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The map label for this gene is queA

Identifier: 21672414

GI number: 21672414

Start: 138018

End: 139091

Strand: Direct

Name: queA

Synonym: BUsg124

Alternate gene names: 21672414

Gene position: 138018-139091 (Clockwise)

Preceding gene: 21672413

Following gene: 21672415

Centisome position: 21.52

GC content: 24.21

Gene sequence:

>1074_bases
ATGCATCTTAGTAATTTTTATTTTAAAATACCAAAATCACTTATAGCTTTTTATCCATGTTTTATTCGAAGTCAATCTCG
TTTACTTATAATCAATGGTCACACAGGGAAAATAGCTCATAAATTTTTTTTTAATATTTTAAATGAAATTAATTCTGGTG
ATTTGATCATTTTTAATGATACTAAAGTAATTCCTGCTCGTTTATTTGGTTATAAAAAAAGTGGTGGTAGAGTTGAAGTT
TTATTAGAAAGAATATTAAATAAAAATAGTATTTTAGCCAGTATTAAATCTTCTAATGAAATTAAAATGAATTCTTATTT
GTTTTTTGGAAAAAAAAATAAAATTAAATCTTTTATAATTGGTTATAAAAATCCCTTTTACATAATTAAATTTTTGCATA
ATAAGAAATCAGTTATTGATATATTTAATAATATTGGACATATACCCTTACCTCCTTATATTAAAAGATTTAATGAAAAA
ATAGATTCAAGTTTATATCAAACAGTGTATAAAAAAAATTTAGGTTCTGTTGCTGCACCAACTGCTGGATTACATTTTGA
TTTACCTTTATTAGAAGCATTAAGTAAAAAAGGTGTTGATATAGATTTTTTAACATTACACATAGGAAGCGGAACATTTC
AACCTATTAGAACTGCAAAAATAGAAAAACATATTATGCATTCTGAATTGGTTGAAGTTTCATCAAATTTAATTCAAAAG
ATTAAATTATGTAAGAAAAAAGGCGGTCGTATAATTGCGGTTGGTACTACTACTTTGCGTGCATTAGAAAGTGCTTATCA
TTCTAATTCGTGGAATAATAAGAAAAACTATGTTAAAGATACAAATATTTTTATATATCCTGGTTATAAACATAATGTTG
TAGATGCTTTAATTACTAACTTTCATTTTCCTGAATCAACATTGATAATGCTAGTATGTTCTTTTTTAGGTTATAAAAAT
ACTATGAATGCTTATCATGAAGCAATTAAAAATAATTATCGTTTTTTTAGTTATGGGGATGCAATGTACATAACTTATAA
CACACATGCTCCATATGAAAAAATACTAACTTAA

Upstream 100 bases:

>100_bases
TTATTTAATGAAGGTTAAATATTAACGATATTTAATTTTTACTTGTAAAAAAAATCTATATATATTAGGTTTATACACTA
TTCTCTTAAAGAAATAAAAT

Downstream 100 bases:

>100_bases
TATAATCTTACCTTTTATTAAAAAGTTGAATTTTTTATAAAATTTGCGGAAAAAATATGAAATTTCAGGTTATTAGTCAA
GACAAAGAAGCCCGACATGG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MHLSNFYFKIPKSLIAFYPCFIRSQSRLLIINGHTGKIAHKFFFNILNEINSGDLIIFNDTKVIPARLFGYKKSGGRVEV
LLERILNKNSILASIKSSNEIKMNSYLFFGKKNKIKSFIIGYKNPFYIIKFLHNKKSVIDIFNNIGHIPLPPYIKRFNEK
IDSSLYQTVYKKNLGSVAAPTAGLHFDLPLLEALSKKGVDIDFLTLHIGSGTFQPIRTAKIEKHIMHSELVEVSSNLIQK
IKLCKKKGGRIIAVGTTTLRALESAYHSNSWNNKKNYVKDTNIFIYPGYKHNVVDALITNFHFPESTLIMLVCSFLGYKN
TMNAYHEAIKNNYRFFSYGDAMYITYNTHAPYEKILT

Sequences:

>Translated_357_residues
MHLSNFYFKIPKSLIAFYPCFIRSQSRLLIINGHTGKIAHKFFFNILNEINSGDLIIFNDTKVIPARLFGYKKSGGRVEV
LLERILNKNSILASIKSSNEIKMNSYLFFGKKNKIKSFIIGYKNPFYIIKFLHNKKSVIDIFNNIGHIPLPPYIKRFNEK
IDSSLYQTVYKKNLGSVAAPTAGLHFDLPLLEALSKKGVDIDFLTLHIGSGTFQPIRTAKIEKHIMHSELVEVSSNLIQK
IKLCKKKGGRIIAVGTTTLRALESAYHSNSWNNKKNYVKDTNIFIYPGYKHNVVDALITNFHFPESTLIMLVCSFLGYKN
TMNAYHEAIKNNYRFFSYGDAMYITYNTHAPYEKILT
>Mature_357_residues
MHLSNFYFKIPKSLIAFYPCFIRSQSRLLIINGHTGKIAHKFFFNILNEINSGDLIIFNDTKVIPARLFGYKKSGGRVEV
LLERILNKNSILASIKSSNEIKMNSYLFFGKKNKIKSFIIGYKNPFYIIKFLHNKKSVIDIFNNIGHIPLPPYIKRFNEK
IDSSLYQTVYKKNLGSVAAPTAGLHFDLPLLEALSKKGVDIDFLTLHIGSGTFQPIRTAKIEKHIMHSELVEVSSNLIQK
IKLCKKKGGRIIAVGTTTLRALESAYHSNSWNNKKNYVKDTNIFIYPGYKHNVVDALITNFHFPESTLIMLVCSFLGYKN
TMNAYHEAIKNNYRFFSYGDAMYITYNTHAPYEKILT

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=355, Percent_Identity=52.112676056338, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_BUCAP (Q8KA10)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660481.1
- ProteinModelPortal:   Q8KA10
- SMR:   Q8KA10
- EnsemblBacteria:   EBBUCT00000000462
- GeneID:   1005941
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg124
- GeneTree:   EBGT00050000008017
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   BAPH198804:BUSG124-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40918; Mature: 40918

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLSNFYFKIPKSLIAFYPCFIRSQSRLLIINGHTGKIAHKFFFNILNEINSGDLIIFND
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECC
TKVIPARLFGYKKSGGRVEVLLERILNKNSILASIKSSNEIKMNSYLFFGKKNKIKSFII
CCEEHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCCCEEEEE
GYKNPFYIIKFLHNKKSVIDIFNNIGHIPLPPYIKRFNEKIDSSLYQTVYKKNLGSVAAP
CCCCCEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TAGLHFDLPLLEALSKKGVDIDFLTLHIGSGTFQPIRTAKIEKHIMHSELVEVSSNLIQK
CCCCEECCHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IKLCKKKGGRIIAVGTTTLRALESAYHSNSWNNKKNYVKDTNIFIYPGYKHNVVDALITN
HHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCEECCEEEEECCCCHHHHHHHHHH
FHFPESTLIMLVCSFLGYKNTMNAYHEAIKNNYRFFSYGDAMYITYNTHAPYEKILT
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCHHHHCC
>Mature Secondary Structure
MHLSNFYFKIPKSLIAFYPCFIRSQSRLLIINGHTGKIAHKFFFNILNEINSGDLIIFND
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECC
TKVIPARLFGYKKSGGRVEVLLERILNKNSILASIKSSNEIKMNSYLFFGKKNKIKSFII
CCEEHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCCCEEEEE
GYKNPFYIIKFLHNKKSVIDIFNNIGHIPLPPYIKRFNEKIDSSLYQTVYKKNLGSVAAP
CCCCCEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TAGLHFDLPLLEALSKKGVDIDFLTLHIGSGTFQPIRTAKIEKHIMHSELVEVSSNLIQK
CCCCEECCHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IKLCKKKGGRIIAVGTTTLRALESAYHSNSWNNKKNYVKDTNIFIYPGYKHNVVDALITN
HHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCEECCEEEEECCCCHHHHHHHHHH
FHFPESTLIMLVCSFLGYKNTMNAYHEAIKNNYRFFSYGDAMYITYNTHAPYEKILT
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438