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The map label for this gene is pbpB [H]

Identifier: 30263918

GI number: 30263918

Start: 3736653

End: 3738803

Strand: Reverse

Name: pbpB [H]

Synonym: BA_4055

Alternate gene names: 30263918

Gene position: 3738803-3736653 (Counterclockwise)

Preceding gene: 30263919

Following gene: 30263917

Centisome position: 71.52

GC content: 38.03

Gene sequence:

>2151_bases
ATGAAGAGAAATATGACTAAATTTCATACCAATAAGGGAGCAGGGTATTTTATGATTTTATTCCTCCTGCTCTTTTTGCT
GTTATTAGCCCGATTTTTTTACATACAAGCAACAGGATCAGTTCATAATCAAGATTTGAATGAACTTGCTAAGCAAAAAC
ATAGTAAAACAGGAGTTCTAGAAGCAAATCGCGGGACGATCTATGATCAAAATGGTCATGTATTAGCGCAAGATGCAAAC
TCTTATAAACTTGTAGCGGAATTAAAAGGTGCGAAGCCGGTTGAGGATAAGGAGGATACCGCCAAGAAAATTGCGGGCGT
ACTCGGAAAAGATGAAGAGAAGATTTTAGCTACGTTAAATAAAGAAGATAGAAGTCAAGTGGAATTTGGGAAATTAGGTA
AGGGCTTGACGAAAGAACAAAAGGAACAAATTGAAGCATTAAAATTGCCAGGTATATCTTTTATTACTGAAAATGCAAGA
GTATATCCGAACGGTGATTTCGCATCTTATGTCGTAGGTCATGCGAGACCTGATGATAAGGGGATTGCTATAGGGCAATT
TGGTTTAGAAAAGAGTTTGGATAAGTATTTAGGAGCTTCTCATGGGGAAGTAGCTTATACAGGCGATCGTAGAGGCGTAT
CTCTTGACGGTGGAAAAGTAAACGTAAAAGCACCTAAAAATGGAGATAATGTATATTTAACGCTTGACCAACGTATTCAA
AGTTATTTAGAAGATGCAATGAAAGAAGCGAGTAAACATTATGAACCAGAAAGCTTAATAGGAATTGTTGCGGATCCAAA
AACAGGGAAAATATTAGGAATGTCTAGTAAGCCTAGTTATGATCCGAACCAGGAAAATAATCAGTATTTTTATAATGATG
CAATCGCAAATGCATTTGAACCTGGATCAACAATGAAAATTTTCACACTAGCAGCAGCTATTAATGAAGGTGTGTATAAG
GGACAAGATTATTATCAGTCTGGTACATATCCAGTTGGGAACCGCAAAATAAAAGATCATAATGGCGGCGCTGGATGGGG
ATCTATCACATTTGATGAAGGAGTAGAGCGCTCTTCAAACGTAGCATTTGCAATTTTAGGGGATCAAAAACTTGGTCCAG
AACGTTTTCGAAAATATATTCATAGCTTTGGATTAGATGAAAAAACAAAGATTGATTTACCTGGTGAAGGTTCAAATACC
ATTGTATTCGATCAGCAAATACAGCAAGTAACAACAGCTTTTGGACAAGGTTCTACTGTAACACCAATTCAGCTTGTCCA
AGCTGCAACTGCTATTGCGAACGATGGGAAAATGATGAAACCTTATACAATTGATAAAATTGTAGATCCGATAACAGAGG
AAGTAAAATTAGAACATAAACCAGAAGAAGTGGGAAAACCTGTTACAAAAGAGACAGCAGCGCAAGTAAGACAGTTATTA
GAGCGTGTTGTTACATCACCGAAAGGAACAGGAACTGCTTATAAAGTCGATGGATATTCAGTTGGTGGTAAAACAGGAAC
AGCACAAATTCCGGATGGAAAAGGTGGCTATATGACGGGAAGAGAGAATTATATATTCTCGTTCTTAGGTATGGCACCTA
TGGACGATCCGCAACTTGTTGTGTATGTAGCTGTTAAAAAGCCGAAATTGAAAGATGATGAGAGCGGTGCACAACCTTTA
GCTGATATTTTTAAATATGTAACGAAGAATAGTTTAGAGTATTTAAAGATTAAGCCTAATGAAGTGAAAGATCCGAAGAA
ATATGTGAAAGAGCAACAAACCGCTGTTCCAGATGTAACCGAAAAAACGATGGAAGAAGCGAAAAAAGCCATTGATAAAG
CAAAACTACGTCCAATCGTATTAGGTGAAGGGAAAGTACAGCAACAAGTGCCGAAAGCAACTGAACAAACATTGAAGGGT
GACCGAGTCTTCTTAGTGGGAGATAAACCTACAATGCCGAATATACAAGGCTGGGCACTGCGTGATGTTATGAATTTAGC
AAAAACATTAAAGCTTAACCTAAAACCTACTGGTACCGGATACGTTACTGAACAAAGTGTGCCAGAAGGAACATTGTTGC
AACCTGGTACAGAGTTAGGTGTAACACTTGTACCGCCACTTGAACCACAACAAGAAGCAGAAAAACCGTAA

Upstream 100 bases:

>100_bases
TCGTTATGAACGAATCGCTGAAGTTGCAAAAAAACACGGGCTAGAAATTAATGCAAATAATGTGAAAGGCCTCAAATAAG
GCAATAGGTGTGAAGGGAAA

Downstream 100 bases:

>100_bases
TGGTAAAAGAGAAGATGTTCTAATTAAATAAGTACAAGCATATATTGGAACAAGCTAGGCGTAAGGGAGGCTTGTTCCAA
TATGCGTGTATCAAATGTAA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MKRNMTKFHTNKGAGYFMILFLLLFLLLLARFFYIQATGSVHNQDLNELAKQKHSKTGVLEANRGTIYDQNGHVLAQDAN
SYKLVAELKGAKPVEDKEDTAKKIAGVLGKDEEKILATLNKEDRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENAR
VYPNGDFASYVVGHARPDDKGIAIGQFGLEKSLDKYLGASHGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQ
SYLEDAMKEASKHYEPESLIGIVADPKTGKILGMSSKPSYDPNQENNQYFYNDAIANAFEPGSTMKIFTLAAAINEGVYK
GQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPERFRKYIHSFGLDEKTKIDLPGEGSNT
IVFDQQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLL
ERVVTSPKGTGTAYKVDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYVAVKKPKLKDDESGAQPL
ADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVTEKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKG
DRVFLVGDKPTMPNIQGWALRDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP

Sequences:

>Translated_716_residues
MKRNMTKFHTNKGAGYFMILFLLLFLLLLARFFYIQATGSVHNQDLNELAKQKHSKTGVLEANRGTIYDQNGHVLAQDAN
SYKLVAELKGAKPVEDKEDTAKKIAGVLGKDEEKILATLNKEDRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENAR
VYPNGDFASYVVGHARPDDKGIAIGQFGLEKSLDKYLGASHGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQ
SYLEDAMKEASKHYEPESLIGIVADPKTGKILGMSSKPSYDPNQENNQYFYNDAIANAFEPGSTMKIFTLAAAINEGVYK
GQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPERFRKYIHSFGLDEKTKIDLPGEGSNT
IVFDQQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLL
ERVVTSPKGTGTAYKVDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYVAVKKPKLKDDESGAQPL
ADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVTEKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKG
DRVFLVGDKPTMPNIQGWALRDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP
>Mature_716_residues
MKRNMTKFHTNKGAGYFMILFLLLFLLLLARFFYIQATGSVHNQDLNELAKQKHSKTGVLEANRGTIYDQNGHVLAQDAN
SYKLVAELKGAKPVEDKEDTAKKIAGVLGKDEEKILATLNKEDRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENAR
VYPNGDFASYVVGHARPDDKGIAIGQFGLEKSLDKYLGASHGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQ
SYLEDAMKEASKHYEPESLIGIVADPKTGKILGMSSKPSYDPNQENNQYFYNDAIANAFEPGSTMKIFTLAAAINEGVYK
GQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPERFRKYIHSFGLDEKTKIDLPGEGSNT
IVFDQQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLL
ERVVTSPKGTGTAYKVDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYVAVKKPKLKDDESGAQPL
ADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVTEKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKG
DRVFLVGDKPTMPNIQGWALRDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=605, Percent_Identity=27.603305785124, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1786854, Length=551, Percent_Identity=23.2304900181488, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 78583; Mature: 78583

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNMTKFHTNKGAGYFMILFLLLFLLLLARFFYIQATGSVHNQDLNELAKQKHSKTGVL
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCCE
EANRGTIYDQNGHVLAQDANSYKLVAELKGAKPVEDKEDTAKKIAGVLGKDEEKILATLN
ECCCCEEEECCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCC
KEDRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENARVYPNGDFASYVVGHARPDDK
CCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHEEECCCCCCC
GIAIGQFGLEKSLDKYLGASHGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQ
CEEEECCCHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEECCCCCCEEEEEEHHHHH
SYLEDAMKEASKHYEPESLIGIVADPKTGKILGMSSKPSYDPNQENNQYFYNDAIANAFE
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCEEECHHHHHCCC
PGSTMKIFTLAAAINEGVYKGQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSN
CCCCEEHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCC
VAFAILGDQKLGPERFRKYIHSFGLDEKTKIDLPGEGSNTIVFDQQIQQVTTAFGQGSTV
EEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHCCCCCC
TPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLL
CHHHHHHHHHHHHCCCCEECCEEHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHH
ERVVTSPKGTGTAYKVDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLV
HHHHCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEE
VYVAVKKPKLKDDESGAQPLADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVT
EEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHH
EKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKGDRVFLVGDKPTMPNIQGWAL
HHHHHHHHHHHHHHHCCEEEECCCHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHH
RDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP
HHHHHHHHHEEEECCCCCCCEEECCCCCCCCEECCCCCCCEEEECCCCCHHHCCCC
>Mature Secondary Structure
MKRNMTKFHTNKGAGYFMILFLLLFLLLLARFFYIQATGSVHNQDLNELAKQKHSKTGVL
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCCE
EANRGTIYDQNGHVLAQDANSYKLVAELKGAKPVEDKEDTAKKIAGVLGKDEEKILATLN
ECCCCEEEECCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCC
KEDRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENARVYPNGDFASYVVGHARPDDK
CCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHEEECCCCCCC
GIAIGQFGLEKSLDKYLGASHGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQ
CEEEECCCHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEECCCCCCEEEEEEHHHHH
SYLEDAMKEASKHYEPESLIGIVADPKTGKILGMSSKPSYDPNQENNQYFYNDAIANAFE
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCEEECHHHHHCCC
PGSTMKIFTLAAAINEGVYKGQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSN
CCCCEEHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCC
VAFAILGDQKLGPERFRKYIHSFGLDEKTKIDLPGEGSNTIVFDQQIQQVTTAFGQGSTV
EEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHCCCCCC
TPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLL
CHHHHHHHHHHHHCCCCEECCEEHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHH
ERVVTSPKGTGTAYKVDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLV
HHHHCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEE
VYVAVKKPKLKDDESGAQPLADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVT
EEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHH
EKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKGDRVFLVGDKPTMPNIQGWAL
HHHHHHHHHHHHHHHCCEEEECCCHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHH
RDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP
HHHHHHHHHEEEECCCCCCCEEECCCCCCCCEECCCCCCCEEEECCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]