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The map label for this gene is ispG

Identifier: 21672556

GI number: 21672556

Start: 314386

End: 315489

Strand: Direct

Name: ispG

Synonym: BUsg276

Alternate gene names: 21672556

Gene position: 314386-315489 (Clockwise)

Preceding gene: 21672555

Following gene: 21672557

Centisome position: 49.01

GC content: 27.36

Gene sequence:

>1104_bases
ATGAATAAATATAAAAATATTAAAAGAAGAAAATCTAATCGTATTTATGTTGGTAATGTACCTATTGGAGATGGAGCACC
TATTTCTGTGCAGTCAATGACTAATACTCAAACTACAAATATAGAAGAAACAATAAAACAAATTATAAAATTGAAAAAAG
TAGGAGTAGATATTGTTCGAATTTCAGTTCCTACCCTAGAAGCTGCAGAGTCATTTAAAATAATTAAATTAAATGTAGAC
GTTCCATTAATTGCTGATATACATTTTGATTATAGATTAGCTATTAAATCAATAAAATATGGAGCTGATTGTTTAAGAAT
TAATCCAGGTAATATTGGCAACAAAAGAAGAATATTAGACATCGTTAATTGTGCAAAAGACAAAAATATACCTATTAGAA
TTGGTGTTAATGCAGGTTCATTAGAAAATGATTTATTAAAAAAATACAAATCACCAATTCCTGAAGCTTTAGTAGAATCA
GCTATCAGACATATTGAATATTTAGATAGTTTAAACTTTAATCAATTTAAAGTTAGTGTAAAGACATCTGATGTCTTTTC
AGCAATAGAAGCAAATGAAATTTTAGCAAAAAAAACTGTGCAACCAATACATATTGGGATAACGGAATCAGGAGCTTTAC
GCAATGGGATAGTCAAATCTTCTATTGGAATAACTTCTTTATTATTATCTGGTATTGGTGATACTTTAAGAATTTCACTT
GCTGCAGATCCAGTAGAAGAGGTAAAGGTAGGATATGATATTTTAAAAACTTTAGGAATTAGATTTAGGGGTGTTAATTT
TATTGCATGTCCGACTTGTTCTAGGCAAGAATTTAATGTAATTGATGTAGTAAATCAATTAGAAAAAAATTTGGAAGATC
TTTCAACTCCTATGAATGTTTCTATTATCGGTTGTATTGTTAATGGAATAGGAGAGGCTAAGGTATCTACATTAGGAATT
GTTGGAGGATCTAAAACAAGTGCATTATATAAAGATGGTATTCGTCAAAAAAACAAGTTAAAAAATCAAGAAATTATCAA
AGAATTAGAGATTAAAATTCGTAAAAAAGCAAAATCATTAGATAAATTAAAAAAAATTATTTAA

Upstream 100 bases:

>100_bases
AAGCCTATTAACGTTTAAACGTTTTTAATATTTTATCTAAAATACTAAAATACTCTTGTAATTTATAAAGACAATATTAA
GCATATATATATAACGAAAA

Downstream 100 bases:

>100_bases
CTACGATTAAAAAATAAACATAAGAAAAATAATAAGAGAAAATAGTGAATAAAAAAATTAATTCAGTAAGAGGAATGCAC
GATTATCTTCCTGAAGAATT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MNKYKNIKRRKSNRIYVGNVPIGDGAPISVQSMTNTQTTNIEETIKQIIKLKKVGVDIVRISVPTLEAAESFKIIKLNVD
VPLIADIHFDYRLAIKSIKYGADCLRINPGNIGNKRRILDIVNCAKDKNIPIRIGVNAGSLENDLLKKYKSPIPEALVES
AIRHIEYLDSLNFNQFKVSVKTSDVFSAIEANEILAKKTVQPIHIGITESGALRNGIVKSSIGITSLLLSGIGDTLRISL
AADPVEEVKVGYDILKTLGIRFRGVNFIACPTCSRQEFNVIDVVNQLEKNLEDLSTPMNVSIIGCIVNGIGEAKVSTLGI
VGGSKTSALYKDGIRQKNKLKNQEIIKELEIKIRKKAKSLDKLKKII

Sequences:

>Translated_367_residues
MNKYKNIKRRKSNRIYVGNVPIGDGAPISVQSMTNTQTTNIEETIKQIIKLKKVGVDIVRISVPTLEAAESFKIIKLNVD
VPLIADIHFDYRLAIKSIKYGADCLRINPGNIGNKRRILDIVNCAKDKNIPIRIGVNAGSLENDLLKKYKSPIPEALVES
AIRHIEYLDSLNFNQFKVSVKTSDVFSAIEANEILAKKTVQPIHIGITESGALRNGIVKSSIGITSLLLSGIGDTLRISL
AADPVEEVKVGYDILKTLGIRFRGVNFIACPTCSRQEFNVIDVVNQLEKNLEDLSTPMNVSIIGCIVNGIGEAKVSTLGI
VGGSKTSALYKDGIRQKNKLKNQEIIKELEIKIRKKAKSLDKLKKII
>Mature_367_residues
MNKYKNIKRRKSNRIYVGNVPIGDGAPISVQSMTNTQTTNIEETIKQIIKLKKVGVDIVRISVPTLEAAESFKIIKLNVD
VPLIADIHFDYRLAIKSIKYGADCLRINPGNIGNKRRILDIVNCAKDKNIPIRIGVNAGSLENDLLKKYKSPIPEALVES
AIRHIEYLDSLNFNQFKVSVKTSDVFSAIEANEILAKKTVQPIHIGITESGALRNGIVKSSIGITSLLLSGIGDTLRISL
AADPVEEVKVGYDILKTLGIRFRGVNFIACPTCSRQEFNVIDVVNQLEKNLEDLSTPMNVSIIGCIVNGIGEAKVSTLGI
VGGSKTSALYKDGIRQKNKLKNQEIIKELEIKIRKKAKSLDKLKKII

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=366, Percent_Identity=66.6666666666667, Blast_Score=520, Evalue=1e-149,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_BUCAP (Q8K9P4)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660623.1
- ProteinModelPortal:   Q8K9P4
- EnsemblBacteria:   EBBUCT00000000140
- GeneID:   1005480
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg276
- GeneTree:   EBGT00050000008113
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   BAPH198804:BUSG276-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40441; Mature: 40441

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKYKNIKRRKSNRIYVGNVPIGDGAPISVQSMTNTQTTNIEETIKQIIKLKKVGVDIVR
CCCHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEE
ISVPTLEAAESFKIIKLNVDVPLIADIHFDYRLAIKSIKYGADCLRINPGNIGNKRRILD
EECCCHHCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHH
IVNCAKDKNIPIRIGVNAGSLENDLLKKYKSPIPEALVESAIRHIEYLDSLNFNQFKVSV
HHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
KTSDVFSAIEANEILAKKTVQPIHIGITESGALRNGIVKSSIGITSLLLSGIGDTLRISL
EHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHCCCCEEEEEE
AADPVEEVKVGYDILKTLGIRFRGVNFIACPTCSRQEFNVIDVVNQLEKNLEDLSTPMNV
ECCCHHHHHHHHHHHHHHCCEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
SIIGCIVNGIGEAKVSTLGIVGGSKTSALYKDGIRQKNKLKNQEIIKELEIKIRKKAKSL
EEEEEEHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKLKKII
HHHHHCC
>Mature Secondary Structure
MNKYKNIKRRKSNRIYVGNVPIGDGAPISVQSMTNTQTTNIEETIKQIIKLKKVGVDIVR
CCCHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEE
ISVPTLEAAESFKIIKLNVDVPLIADIHFDYRLAIKSIKYGADCLRINPGNIGNKRRILD
EECCCHHCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHH
IVNCAKDKNIPIRIGVNAGSLENDLLKKYKSPIPEALVESAIRHIEYLDSLNFNQFKVSV
HHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
KTSDVFSAIEANEILAKKTVQPIHIGITESGALRNGIVKSSIGITSLLLSGIGDTLRISL
EHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHCCCCEEEEEE
AADPVEEVKVGYDILKTLGIRFRGVNFIACPTCSRQEFNVIDVVNQLEKNLEDLSTPMNV
ECCCHHHHHHHHHHHHHHCCEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
SIIGCIVNGIGEAKVSTLGIVGGSKTSALYKDGIRQKNKLKNQEIIKELEIKIRKKAKSL
EEEEEEHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKLKKII
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438