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The map label for this gene is cca

Identifier: 21672351

GI number: 21672351

Start: 66445

End: 67683

Strand: Direct

Name: cca

Synonym: BUsg058

Alternate gene names: 21672351

Gene position: 66445-67683 (Clockwise)

Preceding gene: 21672348

Following gene: 30410812

Centisome position: 10.36

GC content: 24.86

Gene sequence:

>1239_bases
ATGAAAGTATATTTAGTAGGAGGGGCTGTTCGTGACAGTTTATTAAACTTACCTATTAAAGATAGAGATTGGGTAATAGT
AGGAGGTACAAAGGAAATATTATTAAAAAAAAATTTTCAACAAGTAGGAAAAGATTTTCCTGTTTTTTTACATCCAGAAA
CACATGAAGAATATTCATTAGCAAGAAAAGAAAGAAAATCTGGTAGAGGATATACCGGTTTTCATACTGAATTTAGTTCT
GATGTTACTCTAGAAGAAGACTTAATTAGACGTGATTTGACAATTAATGCTATTGCTCAAGATGAAAACGGTAATTATAT
TGATCCTTTTCAAGGTAGAAAAGATCTAAATCTTAGATTATTGCGTCATGTTTCAGAGTCTTTTACGGAAGATCCATTAC
GTATTTTACGTACAGCAAGGTTTGCAGCTAATTTGATGCATTTAGGTTTTCATATTGATAAAGATACTATGATTTTAATG
TGTAAAATGGTAAAAAAAAATGAACTTTTATATTTAACTAAAAATAGAATTTGGAATGAAACTGAAAAAGCTTTTAAAAC
TCTAAATCCTCATGTTTATTTTCAAATTTTACATGCCTGTAAAGCATTAAATTTTATTTTTCCAGAAATTTTTTTTCTAT
ATGAAAGAAGAACATTTTTTTCTATACTTTTTACAAATTTTTACAGTATTTCTTTTCTTTCAATAGGTTTATCTAAGATA
TCTACTTTAACTAAAGATGTTTCTATACGATTTTCTTATCTATGCCAATTTTTGTCTGAAAAAATTGATTTTTCTTCTAC
GAAAGAAAATTATGATGATGATTCTGCTAAACTTGTAAAGAAATTATGTAAGCGTTGTAACATTCCATCACACATTGAAG
AATTAGCTGTTTTAAATACTGGTTTTTGTGTATTTTTGAGTTCAATTCATTATCAATCTTCTAGTAACATTATCAAGATG
TTTTCAAAAATTGATGCTTGGCGTAAACCAGATCGAATTTATAAATTATCCTTTTTATGTGATTTTAATTTATTTTATCA
TCAAAATAAAATAGATAATTCTAAGTTAAAAACTGGTTTTTTAAAAAAATGTTTTTTTATTATTAAAGATATTTCTGTTA
AAAAAATTTTAAATCAAGGTTTCAAAGGATATCAGATTAAAAATGAATTGAATAAATTAAGAGTTAACAAACTTAAATTT
TGGCGTATGAAAAAAAAATGTTTTTTTTTTAAAGAGTAA

Upstream 100 bases:

>100_bases
TTACAAGATTTTATTTTATAAAATTATAAATAATTATTTAATATATTTTATTTTTTCTATTATAGAATAGAATTTTACTT
TTTAGTATGAGAAAATTTTT

Downstream 100 bases:

>100_bases
ATTTTTTTATATAAAATAAATTATTCCTGCGATGAGAAAGCGATAAATTCCAAAAAAAGTCAATGATACCTTATTAAGTA
TAGTAATAAATTTTTTTATG

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKVYLVGGAVRDSLLNLPIKDRDWVIVGGTKEILLKKNFQQVGKDFPVFLHPETHEEYSLARKERKSGRGYTGFHTEFSS
DVTLEEDLIRRDLTINAIAQDENGNYIDPFQGRKDLNLRLLRHVSESFTEDPLRILRTARFAANLMHLGFHIDKDTMILM
CKMVKKNELLYLTKNRIWNETEKAFKTLNPHVYFQILHACKALNFIFPEIFFLYERRTFFSILFTNFYSISFLSIGLSKI
STLTKDVSIRFSYLCQFLSEKIDFSSTKENYDDDSAKLVKKLCKRCNIPSHIEELAVLNTGFCVFLSSIHYQSSSNIIKM
FSKIDAWRKPDRIYKLSFLCDFNLFYHQNKIDNSKLKTGFLKKCFFIIKDISVKKILNQGFKGYQIKNELNKLRVNKLKF
WRMKKKCFFFKE

Sequences:

>Translated_412_residues
MKVYLVGGAVRDSLLNLPIKDRDWVIVGGTKEILLKKNFQQVGKDFPVFLHPETHEEYSLARKERKSGRGYTGFHTEFSS
DVTLEEDLIRRDLTINAIAQDENGNYIDPFQGRKDLNLRLLRHVSESFTEDPLRILRTARFAANLMHLGFHIDKDTMILM
CKMVKKNELLYLTKNRIWNETEKAFKTLNPHVYFQILHACKALNFIFPEIFFLYERRTFFSILFTNFYSISFLSIGLSKI
STLTKDVSIRFSYLCQFLSEKIDFSSTKENYDDDSAKLVKKLCKRCNIPSHIEELAVLNTGFCVFLSSIHYQSSSNIIKM
FSKIDAWRKPDRIYKLSFLCDFNLFYHQNKIDNSKLKTGFLKKCFFIIKDISVKKILNQGFKGYQIKNELNKLRVNKLKF
WRMKKKCFFFKE
>Mature_412_residues
MKVYLVGGAVRDSLLNLPIKDRDWVIVGGTKEILLKKNFQQVGKDFPVFLHPETHEEYSLARKERKSGRGYTGFHTEFSS
DVTLEEDLIRRDLTINAIAQDENGNYIDPFQGRKDLNLRLLRHVSESFTEDPLRILRTARFAANLMHLGFHIDKDTMILM
CKMVKKNELLYLTKNRIWNETEKAFKTLNPHVYFQILHACKALNFIFPEIFFLYERRTFFSILFTNFYSISFLSIGLSKI
STLTKDVSIRFSYLCQFLSEKIDFSSTKENYDDDSAKLVKKLCKRCNIPSHIEELAVLNTGFCVFLSSIHYQSSSNIIKM
FSKIDAWRKPDRIYKLSFLCDFNLFYHQNKIDNSKLKTGFLKKCFFIIKDISVKKILNQGFKGYQIKNELNKLRVNKLKF
WRMKKKCFFFKE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=414, Percent_Identity=43.9613526570048, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI87081691, Length=172, Percent_Identity=33.7209302325581, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281360112, Length=225, Percent_Identity=28.8888888888889, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21357337, Length=225, Percent_Identity=28.8888888888889, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_BUCAP (Q8KA53)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660418.1
- ProteinModelPortal:   Q8KA53
- SMR:   Q8KA53
- EnsemblBacteria:   EBBUCT00000000291
- GeneID:   1005874
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg058
- GeneTree:   EBGT00050000008009
- HOGENOM:   HBG635063
- OMA:   QVGKGFP
- ProtClustDB:   PRK13298
- BioCyc:   BAPH198804:BUSG058-MONOMER
- HAMAP:   MF_01262
- InterPro:   IPR012006
- InterPro:   IPR002646
- PIRSF:   PIRSF000813

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 48583; Mature: 48583

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYLVGGAVRDSLLNLPIKDRDWVIVGGTKEILLKKNFQQVGKDFPVFLHPETHEEYSL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHH
ARKERKSGRGYTGFHTEFSSDVTLEEDLIRRDLTINAIAQDENGNYIDPFQGRKDLNLRL
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHH
LRHVSESFTEDPLRILRTARFAANLMHLGFHIDKDTMILMCKMVKKNELLYLTKNRIWNE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHCCCCEEEEECCCCCCH
TEKAFKTLNPHVYFQILHACKALNFIFPEIFFLYERRTFFSILFTNFYSISFLSIGLSKI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLTKDVSIRFSYLCQFLSEKIDFSSTKENYDDDSAKLVKKLCKRCNIPSHIEELAVLNT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCC
GFCVFLSSIHYQSSSNIIKMFSKIDAWRKPDRIYKLSFLCDFNLFYHQNKIDNSKLKTGF
CHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECHHHEECCCCCCHHHHHHH
LKKCFFIIKDISVKKILNQGFKGYQIKNELNKLRVNKLKFWRMKKKCFFFKE
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVYLVGGAVRDSLLNLPIKDRDWVIVGGTKEILLKKNFQQVGKDFPVFLHPETHEEYSL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHH
ARKERKSGRGYTGFHTEFSSDVTLEEDLIRRDLTINAIAQDENGNYIDPFQGRKDLNLRL
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHH
LRHVSESFTEDPLRILRTARFAANLMHLGFHIDKDTMILMCKMVKKNELLYLTKNRIWNE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHCCCCEEEEECCCCCCH
TEKAFKTLNPHVYFQILHACKALNFIFPEIFFLYERRTFFSILFTNFYSISFLSIGLSKI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLTKDVSIRFSYLCQFLSEKIDFSSTKENYDDDSAKLVKKLCKRCNIPSHIEELAVLNT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCC
GFCVFLSSIHYQSSSNIIKMFSKIDAWRKPDRIYKLSFLCDFNLFYHQNKIDNSKLKTGF
CHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECHHHEECCCCCCHHHHHHH
LKKCFFIIKDISVKKILNQGFKGYQIKNELNKLRVNKLKFWRMKKKCFFFKE
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438