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The map label for this gene is cwlA [H]

Identifier: 30263935

GI number: 30263935

Start: 3750163

End: 3750867

Strand: Reverse

Name: cwlA [H]

Synonym: BA_4073

Alternate gene names: 30263935

Gene position: 3750867-3750163 (Counterclockwise)

Preceding gene: 30263936

Following gene: 30263931

Centisome position: 71.76

GC content: 40.14

Gene sequence:

>705_bases
ATGGAAATCAGAAAAAAATTAGTTGTCCCAAGTAAATACGGTACAAAGTGTCCATATACAATGAAACCTAAATATATCAC
TGTTCATAACACATATAATGACGCTCCCGCTGAAAATGAAGTGAATTACATGATTACTAACAATAATGAAGTATCGTTTC
ATGTGGCAGTAGATGACAAGCAAGCAATTCAAGGTATTCCGTGGGAACGTAATGCGTGGGCTTGTGGTGACGGTAATGGT
CCAGGGAATCGGGAGTCCATTTCTGTAGAAATCTGTTATTCAAAATCAGGAGGAGATAGATACTATAAGGCTGAGAACAA
CGCTGTTGATGTTGTACGTCAACTCATGTCTATGTATAATATCCCGATTGAAAATGTTCGAACTCACCAATCATGGTCAG
GAAAATATTGCCCACATAGAATGCTAGCTGAAGGCAGATGGGGAGCGTTTATTCAGAAGGTTAAGAGCGGAAACGTAGCA
AGTGCAACGGTAACCCCTAAACAAAACATCATCCAAACAGGGGCGTTCTCACCGTACGAACTACCTGATGCGGTAGGTGC
TTTGAAATCACTGAACATGACAGGTAAGGCGATTATAAACCCGGAAGGATTGACTTATATCGTTACAGACCCGACGTCAG
ATGTACAGTTACAAGCTTTTAAAGAGTATCTCGAAAGAAAAGATTGGTGGTATGATGATAAATAA

Upstream 100 bases:

>100_bases
CCGGGCGAATGGGTATTCCACTCCCACAGGCTTTAACAAATGCAGTTGAAATTTTAGGTGGTAAACAAAAACAAGAAGAG
AAAAAAGGAGATGTTGAATA

Downstream 100 bases:

>100_bases
AACAAAAGAATAGTTTTGTAAAAAAGGGTCTACTCATACTTGAGCAGACCCTTTTTTATTTCATTATGGAATGGATACAA
TCACGCTATTATTATACATT

Product: prophage LambdaBa02, N-acetylmuramoyl-L-alanine amidase family protein 2

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MEIRKKLVVPSKYGTKCPYTMKPKYITVHNTYNDAPAENEVNYMITNNNEVSFHVAVDDKQAIQGIPWERNAWACGDGNG
PGNRESISVEICYSKSGGDRYYKAENNAVDVVRQLMSMYNIPIENVRTHQSWSGKYCPHRMLAEGRWGAFIQKVKSGNVA
SATVTPKQNIIQTGAFSPYELPDAVGALKSLNMTGKAIINPEGLTYIVTDPTSDVQLQAFKEYLERKDWWYDDK

Sequences:

>Translated_234_residues
MEIRKKLVVPSKYGTKCPYTMKPKYITVHNTYNDAPAENEVNYMITNNNEVSFHVAVDDKQAIQGIPWERNAWACGDGNG
PGNRESISVEICYSKSGGDRYYKAENNAVDVVRQLMSMYNIPIENVRTHQSWSGKYCPHRMLAEGRWGAFIQKVKSGNVA
SATVTPKQNIIQTGAFSPYELPDAVGALKSLNMTGKAIINPEGLTYIVTDPTSDVQLQAFKEYLERKDWWYDDK
>Mature_234_residues
MEIRKKLVVPSKYGTKCPYTMKPKYITVHNTYNDAPAENEVNYMITNNNEVSFHVAVDDKQAIQGIPWERNAWACGDGNG
PGNRESISVEICYSKSGGDRYYKAENNAVDVVRQLMSMYNIPIENVRTHQSWSGKYCPHRMLAEGRWGAFIQKVKSGNVA
SATVTPKQNIIQTGAFSPYELPDAVGALKSLNMTGKAIINPEGLTYIVTDPTSDVQLQAFKEYLERKDWWYDDK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26414; Mature: 26414

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRKKLVVPSKYGTKCPYTMKPKYITVHNTYNDAPAENEVNYMITNNNEVSFHVAVDDK
CCCCCCEECCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCEEEEECCCEEEEEEEECCH
QAIQGIPWERNAWACGDGNGPGNRESISVEICYSKSGGDRYYKAENNAVDVVRQLMSMYN
HHHCCCCCCCCEEECCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHC
IPIENVRTHQSWSGKYCPHRMLAEGRWGAFIQKVKSGNVASATVTPKQNIIQTGAFSPYE
CCHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCEEEEEECCHHHHHHCCCCCCCC
LPDAVGALKSLNMTGKAIINPEGLTYIVTDPTSDVQLQAFKEYLERKDWWYDDK
CHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEIRKKLVVPSKYGTKCPYTMKPKYITVHNTYNDAPAENEVNYMITNNNEVSFHVAVDDK
CCCCCCEECCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCEEEEECCCEEEEEEEECCH
QAIQGIPWERNAWACGDGNGPGNRESISVEICYSKSGGDRYYKAENNAVDVVRQLMSMYN
HHHCCCCCCCCEEECCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHC
IPIENVRTHQSWSGKYCPHRMLAEGRWGAFIQKVKSGNVASATVTPKQNIIQTGAFSPYE
CCHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCEEEEEECCHHHHHHCCCCCCCC
LPDAVGALKSLNMTGKAIINPEGLTYIVTDPTSDVQLQAFKEYLERKDWWYDDK
CHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2127796; 1683402; 7704261; 8969508; 9384377 [H]