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The map label for this gene is purB

Identifier: 21672534

GI number: 21672534

Start: 289173

End: 290543

Strand: Direct

Name: purB

Synonym: BUsg253

Alternate gene names: 21672534

Gene position: 289173-290543 (Clockwise)

Preceding gene: 21672533

Following gene: 21672535

Centisome position: 45.08

GC content: 25.75

Gene sequence:

>1371_bases
ATGGAATTAAATTCATTAACAGCTATTTCTCCAATTGATGGTCGATATTCTAATTCTACTAAGTTATTACGAAATATTTT
TAGTGAGTTTGGTTTTTTGAAATACCGTCTTTACGTAGAAATTCAATGGTTAAAAAAATTAATTAATATGCATCAAATAT
TAGAGATCAAAAAAGTAGAAAAAACAGATATATTATTTCTTGATAACATTTTTGAGACATTTAATGAAGAAGATGCAATA
TCTGTTAAAAATATAGAAAAAGAAACTAATCATGATGTAAAAGCGTTAGAATATTTTTTAAGAAAAAAACTTTCTCAATC
AAAAAAATTATCTCCATTTTTAGAATTTGTACATTTTTTATGCACTTCAGAAGATATAAATAACATAGCATACGCTTTAA
TGATCAAGAATGCTCGTGATGAAATTATTTTACCATTATGGAAAAAAATAATTAATTTTTTAAAAAACGCTTCTTTCCAA
TATAGAAACAATTCATTACTATCTTTAACTCATGGTCAACCGGCTACGCCATCTACTATGGGAAAGGAAATGGTTAATTT
TTACTATCGTATGCAACGTCAATATCACAAATTAAAAAAAATAGAAATATTAGGAAAAATAAATGGTACTACTGGAAACT
ATAACGCACATTTAGTAGCGTATCCGAAAGTTAATTGGCATGCAGTCAGCAAAGAATTTATAACATCACTTGGAATTTTT
TGGAATCCATATACTACACAAATAGAACCACATGATTATATTGCAGAATTGTTTGGATGTGTATCTCTTTTTAATAATAT
TTTAATTGATTCTAATCGCGATATTTGGGGATATATTTCTCTTAATTATTTCAAACAAAAGTTAATAGATCAAGAAATAG
GTTCTTCCATAATGCCTCATAAAATTAATCCTATCGATTTTGAAAATTCTGAAGGTAATTTAGGATTATCTAATGCTTTA
ATGAATCATATGATAAATAAATTACCGATTTCTAGATGGCAGCGTGATTTAAGTGATTCTACTGTTTTAAGAAATATAGG
AGTAGTATTTGCCTATTCTATAATTGCATATAATTCAGTTTTGTTGGGGACAAATAAATTAACAATTAATACATCACAGT
TGTTGAAAAATTTAGATAATAATTGGTCTGTTTTATCGGAAGCAATTCAAACTGTTATGCGTCGCTATACAATCGAAAAT
GCATATGAAAAATTAAAAAAATTAACTAGAGGGAAAAAAATAGAAAAAGTTGATATACATAAATTTATTGACAAATTAAA
TATTCCAAAAATAGAAAAAGAACGTTTAAAAAAGATATCTCCATCTAATTATATTGGTGCAGCTAGCCAAATTATTGATG
AAATAAAATAA

Upstream 100 bases:

>100_bases
AGATAATTACTTTTAATAAAATTTAAAGAAAAATATTGTATAATAGAAAATTCGAAAATATTTTTAATATTTTTATCTTT
ACAGCGTGAGAATATAAAAT

Downstream 100 bases:

>100_bases
AAGTATACTAGTATGCATATTTATCTTTCAATTTTAAGGAATTTTTTCCAGCGATGTTAGAAATACTAATTCTTTCTATT
ATACTGCTAATGGGATGTAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELNSLTAISPIDGRYSNSTKLLRNIFSEFGFLKYRLYVEIQWLKKLINMHQILEIKKVEKTDILFLDNIFETFNEEDAI
SVKNIEKETNHDVKALEYFLRKKLSQSKKLSPFLEFVHFLCTSEDINNIAYALMIKNARDEIILPLWKKIINFLKNASFQ
YRNNSLLSLTHGQPATPSTMGKEMVNFYYRMQRQYHKLKKIEILGKINGTTGNYNAHLVAYPKVNWHAVSKEFITSLGIF
WNPYTTQIEPHDYIAELFGCVSLFNNILIDSNRDIWGYISLNYFKQKLIDQEIGSSIMPHKINPIDFENSEGNLGLSNAL
MNHMINKLPISRWQRDLSDSTVLRNIGVVFAYSIIAYNSVLLGTNKLTINTSQLLKNLDNNWSVLSEAIQTVMRRYTIEN
AYEKLKKLTRGKKIEKVDIHKFIDKLNIPKIEKERLKKISPSNYIGAASQIIDEIK

Sequences:

>Translated_456_residues
MELNSLTAISPIDGRYSNSTKLLRNIFSEFGFLKYRLYVEIQWLKKLINMHQILEIKKVEKTDILFLDNIFETFNEEDAI
SVKNIEKETNHDVKALEYFLRKKLSQSKKLSPFLEFVHFLCTSEDINNIAYALMIKNARDEIILPLWKKIINFLKNASFQ
YRNNSLLSLTHGQPATPSTMGKEMVNFYYRMQRQYHKLKKIEILGKINGTTGNYNAHLVAYPKVNWHAVSKEFITSLGIF
WNPYTTQIEPHDYIAELFGCVSLFNNILIDSNRDIWGYISLNYFKQKLIDQEIGSSIMPHKINPIDFENSEGNLGLSNAL
MNHMINKLPISRWQRDLSDSTVLRNIGVVFAYSIIAYNSVLLGTNKLTINTSQLLKNLDNNWSVLSEAIQTVMRRYTIEN
AYEKLKKLTRGKKIEKVDIHKFIDKLNIPKIEKERLKKISPSNYIGAASQIIDEIK
>Mature_456_residues
MELNSLTAISPIDGRYSNSTKLLRNIFSEFGFLKYRLYVEIQWLKKLINMHQILEIKKVEKTDILFLDNIFETFNEEDAI
SVKNIEKETNHDVKALEYFLRKKLSQSKKLSPFLEFVHFLCTSEDINNIAYALMIKNARDEIILPLWKKIINFLKNASFQ
YRNNSLLSLTHGQPATPSTMGKEMVNFYYRMQRQYHKLKKIEILGKINGTTGNYNAHLVAYPKVNWHAVSKEFITSLGIF
WNPYTTQIEPHDYIAELFGCVSLFNNILIDSNRDIWGYISLNYFKQKLIDQEIGSSIMPHKINPIDFENSEGNLGLSNAL
MNHMINKLPISRWQRDLSDSTVLRNIGVVFAYSIIAYNSVLLGTNKLTINTSQLLKNLDNNWSVLSEAIQTVMRRYTIEN
AYEKLKKLTRGKKIEKVDIHKFIDKLNIPKIEKERLKKISPSNYIGAASQIIDEIK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=61.6228070175439, Blast_Score=625, Evalue=1e-180,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR8_BUCAP (Q8K9Q7)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660601.1
- ProteinModelPortal:   Q8K9Q7
- SMR:   Q8K9Q7
- EnsemblBacteria:   EBBUCT00000000193
- GeneID:   1005455
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg253
- GeneTree:   EBGT00050000007811
- HOGENOM:   HBG290028
- OMA:   KVSEFIH
- ProtClustDB:   PRK09285
- BioCyc:   BAPH198804:BUSG253-MONOMER
- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00928

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.2

Molecular weight: Translated: 53024; Mature: 53024

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: ACT_SITE 91-91 ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNSLTAISPIDGRYSNSTKLLRNIFSEFGFLKYRLYVEIQWLKKLINMHQILEIKKVE
CCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCC
KTDILFLDNIFETFNEEDAISVKNIEKETNHDVKALEYFLRKKLSQSKKLSPFLEFVHFL
HHHEEHHHHHHHHCCCCCCEEHHHCCHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
CTSEDINNIAYALMIKNARDEIILPLWKKIINFLKNASFQYRNNSLLSLTHGQPATPSTM
HCCCHHHHHEEEEEEECCCCCEEHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCCHHH
GKEMVNFYYRMQRQYHKLKKIEILGKINGTTGNYNAHLVAYPKVNWHAVSKEFITSLGIF
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCC
WNPYTTQIEPHDYIAELFGCVSLFNNILIDSNRDIWGYISLNYFKQKLIDQEIGSSIMPH
CCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCEEEEEEHHHHHHHHHHHHHCCCCCCC
KINPIDFENSEGNLGLSNALMNHMINKLPISRWQRDLSDSTVLRNIGVVFAYSIIAYNSV
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LLGTNKLTINTSQLLKNLDNNWSVLSEAIQTVMRRYTIENAYEKLKKLTRGKKIEKVDIH
EEECCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
KFIDKLNIPKIEKERLKKISPSNYIGAASQIIDEIK
HHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MELNSLTAISPIDGRYSNSTKLLRNIFSEFGFLKYRLYVEIQWLKKLINMHQILEIKKVE
CCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCC
KTDILFLDNIFETFNEEDAISVKNIEKETNHDVKALEYFLRKKLSQSKKLSPFLEFVHFL
HHHEEHHHHHHHHCCCCCCEEHHHCCHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
CTSEDINNIAYALMIKNARDEIILPLWKKIINFLKNASFQYRNNSLLSLTHGQPATPSTM
HCCCHHHHHEEEEEEECCCCCEEHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCCHHH
GKEMVNFYYRMQRQYHKLKKIEILGKINGTTGNYNAHLVAYPKVNWHAVSKEFITSLGIF
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCC
WNPYTTQIEPHDYIAELFGCVSLFNNILIDSNRDIWGYISLNYFKQKLIDQEIGSSIMPH
CCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCEEEEEEHHHHHHHHHHHHHCCCCCCC
KINPIDFENSEGNLGLSNALMNHMINKLPISRWQRDLSDSTVLRNIGVVFAYSIIAYNSV
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LLGTNKLTINTSQLLKNLDNNWSVLSEAIQTVMRRYTIENAYEKLKKLTRGKKIEKVDIH
EEECCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
KFIDKLNIPKIEKERLKKISPSNYIGAASQIIDEIK
HHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438