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The map label for this gene is rpoH

Identifier: 21672319

GI number: 21672319

Start: 25214

End: 26071

Strand: Direct

Name: rpoH

Synonym: BUsg026

Alternate gene names: 21672319

Gene position: 25214-26071 (Clockwise)

Preceding gene: 21672318

Following gene: 21672322

Centisome position: 3.93

GC content: 29.6

Gene sequence:

>858_bases
ATGACCAATAAAGTACAGATTTTATCTGTAACAGCACTAGGTAATTTAGATGCTTATATTCGAATAGCTAATCTATGGCC
AATGTTGTCGATTAAAGAAGAAAAATTATTAACTAAACGTTTACGCTATCACGATGATTTAGATGCTGCAAAAACTTTAA
TTCTTTCTCATCTTCGTTTTGTTATTCATATTTCACGTAATTATTCGGGATACGGTTTACTCCAAGCTGATTTAATACAG
GAAGGTAATATAGGTTTAATGAAAGCAGTACGTAGATTCAATCCAGACATAGGAGTACGTCTTGTTTCTTTTGCTGTACA
TTGGATTAAATCAGAAATACATGAGTATGTATTGCGTAATTGGCGAATTGTAAAAGTAGCAACAACTAAGTCTCAAAGAA
AATTATTTTTTAATTTAAGAAAAAACAAAAAAAGATTAGGTTGGTTTAATCAAGAAGAAATTGAAATAGTTGCTCGAGAA
TTAGGTGTAAGCAGTGAAGATGTCAGAGAGATGGAGTCTAGAATGTCAGCTCAAGATATAACTTTTAATCCTTTTCCAGA
AGAAGATTTAAAAGATGGAAAAATTAATGGAAATATGTTTTATTTACAAGATAAAACATCTAATTTTGCAAATGGATTAG
AACAAGATAATTGGAATAAACATACTACAAGCAAACTAAGTAATGCTTTGTTAAGATTAGATGAGCGAAGTCGAAATATT
ATTCGTGCACGTTGGTTAGATAAGAAGGAAAAAAACACTTTGCAAAAAATTGCAAATAATTATGGAATATCTGCCGAGCG
TGTTAGACAATTAGAAAAAAATGCTATGAAAAAATTAAAAATAGCGATAGAAAATTAA

Upstream 100 bases:

>100_bases
TTTTTATAAATTTTTGATTTTAATTTTTAATTAAAGTATGATTAAATCATCCCATATATTTATAATTCATTAAATATATT
TTCTTAATACGGGAATTAAG

Downstream 100 bases:

>100_bases
TTTTATAATAGTATCAAGTTTTAAAAATTAATAATCTTAAAATTAAGATAATGATCTACATAGAGTATGAGATTAAGTTT
TTAAAATTGATCGCTCATAC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTNKVQILSVTALGNLDAYIRIANLWPMLSIKEEKLLTKRLRYHDDLDAAKTLILSHLRFVIHISRNYSGYGLLQADLIQ
EGNIGLMKAVRRFNPDIGVRLVSFAVHWIKSEIHEYVLRNWRIVKVATTKSQRKLFFNLRKNKKRLGWFNQEEIEIVARE
LGVSSEDVREMESRMSAQDITFNPFPEEDLKDGKINGNMFYLQDKTSNFANGLEQDNWNKHTTSKLSNALLRLDERSRNI
IRARWLDKKEKNTLQKIANNYGISAERVRQLEKNAMKKLKIAIEN

Sequences:

>Translated_285_residues
MTNKVQILSVTALGNLDAYIRIANLWPMLSIKEEKLLTKRLRYHDDLDAAKTLILSHLRFVIHISRNYSGYGLLQADLIQ
EGNIGLMKAVRRFNPDIGVRLVSFAVHWIKSEIHEYVLRNWRIVKVATTKSQRKLFFNLRKNKKRLGWFNQEEIEIVARE
LGVSSEDVREMESRMSAQDITFNPFPEEDLKDGKINGNMFYLQDKTSNFANGLEQDNWNKHTTSKLSNALLRLDERSRNI
IRARWLDKKEKNTLQKIANNYGISAERVRQLEKNAMKKLKIAIEN
>Mature_284_residues
TNKVQILSVTALGNLDAYIRIANLWPMLSIKEEKLLTKRLRYHDDLDAAKTLILSHLRFVIHISRNYSGYGLLQADLIQE
GNIGLMKAVRRFNPDIGVRLVSFAVHWIKSEIHEYVLRNWRIVKVATTKSQRKLFFNLRKNKKRLGWFNQEEIEIVAREL
GVSSEDVREMESRMSAQDITFNPFPEEDLKDGKINGNMFYLQDKTSNFANGLEQDNWNKHTTSKLSNALLRLDERSRNII
RARWLDKKEKNTLQKIANNYGISAERVRQLEKNAMKKLKIAIEN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily

Homologues:

Organism=Escherichia coli, GI1789871, Length=284, Percent_Identity=70.7746478873239, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1789098, Length=259, Percent_Identity=27.7992277992278, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=26.890756302521, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): RP32_BUCAP (Q8KA76)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660386.1
- ProteinModelPortal:   Q8KA76
- SMR:   Q8KA76
- EnsemblBacteria:   EBBUCT00000000465
- GeneID:   1005842
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg026
- GeneTree:   EBGT00050000007966
- HOGENOM:   HBG745096
- OMA:   DEDNEHA
- ProtClustDB:   PRK06596
- BioCyc:   BAPH198804:BUSG026-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02392
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 33299; Mature: 33168

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKVQILSVTALGNLDAYIRIANLWPMLSIKEEKLLTKRLRYHDDLDAAKTLILSHLRF
CCCCEEEEEEEEECCCHHEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VIHISRNYSGYGLLQADLIQEGNIGLMKAVRRFNPDIGVRLVSFAVHWIKSEIHEYVLRN
HEEEECCCCCCCEEEHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKSQRKLFFNLRKNKKRLGWFNQEEIEIVARELGVSSEDVREMESRMSAQDI
CEEEEEECCCHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
TFNPFPEEDLKDGKINGNMFYLQDKTSNFANGLEQDNWNKHTTSKLSNALLRLDERSRNI
CCCCCCCCCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IRARWLDKKEKNTLQKIANNYGISAERVRQLEKNAMKKLKIAIEN
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
TNKVQILSVTALGNLDAYIRIANLWPMLSIKEEKLLTKRLRYHDDLDAAKTLILSHLRF
CCCEEEEEEEEECCCHHEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VIHISRNYSGYGLLQADLIQEGNIGLMKAVRRFNPDIGVRLVSFAVHWIKSEIHEYVLRN
HEEEECCCCCCCEEEHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKSQRKLFFNLRKNKKRLGWFNQEEIEIVARELGVSSEDVREMESRMSAQDI
CEEEEEECCCHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
TFNPFPEEDLKDGKINGNMFYLQDKTSNFANGLEQDNWNKHTTSKLSNALLRLDERSRNI
CCCCCCCCCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IRARWLDKKEKNTLQKIANNYGISAERVRQLEKNAMKKLKIAIEN
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438