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The map label for this gene is pyrF

Identifier: 30263886

GI number: 30263886

Start: 3700096

End: 3700812

Strand: Reverse

Name: pyrF

Synonym: BA_4022

Alternate gene names: 30263886

Gene position: 3700812-3700096 (Counterclockwise)

Preceding gene: 30263887

Following gene: 30263885

Centisome position: 70.8

GC content: 40.17

Gene sequence:

>717_bases
ATGTCACAGTCGTTAATCGTTGCACTAGATTTCCCAGGGAAACAAGATGTAGAACAATTCTTGCGCCACTTTGAAGGGGA
AGAGTTATTTGTCAAAGTTGGTATGGAGTTATTTTACAAAGAAGGCCCTGCGATTATTACGTACTTAAAAGAAAAAGGAC
ATAAAATCTTTCTAGATTTAAAACTTCATGATATTCCGAATACAGTAAAAAGCGCTATGCGTAGCCTAGCTAGTCTAGAT
GTAGATATGGTAAATGTTCATGCTGGTGGGGGAAGCAGTATGATGAAAGCTGCGATTGAGGGATTAGAGGAAGGTAAGCA
AGAAGGAAAAGAGAGACCGATTTGTATTGCAGTTACACAACTAACAAGCACTTCGGAAACTATGATGAAAAAAGAGATTG
GCATTGAGAAAACGTTAGAAGAAGCGGTTGCTCATTATGCAAAACTAACGAAAGAAAGTGGACTTGATGGCGTTGTTTGC
TCAACACTTGAAGTTCCAAAATTACGTGAAGTATGCGGAAGTGAATTTGTAACAGTAACACCGGGGATTCGTCTTGCAAG
CGATGATGTAAATGACCAGGTGCGCGTAGCAACACCGAAACGTGCGAGGGAACTTGGCTCAAGCTATATCGTAGTTGGAC
GTAGTATTACAAAAGCAGAAAATCCGCTTGAAGCGTATAAAACAGTAAAACAACAGTGGGAAGGTGTAACAGTATGA

Upstream 100 bases:

>100_bases
CGTTCGTATGTCCGACAATTATTGAGGAGTTACCAGCATTACTAGATGAATTAGGATTTGATCACATTTCGGAATGTCAA
GGAAGGAGCTGGAAGCAAAC

Downstream 100 bases:

>100_bases
AAAAAGAAATCGCATCGCATTTATTAGAAATTGGAGCAGTATTTTTACAACCAAATGATCCATTCACATGGTCTTCAGGT
ATGAAATCACCAATTTATTG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSQSLIVALDFPGKQDVEQFLRHFEGEELFVKVGMELFYKEGPAIITYLKEKGHKIFLDLKLHDIPNTVKSAMRSLASLD
VDMVNVHAGGGSSMMKAAIEGLEEGKQEGKERPICIAVTQLTSTSETMMKKEIGIEKTLEEAVAHYAKLTKESGLDGVVC
STLEVPKLREVCGSEFVTVTPGIRLASDDVNDQVRVATPKRARELGSSYIVVGRSITKAENPLEAYKTVKQQWEGVTV

Sequences:

>Translated_238_residues
MSQSLIVALDFPGKQDVEQFLRHFEGEELFVKVGMELFYKEGPAIITYLKEKGHKIFLDLKLHDIPNTVKSAMRSLASLD
VDMVNVHAGGGSSMMKAAIEGLEEGKQEGKERPICIAVTQLTSTSETMMKKEIGIEKTLEEAVAHYAKLTKESGLDGVVC
STLEVPKLREVCGSEFVTVTPGIRLASDDVNDQVRVATPKRARELGSSYIVVGRSITKAENPLEAYKTVKQQWEGVTV
>Mature_237_residues
SQSLIVALDFPGKQDVEQFLRHFEGEELFVKVGMELFYKEGPAIITYLKEKGHKIFLDLKLHDIPNTVKSAMRSLASLDV
DMVNVHAGGGSSMMKAAIEGLEEGKQEGKERPICIAVTQLTSTSETMMKKEIGIEKTLEEAVAHYAKLTKESGLDGVVCS
TLEVPKLREVCGSEFVTVTPGIRLASDDVNDQVRVATPKRARELGSSYIVVGRSITKAENPLEAYKTVKQQWEGVTV

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=226, Percent_Identity=40.7079646017699, Blast_Score=167, Evalue=8e-43,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_BACAA (C3P653)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868119.1
- ProteinModelPortal:   C3P653
- SMR:   C3P653
- EnsemblBacteria:   EBBACT00000126763
- GeneID:   7852551
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4045
- GeneTree:   EBGT00050000000955
- ProtClustDB:   PRK00230
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 26188; Mature: 26056

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 61-61 BINDING 10-10 BINDING 32-32 BINDING 122-122 BINDING 184-184 BINDING 193-193 BINDING 213-213 BINDING 214-214

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSLIVALDFPGKQDVEQFLRHFEGEELFVKVGMELFYKEGPAIITYLKEKGHKIFLDL
CCCCEEEEEECCCHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCEEEEEEHHCCCEEEEEE
KLHDIPNTVKSAMRSLASLDVDMVNVHAGGGSSMMKAAIEGLEEGKQEGKERPICIAVTQ
EECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
LTSTSETMMKKEIGIEKTLEEAVAHYAKLTKESGLDGVVCSTLEVPKLREVCGSEFVTVT
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHCCCEEEEC
PGIRLASDDVNDQVRVATPKRARELGSSYIVVGRSITKAENPLEAYKTVKQQWEGVTV
CCCEEECCCCCCCEEEECCHHHHHCCCCEEEECCCHHCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SQSLIVALDFPGKQDVEQFLRHFEGEELFVKVGMELFYKEGPAIITYLKEKGHKIFLDL
CCCEEEEEECCCHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCEEEEEEHHCCCEEEEEE
KLHDIPNTVKSAMRSLASLDVDMVNVHAGGGSSMMKAAIEGLEEGKQEGKERPICIAVTQ
EECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
LTSTSETMMKKEIGIEKTLEEAVAHYAKLTKESGLDGVVCSTLEVPKLREVCGSEFVTVT
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHCCCEEEEC
PGIRLASDDVNDQVRVATPKRARELGSSYIVVGRSITKAENPLEAYKTVKQQWEGVTV
CCCEEECCCCCCCEEEECCHHHHHCCCCEEEECCCHHCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA