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The map label for this gene is pyrE

Identifier: 30263885

GI number: 30263885

Start: 3699467

End: 3700099

Strand: Reverse

Name: pyrE

Synonym: BA_4021

Alternate gene names: 30263885

Gene position: 3700099-3699467 (Counterclockwise)

Preceding gene: 30263886

Following gene: 30263882

Centisome position: 70.78

GC content: 38.86

Gene sequence:

>633_bases
ATGAAAAAAGAAATCGCATCGCATTTATTAGAAATTGGAGCAGTATTTTTACAACCAAATGATCCATTCACATGGTCTTC
AGGTATGAAATCACCAATTTATTGTGATAACCGTTTAACTTTATCTTATCCGAAAGTACGCCAAACAATTGCAGCTGGAT
TAGAAGAGTTAATTAAAGAGCACTTCCCAACTGTAGAAGTTATTGCAGGAACAGCAACTGCTGGTATTGCGCACGCTGCA
TGGGTAAGCGATCGTATGGATTTACCAATGTGCTACGTACGTAGTAAAGCAAAAGGTCATGGTAAAGGGAACCAAATCGA
AGGAAAAGCTGAAAAAGGTCAAAAAGTAGTAGTAGTAGAAGACTTAATTTCAACTGGTGGTAGTGCTATTACATGTGTAG
AAGCACTTCGCGAAGCTGGCTGTGAAGTATTAGGAATCGTATCAATCTTCACATACGAGCTAGAAGCAGGAAAAGAAAAG
CTAGAAGCAGCTAACGTAGCATCATATTCTTTAAGTGATTACAGTGCATTAACTGAAGTTGCGGCAGAAAAAGGTATAAT
CGGACAAGCTGAAACGAAAAAATTACAAGAGTGGCGTAAAAATCCAGCTGACGAAGCTTGGATCACAGCGTAA

Upstream 100 bases:

>100_bases
TTGGCTCAAGCTATATCGTAGTTGGACGTAGTATTACAAAAGCAGAAAATCCGCTTGAAGCGTATAAAACAGTAAAACAA
CAGTGGGAAGGTGTAACAGT

Downstream 100 bases:

>100_bases
CACGAAAAAAAGACGACTGCGGGGGAACGCGGTCGTCTTTTTTATATCATTGTTAAAACTTCATGCAAAGAGTGTATAGT
GGTTACATCGTGTGTTAGCT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKKEIASHLLEIGAVFLQPNDPFTWSSGMKSPIYCDNRLTLSYPKVRQTIAAGLEELIKEHFPTVEVIAGTATAGIAHAA
WVSDRMDLPMCYVRSKAKGHGKGNQIEGKAEKGQKVVVVEDLISTGGSAITCVEALREAGCEVLGIVSIFTYELEAGKEK
LEAANVASYSLSDYSALTEVAAEKGIIGQAETKKLQEWRKNPADEAWITA

Sequences:

>Translated_210_residues
MKKEIASHLLEIGAVFLQPNDPFTWSSGMKSPIYCDNRLTLSYPKVRQTIAAGLEELIKEHFPTVEVIAGTATAGIAHAA
WVSDRMDLPMCYVRSKAKGHGKGNQIEGKAEKGQKVVVVEDLISTGGSAITCVEALREAGCEVLGIVSIFTYELEAGKEK
LEAANVASYSLSDYSALTEVAAEKGIIGQAETKKLQEWRKNPADEAWITA
>Mature_210_residues
MKKEIASHLLEIGAVFLQPNDPFTWSSGMKSPIYCDNRLTLSYPKVRQTIAAGLEELIKEHFPTVEVIAGTATAGIAHAA
WVSDRMDLPMCYVRSKAKGHGKGNQIEGKAEKGQKVVVVEDLISTGGSAITCVEALREAGCEVLGIVSIFTYELEAGKEK
LEAANVASYSLSDYSALTEVAAEKGIIGQAETKKLQEWRKNPADEAWITA

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=162, Percent_Identity=25.3086419753086, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508631, Length=179, Percent_Identity=29.608938547486, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=170, Percent_Identity=25.8823529411765, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17933654, Length=176, Percent_Identity=27.8409090909091, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_BACAA (C3P652)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868118.1
- ProteinModelPortal:   C3P652
- SMR:   C3P652
- EnsemblBacteria:   EBBACT00000130391
- GeneID:   7848484
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4044
- GeneTree:   EBGT00050000002725
- ProtClustDB:   PRK00455
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 22714; Mature: 22714

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 94-94 BINDING 98-98 BINDING 100-100 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEIASHLLEIGAVFLQPNDPFTWSSGMKSPIYCDNRLTLSYPKVRQTIAAGLEELIKE
CHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCEEECCEEEECCHHHHHHHHHHHHHHHHH
HFPTVEVIAGTATAGIAHAAWVSDRMDLPMCYVRSKAKGHGKGNQIEGKAEKGQKVVVVE
HCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCHHCCCCEEEEEH
DLISTGGSAITCVEALREAGCEVLGIVSIFTYELEAGKEKLEAANVASYSLSDYSALTEV
HHHHCCCCHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AAEKGIIGQAETKKLQEWRKNPADEAWITA
HHHCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKKEIASHLLEIGAVFLQPNDPFTWSSGMKSPIYCDNRLTLSYPKVRQTIAAGLEELIKE
CHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCEEECCEEEECCHHHHHHHHHHHHHHHHH
HFPTVEVIAGTATAGIAHAAWVSDRMDLPMCYVRSKAKGHGKGNQIEGKAEKGQKVVVVE
HCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCHHCCCCEEEEEH
DLISTGGSAITCVEALREAGCEVLGIVSIFTYELEAGKEKLEAANVASYSLSDYSALTEV
HHHHCCCCHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AAEKGIIGQAETKKLQEWRKNPADEAWITA
HHHCCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA