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The map label for this gene is sigW [H]

Identifier: 30263538

GI number: 30263538

Start: 3357821

End: 3358330

Strand: Reverse

Name: sigW [H]

Synonym: BA_3649

Alternate gene names: 30263538

Gene position: 3358330-3357821 (Counterclockwise)

Preceding gene: 30263539

Following gene: 30263537

Centisome position: 64.25

GC content: 31.96

Gene sequence:

>510_bases
ATGATTGTAAAAGAAGAAACGGATGTTTCCTTAGCGAAAAAAGGTGATCATGAAGCATTTATGACGCTTATACATACAGA
AAAAGTAAAGATGTATCGTATTGCAAAGGCTATGCTACGTGATGAGACAAATATAGAGGATGCGATACAAACAACAATTT
TAAAAGCCTATGAAAATATAAAAAAATTAAAGAAGGAAGAATTTTTTCAAACTTGGTTAATTCGAATTTTAATGAATGAA
TGTAATAACATTATTAGAGCGTATAAAAATGTAATTGTGACAGAAGAGAATGATTACAATATGAGTGCGTGCGATCAGTA
TGAAGATATAGATTTGTGTAATGCGATTCAATCGTTACATGAAGAGTTAAGAGCTGTTACGGTGCTTTATTATTATGAAG
ATATGAATCAAGACAGTATAGCGAAGCTTTTAGAAGTACCAAAAGGAACAGTGAAATCGAGATTGTCACGTGCGAGGGAA
CAACTACAGAAACGATTAAAGATGGAATAG

Upstream 100 bases:

>100_bases
GAACCTTTTCCTAGGTTCCTACGTCTAATACATGTAAAGTTAAATTTGAAGTGAGGATGAAACAGGAGGAGCAATTGTGA
AAGTAATCCCTTTATATAAA

Downstream 100 bases:

>100_bases
GGGGTGCAAAGGATGGATGATGAAAAATTATTTGATGAGAAATTAAAAAAGAGAATAAACGAAGAGAATGTAATAGTGCC
GCCAGAGTTAAATGAAAAGA

Product: ECF subfamily RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MIVKEETDVSLAKKGDHEAFMTLIHTEKVKMYRIAKAMLRDETNIEDAIQTTILKAYENIKKLKKEEFFQTWLIRILMNE
CNNIIRAYKNVIVTEENDYNMSACDQYEDIDLCNAIQSLHEELRAVTVLYYYEDMNQDSIAKLLEVPKGTVKSRLSRARE
QLQKRLKME

Sequences:

>Translated_169_residues
MIVKEETDVSLAKKGDHEAFMTLIHTEKVKMYRIAKAMLRDETNIEDAIQTTILKAYENIKKLKKEEFFQTWLIRILMNE
CNNIIRAYKNVIVTEENDYNMSACDQYEDIDLCNAIQSLHEELRAVTVLYYYEDMNQDSIAKLLEVPKGTVKSRLSRARE
QLQKRLKME
>Mature_169_residues
MIVKEETDVSLAKKGDHEAFMTLIHTEKVKMYRIAKAMLRDETNIEDAIQTTILKAYENIKKLKKEEFFQTWLIRILMNE
CNNIIRAYKNVIVTEENDYNMSACDQYEDIDLCNAIQSLHEELRAVTVLYYYEDMNQDSIAKLLEVPKGTVKSRLSRARE
QLQKRLKME

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=173, Percent_Identity=24.8554913294798, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 19946; Mature: 19946

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVKEETDVSLAKKGDHEAFMTLIHTEKVKMYRIAKAMLRDETNIEDAIQTTILKAYENI
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KKLKKEEFFQTWLIRILMNECNNIIRAYKNVIVTEENDYNMSACDQYEDIDLCNAIQSLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHCCCCCHHHHHHHHHHH
EELRAVTVLYYYEDMNQDSIAKLLEVPKGTVKSRLSRAREQLQKRLKME
HHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIVKEETDVSLAKKGDHEAFMTLIHTEKVKMYRIAKAMLRDETNIEDAIQTTILKAYENI
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KKLKKEEFFQTWLIRILMNECNNIIRAYKNVIVTEENDYNMSACDQYEDIDLCNAIQSLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHCCCCCHHHHHHHHHHH
EELRAVTVLYYYEDMNQDSIAKLLEVPKGTVKSRLSRAREQLQKRLKME
HHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]