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The map label for this gene is tgt

Identifier: 21672415

GI number: 21672415

Start: 139148

End: 140254

Strand: Direct

Name: tgt

Synonym: BUsg125

Alternate gene names: 21672415

Gene position: 139148-140254 (Clockwise)

Preceding gene: 21672414

Following gene: 21672416

Centisome position: 21.69

GC content: 26.47

Gene sequence:

>1107_bases
ATGAAATTTCAGGTTATTAGTCAAGACAAAGAAGCCCGACATGGTATGTTTAACTTTAATGGAAATATTATCGAAACTCC
TGTTTTTATGCCAGTTGGAACATATGGAACAGTAAAGAGTCTTAATATAGAAGAAATTAAAAATACTGGTAGTAAAATAA
TTTTGTCTAATGCACTCCACTTATATTTAAGACCAGGTCAAGATATAATAAAACTACATGGTAGTTTGCATAATTTTATG
AATTGGAACGGTCCTATTTTAACTGATTCAGGTGGATTTCAAATTTTTAGTCTTTCAAACTTTTGTAGAATTAAAGAAGA
AGGAGTAATTTTTAAAAATCATATTAATGGTAAAAAATTTTTTTTAACTCCAGAATTATCAATGGAAATTCAAATAGATT
TGGGTTCTAATATTATTATGATTTTTGATGAATGTATTGCATATAATAAGGATTGGGAAAAAACAAAGTTTGCCATGGAA
AGATCATTAAACTGGTCTAGAAGAAGTCGTATACATTTTGATTTAAAGAAAAAAAACAAGAAAAATCTTTTATTTGGTAT
TATTCATGGTGGATCATATAAATCTTTACGTGATATTTCTCTTAAAGAATTAATAAAAATGGATTTTGATGGATATGCTT
TAGGTGGATTAGCTGTTGGTGAATCTAAATTAGAAATGCATGATTTATTAGATCATATTACTCCTCAAATACCTAAAAAA
AAACCAAGGTACTTGATGGGAGTAGGAAAACCTGAAGATTTAATTGAATCTGTTTATCGTGGAATAGATATGTTTGATTG
TGTTCTCCCCACAAGAAATGCTAGGAATGGACATTTGTTTGTAACAAATGGTATAATAAAAATCAGAAATGCCAAATATA
AAAAAGATTTGTCTGTATTAGACAAAAAATGTACTTGTTATACTTGTCAAAATTATAGCCGATCTTATTTACATCATTTA
GATTCTTGTAATGAAATCTTAGGAGCTCGTCTTAATACAATACATAATTTACATTATTATCAAACTTTGATGTTCAATAT
AAGAAATGCAATAAAACAAAAAAAGTTTGATGAATTTGTATCAAATTTTTATAATCAAAAAAAATAA

Upstream 100 bases:

>100_bases
TAACTTATAACACACATGCTCCATATGAAAAAATACTAACTTAATATAATCTTACCTTTTATTAAAAAGTTGAATTTTTT
ATAAAATTTGCGGAAAAAAT

Downstream 100 bases:

>100_bases
ATATATTTTTTTAATACATTAGGAAAACTATTTAATGAGTTTTTTTATTAAAGATGCTAATGCAGCAGTGAATCAAGCAT
TAGAGGGAAATTCTTATTCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKFQVISQDKEARHGMFNFNGNIIETPVFMPVGTYGTVKSLNIEEIKNTGSKIILSNALHLYLRPGQDIIKLHGSLHNFM
NWNGPILTDSGGFQIFSLSNFCRIKEEGVIFKNHINGKKFFLTPELSMEIQIDLGSNIIMIFDECIAYNKDWEKTKFAME
RSLNWSRRSRIHFDLKKKNKKNLLFGIIHGGSYKSLRDISLKELIKMDFDGYALGGLAVGESKLEMHDLLDHITPQIPKK
KPRYLMGVGKPEDLIESVYRGIDMFDCVLPTRNARNGHLFVTNGIIKIRNAKYKKDLSVLDKKCTCYTCQNYSRSYLHHL
DSCNEILGARLNTIHNLHYYQTLMFNIRNAIKQKKFDEFVSNFYNQKK

Sequences:

>Translated_368_residues
MKFQVISQDKEARHGMFNFNGNIIETPVFMPVGTYGTVKSLNIEEIKNTGSKIILSNALHLYLRPGQDIIKLHGSLHNFM
NWNGPILTDSGGFQIFSLSNFCRIKEEGVIFKNHINGKKFFLTPELSMEIQIDLGSNIIMIFDECIAYNKDWEKTKFAME
RSLNWSRRSRIHFDLKKKNKKNLLFGIIHGGSYKSLRDISLKELIKMDFDGYALGGLAVGESKLEMHDLLDHITPQIPKK
KPRYLMGVGKPEDLIESVYRGIDMFDCVLPTRNARNGHLFVTNGIIKIRNAKYKKDLSVLDKKCTCYTCQNYSRSYLHHL
DSCNEILGARLNTIHNLHYYQTLMFNIRNAIKQKKFDEFVSNFYNQKK
>Mature_368_residues
MKFQVISQDKEARHGMFNFNGNIIETPVFMPVGTYGTVKSLNIEEIKNTGSKIILSNALHLYLRPGQDIIKLHGSLHNFM
NWNGPILTDSGGFQIFSLSNFCRIKEEGVIFKNHINGKKFFLTPELSMEIQIDLGSNIIMIFDECIAYNKDWEKTKFAME
RSLNWSRRSRIHFDLKKKNKKNLLFGIIHGGSYKSLRDISLKELIKMDFDGYALGGLAVGESKLEMHDLLDHITPQIPKK
KPRYLMGVGKPEDLIESVYRGIDMFDCVLPTRNARNGHLFVTNGIIKIRNAKYKKDLSVLDKKCTCYTCQNYSRSYLHHL
DSCNEILGARLNTIHNLHYYQTLMFNIRNAIKQKKFDEFVSNFYNQKK

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=363, Percent_Identity=41.0468319559229, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI13375872, Length=229, Percent_Identity=27.0742358078603, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786607, Length=367, Percent_Identity=63.7602179836512, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17544680, Length=364, Percent_Identity=39.2857142857143, Blast_Score=269, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI25146502, Length=137, Percent_Identity=31.3868613138686, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19920510, Length=353, Percent_Identity=41.643059490085, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI21355311, Length=170, Percent_Identity=29.4117647058824, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_BUCAP (Q8KA09)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660482.1
- ProteinModelPortal:   Q8KA09
- SMR:   Q8KA09
- EnsemblBacteria:   EBBUCT00000000505
- GeneID:   1005942
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg125
- GeneTree:   EBGT00050000007786
- HOGENOM:   HBG629929
- OMA:   KAMLPEN
- ProtClustDB:   PRK00112
- BioCyc:   BAPH198804:BUSG125-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42579; Mature: 42579

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFQVISQDKEARHGMFNFNGNIIETPVFMPVGTYGTVKSLNIEEIKNTGSKIILSNALH
CEEEEECCCCHHHCCEEECCCCEEECCEEEECCCCCCEECCCHHHHHCCCCEEEEECCEE
LYLRPGQDIIKLHGSLHNFMNWNGPILTDSGGFQIFSLSNFCRIKEEGVIFKNHINGKKF
EEECCCHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCEEEECCCCEEEEECCCCCEE
FLTPELSMEIQIDLGSNIIMIFDECIAYNKDWEKTKFAMERSLNWSRRSRIHFDLKKKNK
EEECCCCEEEEEECCCCEEEEEEHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCC
KNLLFGIIHGGSYKSLRDISLKELIKMDFDGYALGGLAVGESKLEMHDLLDHITPQIPKK
CCEEEEEEECCCCCHHHHCCHHHHHHHCCCCEEECCEEECCHHHHHHHHHHHHCCCCCCC
KPRYLMGVGKPEDLIESVYRGIDMFDCVLPTRNARNGHLFVTNGIIKIRNAKYKKDLSVL
CCCEEEECCCHHHHHHHHHCCCCCEEHHCCCCCCCCCEEEEECCEEEECCCCHHHHHHHH
DKKCTCYTCQNYSRSYLHHLDSCNEILGARLNTIHNLHYYQTLMFNIRNAIKQKKFDEFV
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNFYNQKK
HHHHCCCC
>Mature Secondary Structure
MKFQVISQDKEARHGMFNFNGNIIETPVFMPVGTYGTVKSLNIEEIKNTGSKIILSNALH
CEEEEECCCCHHHCCEEECCCCEEECCEEEECCCCCCEECCCHHHHHCCCCEEEEECCEE
LYLRPGQDIIKLHGSLHNFMNWNGPILTDSGGFQIFSLSNFCRIKEEGVIFKNHINGKKF
EEECCCHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCEEEECCCCEEEEECCCCCEE
FLTPELSMEIQIDLGSNIIMIFDECIAYNKDWEKTKFAMERSLNWSRRSRIHFDLKKKNK
EEECCCCEEEEEECCCCEEEEEEHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCC
KNLLFGIIHGGSYKSLRDISLKELIKMDFDGYALGGLAVGESKLEMHDLLDHITPQIPKK
CCEEEEEEECCCCCHHHHCCHHHHHHHCCCCEEECCEEECCHHHHHHHHHHHHCCCCCCC
KPRYLMGVGKPEDLIESVYRGIDMFDCVLPTRNARNGHLFVTNGIIKIRNAKYKKDLSVL
CCCEEEECCCHHHHHHHHHCCCCCEEHHCCCCCCCCCEEEEECCEEEECCCCHHHHHHHH
DKKCTCYTCQNYSRSYLHHLDSCNEILGARLNTIHNLHYYQTLMFNIRNAIKQKKFDEFV
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNFYNQKK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438