Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 21672390

GI number: 21672390

Start: 110274

End: 111050

Strand: Direct

Name: hisF

Synonym: BUsg098

Alternate gene names: 21672390

Gene position: 110274-111050 (Clockwise)

Preceding gene: 21672389

Following gene: 21672391

Centisome position: 17.19

GC content: 30.63

Gene sequence:

>777_bases
ATGCTGGCAAAGCGAATAATTGCATGTTTAGATGTTAACAATGGAGTTGTAGTAAAAGGCATTCAATTTAAAAACCATGA
AATTGTAGGTGATATTATATCTTTGTCTAAACGTTATACAAAAGAAGGTATAGATGAATTAGTATTTTATGACATAACAG
CTTCTACTAATAATAAGCTAGTTGATAGAAGTTGGATAAAGAAAGTAGCAGAAATAATTAATATTCCATTTTGCGTAGCC
GGAGGTATTAAAAGTGTAGAAGATGCACAGAATATTCTATCTTTTGGTGCAGATAAAATATCAATTAATTCTTCAGCATT
AATAGATCCTGATTTAATAACTAAAATTGCTAATCGTTTTGGTGTACAATGTACAGTTGTTGGCATTGATTCTTGGTTCG
ACTCAGACAAGAAAAAATACATGGTCCAACAATATACAGGTGACGCTAATCGTACTTATCAAACTGATTGGGAAACAATA
GATTGGGTTGAAAAAGTGCAAAAAAAAGGTGCTGGAGAAATTGTTTTAAACATGATGAATCAAGATGGATTACAAAATGG
ATATGATTTATCACAGTTAAATGAAATAAGAAAAAAATGTAAAGTACCTTTAATTGCATCAGGGGGGGCAGGGTGCGCAG
ATCATTTTTATCAAGCATTCGATCATGCTAATGTAGACGGTGTTTTAGCTGCTTCCGTTTTTCATAAGAAAATCGTTAAC
ATAAAAGATTTAAAATATTTTTTAATTCAAAAAGGTTTGGAAATTAGAATATGTTAA

Upstream 100 bases:

>100_bases
GATTAAAAGATCTTCTATCTTTAAAAAAAACAGGTGTTAAAAGTGTTATTATTGGTCGTAGTTTGTTAGAAAATAAGTTT
ACAATAGAAGAGGCATTAAA

Downstream 100 bases:

>100_bases
GTAAAAAAGAAAATTTATTGAAGCTTGATTGGATAAAAACAAATGGTCTAATTCCAGCAATAATACAAGATTTTGCATCT
AATTTAGTTTTAATGCATGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIACLDVNNGVVVKGIQFKNHEIVGDIISLSKRYTKEGIDELVFYDITASTNNKLVDRSWIKKVAEIINIPFCVA
GGIKSVEDAQNILSFGADKISINSSALIDPDLITKIANRFGVQCTVVGIDSWFDSDKKKYMVQQYTGDANRTYQTDWETI
DWVEKVQKKGAGEIVLNMMNQDGLQNGYDLSQLNEIRKKCKVPLIASGGAGCADHFYQAFDHANVDGVLAASVFHKKIVN
IKDLKYFLIQKGLEIRIC

Sequences:

>Translated_258_residues
MLAKRIIACLDVNNGVVVKGIQFKNHEIVGDIISLSKRYTKEGIDELVFYDITASTNNKLVDRSWIKKVAEIINIPFCVA
GGIKSVEDAQNILSFGADKISINSSALIDPDLITKIANRFGVQCTVVGIDSWFDSDKKKYMVQQYTGDANRTYQTDWETI
DWVEKVQKKGAGEIVLNMMNQDGLQNGYDLSQLNEIRKKCKVPLIASGGAGCADHFYQAFDHANVDGVLAASVFHKKIVN
IKDLKYFLIQKGLEIRIC
>Mature_258_residues
MLAKRIIACLDVNNGVVVKGIQFKNHEIVGDIISLSKRYTKEGIDELVFYDITASTNNKLVDRSWIKKVAEIINIPFCVA
GGIKSVEDAQNILSFGADKISINSSALIDPDLITKIANRFGVQCTVVGIDSWFDSDKKKYMVQQYTGDANRTYQTDWETI
DWVEKVQKKGAGEIVLNMMNQDGLQNGYDLSQLNEIRKKCKVPLIASGGAGCADHFYQAFDHANVDGVLAASVFHKKIVN
IKDLKYFLIQKGLEIRIC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=69.3798449612403, Blast_Score=393, Evalue=1e-111,
Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=23.4567901234568, Blast_Score=62, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=29.2993630573248, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_BUCAP (Q9ZHE1)

Other databases:

- EMBL:   AF067228
- EMBL:   AE013218
- RefSeq:   NP_660457.1
- ProteinModelPortal:   Q9ZHE1
- SMR:   Q9ZHE1
- EnsemblBacteria:   EBBUCT00000000223
- GeneID:   1005915
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg098
- GeneTree:   EBGT00050000007994
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- ProtClustDB:   PRK02083
- BioCyc:   BAPH198804:BUSG098-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28771; Mature: 28771

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIACLDVNNGVVVKGIQFKNHEIVGDIISLSKRYTKEGIDELVFYDITASTNNKL
CCHHHEEHEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCCH
VDRSWIKKVAEIINIPFCVAGGIKSVEDAQNILSFGADKISINSSALIDPDLITKIANRF
HHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCTVVGIDSWFDSDKKKYMVQQYTGDANRTYQTDWETIDWVEKVQKKGAGEIVLNMMN
CCEEEEEEECCHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHH
QDGLQNGYDLSQLNEIRKKCKVPLIASGGAGCADHFYQAFDHANVDGVLAASVFHKKIVN
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
IKDLKYFLIQKGLEIRIC
HHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIACLDVNNGVVVKGIQFKNHEIVGDIISLSKRYTKEGIDELVFYDITASTNNKL
CCHHHEEHEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCCH
VDRSWIKKVAEIINIPFCVAGGIKSVEDAQNILSFGADKISINSSALIDPDLITKIANRF
HHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCTVVGIDSWFDSDKKKYMVQQYTGDANRTYQTDWETIDWVEKVQKKGAGEIVLNMMN
CCEEEEEEECCHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHH
QDGLQNGYDLSQLNEIRKKCKVPLIASGGAGCADHFYQAFDHANVDGVLAASVFHKKIVN
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
IKDLKYFLIQKGLEIRIC
HHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9767718; 12089438