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The map label for this gene is cls [H]

Identifier: 21245022

GI number: 21245022

Start: 5096421

End: 5097644

Strand: Direct

Name: cls [H]

Synonym: XAC4310

Alternate gene names: 21245022

Gene position: 5096421-5097644 (Clockwise)

Preceding gene: 21245021

Following gene: 21245024

Centisome position: 98.47

GC content: 65.2

Gene sequence:

>1224_bases
GTGGGGCTGCTGCTGCTCAACTTCGCCACGCCGGAGAAAAAGCTCGAGCACATCCCCAAACACCTCTACGACGTTGCCGA
TCCGCAATTCAAACGCGAGATGTCGGTGTTGCTGGGGCCGGCCATCCTGCCCGGCAACAGGATCGACGTGCTCAATAACG
GCCGCGAGATCTTTCCGGCCATGCTGGAGGCCATCCGCAGCGCGCAGCACACCATCACGTTCGAAACCTATATCTATTGG
TCGGGCGAGATCGGCCGAGAATTCAGCGACGCCTTGTCCGAGCGCGCGCGCGCCGGGGTGGATGTGCGGGTCACCATCGA
CTGGGGCGGCAGCCTGAAGATGGACCATGCACTGGTAGACACCATGACCGAGGCCGGCGTGGACGTGCACCGCTACCGCC
CGCTGGCCTGGTACAACCTGCACCGCATCAACAACCGCACGCACCGCAAGCTGCTGGTGATCGACGGCCGTATCGGCTTC
ACCGGCGGCGTGGGCGTGGCCGACCAGTGGATGGGCGACGCGCAGGATCCGGACCATTGGCGCGACACCCACTACCGCAT
CGAAGGCCCGGTGGTGGCGCAGGTGCAGACCGCCTTCAACGACAACTGGATCAAGACCACCGGCCGCGTGGTCAATGGCG
CCCGGTACTACCCGGCGCTGGAGGCGGTCGGCGACAGCGATGCGCAGTTGTTCGTGGCCTCGCCGGCCGGCGGCAGCGAA
AGCATGCACCTGATGTATCTGATCGCCATCGCCGCCGCATGCAGCACCATCGACCTGGCCGCGGCGTATTTCGTGCCCGA
TGCGCTGATCACCCGCGCGCTGCTGGAAGCGCGCAGTCGCGGCGTCAGCATCCGCGTGCTGCTGCCCGGCAAGCACATCG
ATGCGGTGTCGGTGCGGCTGGCCTCCAAGGCCAGCTGGGAGCCACTGCTGCAGGCCGGCATCGAGATCCACGAATACCAG
CCCACCATGCTGCATACCAAACTGCTGATCATCGACGGCCTGTTGGTCTCGGTAGGCTCCACCAATTTCGACATCCGCTC
GTTCCGCCTCAACGACGAGGCCAGCCTGAATGTCTACGACCGCACGCTGGCTGCACGCATGACCGAGGTGTTCGAGCGCG
ATCTGCAACGCGCCGAGCAGTATTCGCTGGAGCGCTGGCGCGCGCGGCCGTTGCGGCAGAAGCTTGGCGAGAAACTGGTG
TTCCCGTTCCGCTCGCAGGTGTGA

Upstream 100 bases:

>100_bases
CTTGCCAGAATGCCGGCCGCTTGGGGCTGTCGATGGCAGGGGAACCGGAGTTGGATGCATGACATGGATCATCGTGGCCG
CCGTGGCGGTCACCTTGCTG

Downstream 100 bases:

>100_bases
GCGCGCAGGGCCGATAACGGCGGTGTTAGCGTGCCGCCCCCGCCGCGCCTGCCTGCAAGCGCAGCTGACGCAGGCGCCAC
CACAGCCCGCCCACGTGCAC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 1 [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MGLLLLNFATPEKKLEHIPKHLYDVADPQFKREMSVLLGPAILPGNRIDVLNNGREIFPAMLEAIRSAQHTITFETYIYW
SGEIGREFSDALSERARAGVDVRVTIDWGGSLKMDHALVDTMTEAGVDVHRYRPLAWYNLHRINNRTHRKLLVIDGRIGF
TGGVGVADQWMGDAQDPDHWRDTHYRIEGPVVAQVQTAFNDNWIKTTGRVVNGARYYPALEAVGDSDAQLFVASPAGGSE
SMHLMYLIAIAAACSTIDLAAAYFVPDALITRALLEARSRGVSIRVLLPGKHIDAVSVRLASKASWEPLLQAGIEIHEYQ
PTMLHTKLLIIDGLLVSVGSTNFDIRSFRLNDEASLNVYDRTLAARMTEVFERDLQRAEQYSLERWRARPLRQKLGEKLV
FPFRSQV

Sequences:

>Translated_407_residues
MGLLLLNFATPEKKLEHIPKHLYDVADPQFKREMSVLLGPAILPGNRIDVLNNGREIFPAMLEAIRSAQHTITFETYIYW
SGEIGREFSDALSERARAGVDVRVTIDWGGSLKMDHALVDTMTEAGVDVHRYRPLAWYNLHRINNRTHRKLLVIDGRIGF
TGGVGVADQWMGDAQDPDHWRDTHYRIEGPVVAQVQTAFNDNWIKTTGRVVNGARYYPALEAVGDSDAQLFVASPAGGSE
SMHLMYLIAIAAACSTIDLAAAYFVPDALITRALLEARSRGVSIRVLLPGKHIDAVSVRLASKASWEPLLQAGIEIHEYQ
PTMLHTKLLIIDGLLVSVGSTNFDIRSFRLNDEASLNVYDRTLAARMTEVFERDLQRAEQYSLERWRARPLRQKLGEKLV
FPFRSQV
>Mature_406_residues
GLLLLNFATPEKKLEHIPKHLYDVADPQFKREMSVLLGPAILPGNRIDVLNNGREIFPAMLEAIRSAQHTITFETYIYWS
GEIGREFSDALSERARAGVDVRVTIDWGGSLKMDHALVDTMTEAGVDVHRYRPLAWYNLHRINNRTHRKLLVIDGRIGFT
GGVGVADQWMGDAQDPDHWRDTHYRIEGPVVAQVQTAFNDNWIKTTGRVVNGARYYPALEAVGDSDAQLFVASPAGGSES
MHLMYLIAIAAACSTIDLAAAYFVPDALITRALLEARSRGVSIRVLLPGKHIDAVSVRLASKASWEPLLQAGIEIHEYQP
TMLHTKLLIIDGLLVSVGSTNFDIRSFRLNDEASLNVYDRTLAARMTEVFERDLQRAEQYSLERWRARPLRQKLGEKLVF
PFRSQV

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=401, Percent_Identity=28.1795511221945, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787007, Length=350, Percent_Identity=30.2857142857143, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI87081826, Length=409, Percent_Identity=24.6943765281174, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 45730; Mature: 45599

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLLLNFATPEKKLEHIPKHLYDVADPQFKREMSVLLGPAILPGNRIDVLNNGREIFPA
CCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEECCCCEEEEECCCHHHHHH
MLEAIRSAQHTITFETYIYWSGEIGREFSDALSERARAGVDVRVTIDWGGSLKMDHALVD
HHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEHHHHHH
TMTEAGVDVHRYRPLAWYNLHRINNRTHRKLLVIDGRIGFTGGVGVADQWMGDAQDPDHW
HHHHCCCCHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCC
RDTHYRIEGPVVAQVQTAFNDNWIKTTGRVVNGARYYPALEAVGDSDAQLFVASPAGGSE
CCCCEEECCCEEEEEEHHCCCCEEEECCCEECCCCCCCHHHHCCCCCCEEEEECCCCCCC
SMHLMYLIAIAAACSTIDLAAAYFVPDALITRALLEARSRGVSIRVLLPGKHIDAVSVRL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEE
ASKASWEPLLQAGIEIHEYQPTMLHTKLLIIDGLLVSVGSTNFDIRSFRLNDEASLNVYD
CCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCHHH
RTLAARMTEVFERDLQRAEQYSLERWRARPLRQKLGEKLVFPFRSQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHEECCHHCCC
>Mature Secondary Structure 
GLLLLNFATPEKKLEHIPKHLYDVADPQFKREMSVLLGPAILPGNRIDVLNNGREIFPA
CEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEECCCCEEEEECCCHHHHHH
MLEAIRSAQHTITFETYIYWSGEIGREFSDALSERARAGVDVRVTIDWGGSLKMDHALVD
HHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEHHHHHH
TMTEAGVDVHRYRPLAWYNLHRINNRTHRKLLVIDGRIGFTGGVGVADQWMGDAQDPDHW
HHHHCCCCHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCC
RDTHYRIEGPVVAQVQTAFNDNWIKTTGRVVNGARYYPALEAVGDSDAQLFVASPAGGSE
CCCCEEECCCEEEEEEHHCCCCEEEECCCEECCCCCCCHHHHCCCCCCEEEEECCCCCCC
SMHLMYLIAIAAACSTIDLAAAYFVPDALITRALLEARSRGVSIRVLLPGKHIDAVSVRL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEE
ASKASWEPLLQAGIEIHEYQPTMLHTKLLIIDGLLVSVGSTNFDIRSFRLNDEASLNVYD
CCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCHHH
RTLAARMTEVFERDLQRAEQYSLERWRARPLRQKLGEKLVFPFRSQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377 [H]