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The map label for this gene is hisI

Identifier: 21672391

GI number: 21672391

Start: 111044

End: 111667

Strand: Direct

Name: hisI

Synonym: BUsg099

Alternate gene names: 21672391

Gene position: 111044-111667 (Clockwise)

Preceding gene: 21672390

Following gene: 21672392

Centisome position: 17.31

GC content: 23.24

Gene sequence:

>624_bases
ATGTTAAGTAAAAAAGAAAATTTATTGAAGCTTGATTGGATAAAAACAAATGGTCTAATTCCAGCAATAATACAAGATTT
TGCATCTAATTTAGTTTTAATGCATGGATATATGAATAAAGAAGCTTTTTTAAAAACTCAAAAAGAAGGTTTTGTTACTT
TTTATTCTCGTACTAAAAAGCGTTTATGGACTAAAGGAGAAGAATCAGGTAACTTACTAAAAGTTATAGATATTGTTACA
GATTGTGATTATGATACTATATTAATTATAGTTGAACCTCTTGGAAAAACATGTCATTTAAATAGAAAAAGTTGCTTTTT
TTTAAAAGAAAATACATTAAATTTTCTTTCTAAATTAGAAGATCTTATAGAAGATAGAAAAAATTTTAATACAGATAATT
CATATACTGCTAGATTATATAAATCTGGTACGAAACGTATTGCACAAAAAGTTGGTGAAGAAGCTATAGAAACAATATTA
GCAGCAATGAAAAACGATGGAGATGAGCTTATAAATGAATCTTCAGATTTAATTTATCACTTAATTGTTTTATTGCATGA
TCAAAATTTAAATTTTAATTTAATTATTGAAAACTTAAAAAAAAGAAAAACAGAAAAATTATAA

Upstream 100 bases:

>100_bases
ATGTAGACGGTGTTTTAGCTGCTTCCGTTTTTCATAAGAAAATCGTTAACATAAAAGATTTAAAATATTTTTTAATTCAA
AAAGGTTTGGAAATTAGAAT

Downstream 100 bases:

>100_bases
ATTTGTAATATGAAATTTTAATTTTAAGTAAGAGAAAATATTTCTGTAAGTCATTATTTGTTGTTTTTTTAAAAATATTG
TTTTGAACAATATTTAACTT

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MLSKKENLLKLDWIKTNGLIPAIIQDFASNLVLMHGYMNKEAFLKTQKEGFVTFYSRTKKRLWTKGEESGNLLKVIDIVT
DCDYDTILIIVEPLGKTCHLNRKSCFFLKENTLNFLSKLEDLIEDRKNFNTDNSYTARLYKSGTKRIAQKVGEEAIETIL
AAMKNDGDELINESSDLIYHLIVLLHDQNLNFNLIIENLKKRKTEKL

Sequences:

>Translated_207_residues
MLSKKENLLKLDWIKTNGLIPAIIQDFASNLVLMHGYMNKEAFLKTQKEGFVTFYSRTKKRLWTKGEESGNLLKVIDIVT
DCDYDTILIIVEPLGKTCHLNRKSCFFLKENTLNFLSKLEDLIEDRKNFNTDNSYTARLYKSGTKRIAQKVGEEAIETIL
AAMKNDGDELINESSDLIYHLIVLLHDQNLNFNLIIENLKKRKTEKL
>Mature_207_residues
MLSKKENLLKLDWIKTNGLIPAIIQDFASNLVLMHGYMNKEAFLKTQKEGFVTFYSRTKKRLWTKGEESGNLLKVIDIVT
DCDYDTILIIVEPLGKTCHLNRKSCFFLKENTLNFLSKLEDLIEDRKNFNTDNSYTARLYKSGTKRIAQKVGEEAIETIL
AAMKNDGDELINESSDLIYHLIVLLHDQNLNFNLIIENLKKRKTEKL

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=202, Percent_Identity=55.9405940594059, Blast_Score=232, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6319819, Length=167, Percent_Identity=33.5329341317365, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS2_BUCAP (Q9ZHE0)

Other databases:

- EMBL:   AF067228
- EMBL:   AE013218
- RefSeq:   NP_660458.1
- ProteinModelPortal:   Q9ZHE0
- EnsemblBacteria:   EBBUCT00000000234
- GeneID:   1005916
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg099
- GeneTree:   EBGT00050000008126
- HOGENOM:   HBG294308
- OMA:   VMACKDD
- ProtClustDB:   PRK02759
- BioCyc:   BAPH198804:BUSG099-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01019
- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610
- TIGRFAMs:   TIGR03188

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH

EC number: =3.5.4.19; =3.6.1.31

Molecular weight: Translated: 23949; Mature: 23949

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKKENLLKLDWIKTNGLIPAIIQDFASNLVLMHGYMNKEAFLKTQKEGFVTFYSRTKK
CCCCCCCCEEEEEEECCCCHHHHHHHHHHCEEEEECCCCHHHHHHHHCCCHHHHHHHHHH
RLWTKGEESGNLLKVIDIVTDCDYDTILIIVEPLGKTCHLNRKSCFFLKENTLNFLSKLE
HHCCCCCCCCCEEEEEEHHHCCCCCEEEEEEECCCCEEECCCCEEEEEHHHHHHHHHHHH
DLIEDRKNFNTDNSYTARLYKSGTKRIAQKVGEEAIETILAAMKNDGDELINESSDLIYH
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHH
LIVLLHDQNLNFNLIIENLKKRKTEKL
HHHHHCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLSKKENLLKLDWIKTNGLIPAIIQDFASNLVLMHGYMNKEAFLKTQKEGFVTFYSRTKK
CCCCCCCCEEEEEEECCCCHHHHHHHHHHCEEEEECCCCHHHHHHHHCCCHHHHHHHHHH
RLWTKGEESGNLLKVIDIVTDCDYDTILIIVEPLGKTCHLNRKSCFFLKENTLNFLSKLE
HHCCCCCCCCCEEEEEEHHHCCCCCEEEEEEECCCCEEECCCCEEEEEHHHHHHHHHHHH
DLIEDRKNFNTDNSYTARLYKSGTKRIAQKVGEEAIETILAAMKNDGDELINESSDLIYH
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHH
LIVLLHDQNLNFNLIIENLKKRKTEKL
HHHHHCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9767718; 12089438