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The map label for this gene is dut [H]

Identifier: 30263974

GI number: 30263974

Start: 3779698

End: 3780144

Strand: Reverse

Name: dut [H]

Synonym: BA_4112

Alternate gene names: 30263974

Gene position: 3780144-3779698 (Counterclockwise)

Preceding gene: 30263975

Following gene: 30263973

Centisome position: 72.32

GC content: 45.41

Gene sequence:

>447_bases
ATGAAATTAAGAACTAAGATTAAGCGAGTAAGAGATGTGGAATTGCCTAAGTATGCCAAGACAGGGGATTCGGGTTTTGA
TCTTGTAGCAGCAGAAGACACGGTTATATGGCCAGGAGAAACAAAGGTTGTGCCGACTGGATTGGCATTTGAGATTCCAC
CAGGATATGAATTGCAGGTGAGGCCGCGTAGTGGTATGACGCGTAATACAAAGTTGAGAGTTGTTCTTGGAACGGTGGAT
AGTGGCTACCGTGGAGAAGTTGGGGTGCTGGTTGATAATACTGAAATCCCTAAAGCGACCAATATGCAAGCACATGTAAT
TGAAAAGGGTACACGCATCGCTCAAGGCGTTATAGTGCCAGTAATAACAGCACATTTTGAAGAAGTGGACGAGCTATCGG
ATTCAGAAAGGGGCTCAGGTGGTTTCGGGAGTACAGGGGTTAAGTAA

Upstream 100 bases:

>100_bases
AATCTTATCTAAATACAAGGAGGAAGAACAATGAAATATACAGAGCATGGTACTTACGAAGTAACTCAATTATTAGCAGA
AGCGAAGGAGACTGAAGAGA

Downstream 100 bases:

>100_bases
AACAAAATTTGAATTTTGTTAAGAAATGAGGGTGATTGAAATAAGTTGGTGGGCAATAGCGATCGGTTTATATCTATTGA
TTGGAGTTGCATTACTTATA

Product: prophage LambdaBa02, deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MKLRTKIKRVRDVELPKYAKTGDSGFDLVAAEDTVIWPGETKVVPTGLAFEIPPGYELQVRPRSGMTRNTKLRVVLGTVD
SGYRGEVGVLVDNTEIPKATNMQAHVIEKGTRIAQGVIVPVITAHFEEVDELSDSERGSGGFGSTGVK

Sequences:

>Translated_148_residues
MKLRTKIKRVRDVELPKYAKTGDSGFDLVAAEDTVIWPGETKVVPTGLAFEIPPGYELQVRPRSGMTRNTKLRVVLGTVD
SGYRGEVGVLVDNTEIPKATNMQAHVIEKGTRIAQGVIVPVITAHFEEVDELSDSERGSGGFGSTGVK
>Mature_148_residues
MKLRTKIKRVRDVELPKYAKTGDSGFDLVAAEDTVIWPGETKVVPTGLAFEIPPGYELQVRPRSGMTRNTKLRVVLGTVD
SGYRGEVGVLVDNTEIPKATNMQAHVIEKGTRIAQGVIVPVITAHFEEVDELSDSERGSGGFGSTGVK

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=123, Percent_Identity=40.650406504065, Blast_Score=85, Evalue=2e-17,
Organism=Homo sapiens, GI4503423, Length=123, Percent_Identity=40.650406504065, Blast_Score=85, Evalue=2e-17,
Organism=Homo sapiens, GI70906441, Length=123, Percent_Identity=40.650406504065, Blast_Score=84, Evalue=6e-17,
Organism=Escherichia coli, GI1790071, Length=141, Percent_Identity=36.8794326241135, Blast_Score=85, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988561, Length=137, Percent_Identity=40.1459854014599, Blast_Score=92, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6319729, Length=139, Percent_Identity=32.3741007194245, Blast_Score=71, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19921126, Length=125, Percent_Identity=37.6, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24583610, Length=125, Percent_Identity=37.6, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16038; Mature: 16038

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRTKIKRVRDVELPKYAKTGDSGFDLVAAEDTVIWPGETKVVPTGLAFEIPPGYELQV
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEECCCCCEEEECCEEEECCCCCEEEE
RPRSGMTRNTKLRVVLGTVDSGYRGEVGVLVDNTEIPKATNMQAHVIEKGTRIAQGVIVP
ECCCCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCHHHCCEEEE
VITAHFEEVDELSDSERGSGGFGSTGVK
HHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLRTKIKRVRDVELPKYAKTGDSGFDLVAAEDTVIWPGETKVVPTGLAFEIPPGYELQV
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEECCCCCEEEECCEEEECCCCCEEEE
RPRSGMTRNTKLRVVLGTVDSGYRGEVGVLVDNTEIPKATNMQAHVIEKGTRIAQGVIVP
ECCCCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCHHHCCEEEE
VITAHFEEVDELSDSERGSGGFGSTGVK
HHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA