Click here to switch to the map view.

The map label for this gene is metG

Identifier: 21672394

GI number: 21672394

Start: 114350

End: 115987

Strand: Direct

Name: metG

Synonym: BUsg102

Alternate gene names: 21672394

Gene position: 114350-115987 (Clockwise)

Preceding gene: 21672393

Following gene: 21672396

Centisome position: 17.83

GC content: 24.36

Gene sequence:

>1638_bases
ATGTCTAATAAATTCAAAAAAATTTTAGTTACTTGTGCATTACCATACGCTAATGGTCCTATTCATATAGGTCATATGCT
TGAGCACATTCAAGCAGATATTTGGGTTCGTTATCAAAGAATGAGAAATAATGAAGTTTGGTTTATTTCTTCTGACGATG
CACATGGTACAGCTATTATGTTAAAATCTGAAAAATTAGGAATAACACCTATAAAATTAATTAAAAAAATTAAAGAAGAG
CATATAATAGATTTTTCAAATTTTAATATTTCACATGACAACTATCATAGTACGCATTGTGTAGAGAATTTATTTCTATT
AAGAAAAATATTTTTATCTTTAAGTGAACAGAAACTTATTAATGAAAAAAAAATTTTTCAATTTTATGATAATACAAAAA
AAATGTTTCTTCCAGATAGATTCATAAAAGGTACCTGTCCTTTTTGTTCTTCAAAAAATCAAAACGGAGACAACTGTGAA
ATATGCGGTTCAATTTACGAACCTACAGATTTGGTTAATCCTATTTCTGTAATTTCAAATAGTGTTCCTATTTTAAAAAA
TACGACACATTTATATTTTAATTTACCACTTTTTACTAATATGTTAAATAAATGGATACATTCTGGTATTTTAGAAAAAT
CAGTTGCTAAAAAAACGGAAGAATGGTTAAAATCAGGTTTAAAAGAGTGGGGTATTTCTCGAGATGCTCCATATTTTGGA
TTTAAAATTCCAAAATTTGATAAGAAATATTTTTATGTTTGGCTAGATGCGCCTGTTGGTTATATAAGTGCATTTAAAAA
TTTTTGTACTAAAAATAAAAAAGTAGATTTTAATGAATTTTGGAAGAAAGAATCAAAATGTGAATTGTATCATTTTATTG
GTAAAGATATTATTTATTTTCATACCCTGTTTTGGCCTGCAATATTAGAGGCTTCTTCATATCGAAAACCTAATGGTATT
TTTGTTCATGGCCATCTAACTATAAATGGATTAAAATTATCAAAATCTCGAGGTTTTTTAATTAAAGCGTCAGACTGGAT
TAAATGTTTTGATTCAGATAGTTTACGTTATTATTATGCAAGTAAATTAAGTAATAATATTAATGATATTGAAATTAATT
TAGAGGATTTTATCCAAAAAATTAATAGTGATATTGTCAATAAATTAGTTAATTTGGCTTCAAGAAATGCTAGTTTTATT
CATAAGTATTTTGATGGATATTTAGCTGATTCTTTGGGAGATTGCAATTTGTATCAAGATTTTATTGATATAAGTAAAAA
AATTGCTAATTTTTTTGAAAATCGTCAATTTAGTAGTGTAATTCGCGAATGCATGAAATTGTTAGATTTAGCAAATCAAT
ATATTAATCAAAGAGAACCCTGGAAAATAAAACAAGATAATTTTAATAAATTACATACAATTTGTACTGTAGGTATTAAT
TTATTCAGATTAGTTGTAATTTTTTTAAAACCAATAATACCAGATTTAGCTAAAAAAACAGAGTATTTTTTAATTTCAGA
TCTTACTTGGAAGGGTATTGATAAACCTCTTTTATCTCATAAAATAAAAAAATTTAAAAAATTATATAATAGAATTAATC
ATGATAAAATTTTACAATTATCTCTTTTATGTAAATAA

Upstream 100 bases:

>100_bases
AGAATAAATACCTTTATATATAGTAATTTTTAAAAATGAAAAAGTTTACGATGAATCATATTTTGTATATGATATTACCT
ATTTAATTAAGTAAAAAATT

Downstream 100 bases:

>100_bases
AATCTCATTTTTTTTATCATTTTACTAGATGATTTTAATAAAATAAAAAGAAATTTTTTCTATTAAAATGTACGTTGTTG
ATCCAAGATATTTTCCAATT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSNKFKKILVTCALPYANGPIHIGHMLEHIQADIWVRYQRMRNNEVWFISSDDAHGTAIMLKSEKLGITPIKLIKKIKEE
HIIDFSNFNISHDNYHSTHCVENLFLLRKIFLSLSEQKLINEKKIFQFYDNTKKMFLPDRFIKGTCPFCSSKNQNGDNCE
ICGSIYEPTDLVNPISVISNSVPILKNTTHLYFNLPLFTNMLNKWIHSGILEKSVAKKTEEWLKSGLKEWGISRDAPYFG
FKIPKFDKKYFYVWLDAPVGYISAFKNFCTKNKKVDFNEFWKKESKCELYHFIGKDIIYFHTLFWPAILEASSYRKPNGI
FVHGHLTINGLKLSKSRGFLIKASDWIKCFDSDSLRYYYASKLSNNINDIEINLEDFIQKINSDIVNKLVNLASRNASFI
HKYFDGYLADSLGDCNLYQDFIDISKKIANFFENRQFSSVIRECMKLLDLANQYINQREPWKIKQDNFNKLHTICTVGIN
LFRLVVIFLKPIIPDLAKKTEYFLISDLTWKGIDKPLLSHKIKKFKKLYNRINHDKILQLSLLCK

Sequences:

>Translated_545_residues
MSNKFKKILVTCALPYANGPIHIGHMLEHIQADIWVRYQRMRNNEVWFISSDDAHGTAIMLKSEKLGITPIKLIKKIKEE
HIIDFSNFNISHDNYHSTHCVENLFLLRKIFLSLSEQKLINEKKIFQFYDNTKKMFLPDRFIKGTCPFCSSKNQNGDNCE
ICGSIYEPTDLVNPISVISNSVPILKNTTHLYFNLPLFTNMLNKWIHSGILEKSVAKKTEEWLKSGLKEWGISRDAPYFG
FKIPKFDKKYFYVWLDAPVGYISAFKNFCTKNKKVDFNEFWKKESKCELYHFIGKDIIYFHTLFWPAILEASSYRKPNGI
FVHGHLTINGLKLSKSRGFLIKASDWIKCFDSDSLRYYYASKLSNNINDIEINLEDFIQKINSDIVNKLVNLASRNASFI
HKYFDGYLADSLGDCNLYQDFIDISKKIANFFENRQFSSVIRECMKLLDLANQYINQREPWKIKQDNFNKLHTICTVGIN
LFRLVVIFLKPIIPDLAKKTEYFLISDLTWKGIDKPLLSHKIKKFKKLYNRINHDKILQLSLLCK
>Mature_544_residues
SNKFKKILVTCALPYANGPIHIGHMLEHIQADIWVRYQRMRNNEVWFISSDDAHGTAIMLKSEKLGITPIKLIKKIKEEH
IIDFSNFNISHDNYHSTHCVENLFLLRKIFLSLSEQKLINEKKIFQFYDNTKKMFLPDRFIKGTCPFCSSKNQNGDNCEI
CGSIYEPTDLVNPISVISNSVPILKNTTHLYFNLPLFTNMLNKWIHSGILEKSVAKKTEEWLKSGLKEWGISRDAPYFGF
KIPKFDKKYFYVWLDAPVGYISAFKNFCTKNKKVDFNEFWKKESKCELYHFIGKDIIYFHTLFWPAILEASSYRKPNGIF
VHGHLTINGLKLSKSRGFLIKASDWIKCFDSDSLRYYYASKLSNNINDIEINLEDFIQKINSDIVNKLVNLASRNASFIH
KYFDGYLADSLGDCNLYQDFIDISKKIANFFENRQFSSVIRECMKLLDLANQYINQREPWKIKQDNFNKLHTICTVGINL
FRLVVIFLKPIIPDLAKKTEYFLISDLTWKGIDKPLLSHKIKKFKKLYNRINHDKILQLSLLCK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=559, Percent_Identity=26.8336314847943, Blast_Score=222, Evalue=7e-58,
Organism=Homo sapiens, GI24308436, Length=513, Percent_Identity=23.3918128654971, Blast_Score=149, Evalue=6e-36,
Organism=Escherichia coli, GI1788432, Length=543, Percent_Identity=57.4585635359116, Blast_Score=704, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=527, Percent_Identity=28.4629981024668, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI212645096, Length=513, Percent_Identity=23.1968810916179, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=271, Percent_Identity=28.0442804428044, Blast_Score=111, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6321704, Length=561, Percent_Identity=27.4509803921569, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321610, Length=539, Percent_Identity=22.8200371057514, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24655311, Length=559, Percent_Identity=27.7280858676207, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI21358483, Length=513, Percent_Identity=25.7309941520468, Blast_Score=139, Evalue=5e-33,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_BUCAP (Q9ZHD7)

Other databases:

- EMBL:   AE013218
- EMBL:   AF067228
- RefSeq:   NP_660461.1
- ProteinModelPortal:   Q9ZHD7
- SMR:   Q9ZHD7
- EnsemblBacteria:   EBBUCT00000000313
- GeneID:   1005919
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg102
- GeneTree:   EBGT00050000007903
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- ProtClustDB:   PRK12268
- BioCyc:   BAPH198804:BUSG102-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 63823; Mature: 63692

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKFKKILVTCALPYANGPIHIGHMLEHIQADIWVRYQRMRNNEVWFISSDDAHGTAIM
CCCHHHEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEE
LKSEKLGITPIKLIKKIKEEHIIDFSNFNISHDNYHSTHCVENLFLLRKIFLSLSEQKLI
EECCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NEKKIFQFYDNTKKMFLPDRFIKGTCPFCSSKNQNGDNCEICGSIYEPTDLVNPISVISN
HHHHHHHHHCCCCCEECCCHHHCCCCCCCCCCCCCCCCCEECCCCCCCHHHCCHHHHHCC
SVPILKNTTHLYFNLPLFTNMLNKWIHSGILEKSVAKKTEEWLKSGLKEWGISRDAPYFG
CCCCEECCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FKIPKFDKKYFYVWLDAPVGYISAFKNFCTKNKKVDFNEFWKKESKCELYHFIGKDIIYF
CCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHH
HTLFWPAILEASSYRKPNGIFVHGHLTINGLKLSKSRGFLIKASDWIKCFDSDSLRYYYA
HHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEECCCCEEEECCHHHHCCCCCCEEEEEH
SKLSNNINDIEINLEDFIQKINSDIVNKLVNLASRNASFIHKYFDGYLADSLGDCNLYQD
HHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHH
FIDISKKIANFFENRQFSSVIRECMKLLDLANQYINQREPWKIKQDNFNKLHTICTVGIN
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHH
LFRLVVIFLKPIIPDLAKKTEYFLISDLTWKGIDKPLLSHKIKKFKKLYNRINHDKILQL
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
SLLCK
EEECC
>Mature Secondary Structure 
SNKFKKILVTCALPYANGPIHIGHMLEHIQADIWVRYQRMRNNEVWFISSDDAHGTAIM
CCHHHEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEE
LKSEKLGITPIKLIKKIKEEHIIDFSNFNISHDNYHSTHCVENLFLLRKIFLSLSEQKLI
EECCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NEKKIFQFYDNTKKMFLPDRFIKGTCPFCSSKNQNGDNCEICGSIYEPTDLVNPISVISN
HHHHHHHHHCCCCCEECCCHHHCCCCCCCCCCCCCCCCCEECCCCCCCHHHCCHHHHHCC
SVPILKNTTHLYFNLPLFTNMLNKWIHSGILEKSVAKKTEEWLKSGLKEWGISRDAPYFG
CCCCEECCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FKIPKFDKKYFYVWLDAPVGYISAFKNFCTKNKKVDFNEFWKKESKCELYHFIGKDIIYF
CCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHH
HTLFWPAILEASSYRKPNGIFVHGHLTINGLKLSKSRGFLIKASDWIKCFDSDSLRYYYA
HHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEECCCCEEEECCHHHHCCCCCCEEEEEH
SKLSNNINDIEINLEDFIQKINSDIVNKLVNLASRNASFIHKYFDGYLADSLGDCNLYQD
HHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHH
FIDISKKIANFFENRQFSSVIRECMKLLDLANQYINQREPWKIKQDNFNKLHTICTVGIN
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHH
LFRLVVIFLKPIIPDLAKKTEYFLISDLTWKGIDKPLLSHKIKKFKKLYNRINHDKILQL
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
SLLCK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438; 9767718