFPEI
NASIDKNDIVYYKNIDVSIAVSTPRGVITPVLRNADNMSMADIEKKIKEFSIKGIENKIKIEELIGGNFTITNGGIFGSL
MSTPIINPPQSAILGMHLIKERPMAINGKVKILPMMYLALSYDHRLIDGKESVSFLVTIKNILEDFNRIIINV

Sequences:

>Translated_393_residues
MNRINILVPDLPESVNDAVVVKWYKKIGEQISSEDNIVDIETDKVMLEVSAPCNGILNEILEKEGSIVKSNQILGNIVES
KNIESKTKSKLEKSNKYFIKDKNFNISFKEKIYNFPPSIRRIIRIKKNKEIFNELNYIKNQENIIEEKLNDQSFSNEKEK
KIYENRIKMTRLRQKIAERLLETKNNTAMLTTFNEVNMQPIISLRKKYGEFFEKKHGVRIGFMPFFVKAVVESLKKFPEI
NASIDKNDIVYYKNIDVSIAVSTPRGVITPVLRNADNMSMADIEKKIKEFSIKGIENKIKIEELIGGNFTITNGGIFGSL
MSTPIINPPQSAILGMHLIKERPMAINGKVKILPMMYLALSYDHRLIDGKESVSFLVTIKNILEDFNRIIINV
>Mature_393_residues
MNRINILVPDLPESVNDAVVVKWYKKIGEQISSEDNIVDIETDKVMLEVSAPCNGILNEILEKEGSIVKSNQILGNIVES
KNIESKTKSKLEKSNKYFIKDKNFNISFKEKIYNFPPSIRRIIRIKKNKEIFNELNYIKNQENIIEEKLNDQSFSNEKEK
KIYENRIKMTRLRQKIAERLLETKNNTAMLTTFNEVNMQPIISLRKKYGEFFEKKHGVRIGFMPFFVKAVVESLKKFPEI
NASIDKNDIVYYKNIDVSIAVSTPRGVITPVLRNADNMSMADIEKKIKEFSIKGIENKIKIEELIGGNFTITNGGIFGSL
MSTPIINPPQSAILGMHLIKERPMAINGKVKILPMMYLALSYDHRLIDGKESVSFLVTIKNILEDFNRIIINV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=53.8793103448276, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI110671329, Length=407, Percent_Identity=24.5700245700246, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI31711992, Length=222, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI203098753, Length=230, Percent_Identity=28.695652173913, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI203098816, Length=230, Percent_Identity=28.695652173913, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=30.3571428571429, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786946, Length=407, Percent_Identity=54.2997542997543, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1786305, Length=207, Percent_Identity=31.4009661835749, Blast_Score=111, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25146366, Length=405, Percent_Identity=35.5555555555556, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17538894, Length=270, Percent_Identity=30.3703703703704, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17537937, Length=419, Percent_Identity=22.4343675417661, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17560088, Length=231, Percent_Identity=32.9004329004329, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=401, Percent_Identity=36.9077306733167, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6324258, Length=230, Percent_Identity=30.8695652173913, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645909, Length=222, Percent_Identity=54.5045045045045, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=33.0472103004292, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=33.0472103004292, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI18859875, Length=197, Percent_Identity=29.4416243654822, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ODO2_BUCAP (Q8K9N2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660637.1
- ProteinModelPortal:   Q8K9N2
- EnsemblBacteria:   EBBUCT00000000058
- GeneID:   1005497
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg293
- GeneTree:   EBGT00050000007942
- HOGENOM:   HBG630916
- OMA:   EINAYID
- ProtClustDB:   CLSK315790
- BioCyc:   BAPH198804:BUSG293-MONOMER
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR011053
- InterPro:   IPR006255
- Gene3D:   G3DSA:3.30.559.10
- TIGRFAMs:   TIGR01347

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; SSF51230 Hybrid_motif

EC number: =2.3.1.61

Molecular weight: Translated: 45154; Mature: 45154

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: ACT_SITE 364-364 ACT_SITE 368-368

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRINILVPDLPESVNDAVVVKWYKKIGEQISSEDNIVDIETDKVMLEVSAPCNGILNEI
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCHHHHHHHH
LEKEGSIVKSNQILGNIVESKNIESKTKSKLEKSNKYFIKDKNFNISFKEKIYNFPPSIR
HHHCCCEEECHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCEEHHHHHHCCCHHHH
RIIRIKKNKEIFNELNYIKNQENIIEEKLNDQSFSNEKEKKIYENRIKMTRLRQKIAERL
HHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LETKNNTAMLTTFNEVNMQPIISLRKKYGEFFEKKHGVRIGFMPFFVKAVVESLKKFPEI
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCC
NASIDKNDIVYYKNIDVSIAVSTPRGVITPVLRNADNMSMADIEKKIKEFSIKGIENKIK
CCCCCCCCEEEEECCEEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEE
IEELIGGNFTITNGGIFGSLMSTPIINPPQSAILGMHLIKERPMAINGKVKILPMMYLAL
HHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCEEHHHHHHHHH
SYDHRLIDGKESVSFLVTIKNILEDFNRIIINV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MNRINILVPDLPESVNDAVVVKWYKKIGEQISSEDNIVDIETDKVMLEVSAPCNGILNEI
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCHHHHHHHH
LEKEGSIVKSNQILGNIVESKNIESKTKSKLEKSNKYFIKDKNFNISFKEKIYNFPPSIR
HHHCCCEEECHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCEEHHHHHHCCCHHHH
RIIRIKKNKEIFNELNYIKNQENIIEEKLNDQSFSNEKEKKIYENRIKMTRLRQKIAERL
HHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LETKNNTAMLTTFNEVNMQPIISLRKKYGEFFEKKHGVRIGFMPFFVKAVVESLKKFPEI
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCC
NASIDKNDIVYYKNIDVSIAVSTPRGVITPVLRNADNMSMADIEKKIKEFSIKGIENKIK
CCCCCCCCEEEEECCEEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEE
IEELIGGNFTITNGGIFGSLMSTPIINPPQSAILGMHLIKERPMAINGKVKILPMMYLAL
HHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCEEHHHHHHHHH
SYDHRLIDGKESVSFLVTIKNILEDFNRIIINV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438