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The map label for this gene is yfgB

Identifier: 21672555

GI number: 21672555

Start: 313181

End: 314272

Strand: Direct

Name: yfgB

Synonym: BUsg275

Alternate gene names: 21672555

Gene position: 313181-314272 (Clockwise)

Preceding gene: 21672553

Following gene: 21672556

Centisome position: 48.82

GC content: 24.45

Gene sequence:

>1092_bases
ATGCATAAAAGTATTAATATAAAAAATATTTCTGATGATAAAATCAACTTATTAGATTTAAATCGTAAAGAAATAGAAAT
TTTTCTTCTTTCTCTTGGAGCAAAAAAATTTGTTACAGATCAACTTATGAAATGGATTTACAATCGTCATTGCAATAATT
TTAATTTAATGTCAAATCTTAAAAAAGATATCAGAAAAAAATTAAATGAACGATCTTATATATTTGCATCAAATTTTATA
GAAGAAAAAATATCTTATGATGGTACTGTAAAATGGATTACATCTATTGATAAACAAAAAATAGAAACTATTTATATTCC
TGAAAAAAAACGTGCTACTCTTTGTGTTTCTTCTCAAATTGGTTGTTCTTTAAAATGCAAGTTTTGTGCTACTGGGCAAC
AAGGTTTTAATAGAAATTTAAAAGTGTCAGAAATCATTTCGCAAATTTGGCAAGCAAATAAAATTTTAAAAGAAAAAAAA
AATAACAGTACAATTACTAACATAGTTTTTATGGGTATGGGCGAACCTTTATTAAATTTAAATAACGTTATTTCTGCAAT
AAAAATTATTTTAGATAAAAATGGATTTGGATTATCGAAACGTCGTATTACTCTCTCTACTTCAGGAATTGTACCAGCAT
TAAATAAACTTATTAAAAAGATTGATGTTAGTTTAGCTATATCTTTACATGCACCAAATGATTTTATTAGAAATTCAATT
ATGCCGATAAATATGAAATATAATATCAAATCTTTTTTAAACTCTGTTTCTAAATACTTAAAACATTCTCATGCAAATAG
AGGTGGTGTTACTGTAGAATATGTCATGCTGAGGGGTATTAATGATTTAAATGAACATGCTGAAGAATTAGGAAATATTT
TAAAAAAAATACCTAGTAAAATAAATCTTATTCCTTGGAATTTTTTTAAAAATGCAAACTTTATATGCAGTAGTAAGAAT
AGGATTAATATTTTTGCAAATATTTTAAGAAAAAAAGGGTTTAATACAACAATTAGAAAAAATAGAGGTCAAGATATTGG
CGCTGCATGCGGTCAATTAACTGGTGATATAGTTAATCGTATTAAAAATTAA

Upstream 100 bases:

>100_bases
ATCATAATTTATATATTTGTAATATTTTTTAATTAAACTAATTATTATAACAATTATTTATATATAAAATATTTGTGAAC
TAATTACTTAAGGCTATTAG

Downstream 100 bases:

>100_bases
TTATAAATTTTTTAAGCCTATTAACGTTTAAACGTTTTTAATATTTTATCTAAAATACTAAAATACTCTTGTAATTTATA
AAGACAATATTAAGCATATA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MHKSINIKNISDDKINLLDLNRKEIEIFLLSLGAKKFVTDQLMKWIYNRHCNNFNLMSNLKKDIRKKLNERSYIFASNFI
EEKISYDGTVKWITSIDKQKIETIYIPEKKRATLCVSSQIGCSLKCKFCATGQQGFNRNLKVSEIISQIWQANKILKEKK
NNSTITNIVFMGMGEPLLNLNNVISAIKIILDKNGFGLSKRRITLSTSGIVPALNKLIKKIDVSLAISLHAPNDFIRNSI
MPINMKYNIKSFLNSVSKYLKHSHANRGGVTVEYVMLRGINDLNEHAEELGNILKKIPSKINLIPWNFFKNANFICSSKN
RINIFANILRKKGFNTTIRKNRGQDIGAACGQLTGDIVNRIKN

Sequences:

>Translated_363_residues
MHKSINIKNISDDKINLLDLNRKEIEIFLLSLGAKKFVTDQLMKWIYNRHCNNFNLMSNLKKDIRKKLNERSYIFASNFI
EEKISYDGTVKWITSIDKQKIETIYIPEKKRATLCVSSQIGCSLKCKFCATGQQGFNRNLKVSEIISQIWQANKILKEKK
NNSTITNIVFMGMGEPLLNLNNVISAIKIILDKNGFGLSKRRITLSTSGIVPALNKLIKKIDVSLAISLHAPNDFIRNSI
MPINMKYNIKSFLNSVSKYLKHSHANRGGVTVEYVMLRGINDLNEHAEELGNILKKIPSKINLIPWNFFKNANFICSSKN
RINIFANILRKKGFNTTIRKNRGQDIGAACGQLTGDIVNRIKN
>Mature_363_residues
MHKSINIKNISDDKINLLDLNRKEIEIFLLSLGAKKFVTDQLMKWIYNRHCNNFNLMSNLKKDIRKKLNERSYIFASNFI
EEKISYDGTVKWITSIDKQKIETIYIPEKKRATLCVSSQIGCSLKCKFCATGQQGFNRNLKVSEIISQIWQANKILKEKK
NNSTITNIVFMGMGEPLLNLNNVISAIKIILDKNGFGLSKRRITLSTSGIVPALNKLIKKIDVSLAISLHAPNDFIRNSI
MPINMKYNIKSFLNSVSKYLKHSHANRGGVTVEYVMLRGINDLNEHAEELGNILKKIPSKINLIPWNFFKNANFICSSKN
RINIFANILRKKGFNTTIRKNRGQDIGAACGQLTGDIVNRIKN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=353, Percent_Identity=58.6402266288952, Blast_Score=443, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_BUCAP (Q8K9P5)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660622.1
- ProteinModelPortal:   Q8K9P5
- EnsemblBacteria:   EBBUCT00000000039
- GeneID:   1005479
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg275
- GeneTree:   EBGT00050000007926
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK315859
- BioCyc:   BAPH198804:BUSG275-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41366; Mature: 41366

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKSINIKNISDDKINLLDLNRKEIEIFLLSLGAKKFVTDQLMKWIYNRHCNNFNLMSNL
CCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHH
KKDIRKKLNERSYIFASNFIEEKISYDGTVKWITSIDKQKIETIYIPEKKRATLCVSSQI
HHHHHHHHCCCCEEEHHHHHHHHHCCCCEEHHHHHCCHHCCEEEECCCCCCEEEEEECCC
GCSLKCKFCATGQQGFNRNLKVSEIISQIWQANKILKEKKNNSTITNIVFMGMGEPLLNL
CCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHH
NNVISAIKIILDKNGFGLSKRRITLSTSGIVPALNKLIKKIDVSLAISLHAPNDFIRNSI
HHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCEEEEEEEECCHHHHHCCC
MPINMKYNIKSFLNSVSKYLKHSHANRGGVTVEYVMLRGINDLNEHAEELGNILKKIPSK
EEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEHEECCCCHHHHHHHHHHHHHHHCCCC
INLIPWNFFKNANFICSSKNRINIFANILRKKGFNTTIRKNRGQDIGAACGQLTGDIVNR
CEEECCHHHCCCCEEECCCCCHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHH
IKN
HCC
>Mature Secondary Structure
MHKSINIKNISDDKINLLDLNRKEIEIFLLSLGAKKFVTDQLMKWIYNRHCNNFNLMSNL
CCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHH
KKDIRKKLNERSYIFASNFIEEKISYDGTVKWITSIDKQKIETIYIPEKKRATLCVSSQI
HHHHHHHHCCCCEEEHHHHHHHHHCCCCEEHHHHHCCHHCCEEEECCCCCCEEEEEECCC
GCSLKCKFCATGQQGFNRNLKVSEIISQIWQANKILKEKKNNSTITNIVFMGMGEPLLNL
CCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHH
NNVISAIKIILDKNGFGLSKRRITLSTSGIVPALNKLIKKIDVSLAISLHAPNDFIRNSI
HHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCEEEEEEEECCHHHHHCCC
MPINMKYNIKSFLNSVSKYLKHSHANRGGVTVEYVMLRGINDLNEHAEELGNILKKIPSK
EEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEHEECCCCHHHHHHHHHHHHHHHCCCC
INLIPWNFFKNANFICSSKNRINIFANILRKKGFNTTIRKNRGQDIGAACGQLTGDIVNR
CEEECCHHHCCCCEEECCCCCHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHH
IKN
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438