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The map label for this gene is leuC [H]

Identifier: 21244183

GI number: 21244183

Start: 4080186

End: 4081625

Strand: Reverse

Name: leuC [H]

Synonym: XAC3458

Alternate gene names: 21244183

Gene position: 4081625-4080186 (Counterclockwise)

Preceding gene: 77748715

Following gene: 21244182

Centisome position: 78.86

GC content: 65.62

Gene sequence:

>1440_bases
ATGACTGCCAGGACCCTGTACGACAAGCTGTGGGAACTGCACGAGGTCACACGCCGTGACGATGGCTCGTCGCTGATCTA
CATCGACCGCCACATCCTGCACGAAGTGACCTCGCCGCAGGCCTTCGAAGGCCTGCGCCTGGCCGGGCGCAAGCCGTGGC
GCATCGACGCCAATATCGCCACGCCCGATCACAACGTGCCGACCACGCGCGCCGAACGCCAGGGCGGGCTGGAGTCGATT
TCCGACGAGGTCTCGCGCCTGCAGGTGCAGACGCTGGACGAAAACTGCGACGACTTCGGCATCCTCGAGTTCAAGATGAA
CGATGCGCGGCAGGGCATCGTGCATGTGGTCGGCCCGGAGCAGGGCGCCACCTTGCCGGGCATGACCGTGGTCTGCGGCG
ATTCGCATACCTCCACGCATGGCGCGTTCGGCGCGCTGGCGCACGGCATCGGCACCTCCGAGGTCGAGCACGTGCTGGCC
ACCCAATGCCTGATCGCCAAGAAAATGAAGAACATGCAGGTGCGTGTGGAAGGCACGCTGCCGTTCGGGGTGACCGCCAA
GGACATCGTGCTGGCGGTGATCGGCAAGATCGGCACCGCCGGCGGCAACGGGCATGCGCTGGAATTTGCCGGCAGCGCCA
TTCGGACTCTGTCGATGGAAGGCCGCATGACCATCTGCAACATGGCCATCGAAGCCGGTGCCCGCGTGGGCATGGTGGCG
GTGGACGAGAAGACCATTGCCTACGTCAGAGGGCGCCCGTTCGCCCCCAAGGGCGCCCACTGGGATGCGGCGGTGGCGCT
CTGGAGCACGCTGGTCTCCGACCCGGACGCGCACTTCGACACCGTGGTGGAGTTGCGTGCCGAAGACATCAAGCCGCAGG
TCAGCTGGGGCACTTCGCCGGAAATGGTGCTGGCCATCGATCAGCACGTGCCCGATCCGGCCATCGAGCAGGACCCGACC
AAGCGCGATTCGATCGAGCGCGCGCTGAAATACATGGGGCTGAAGGCCAACCAGCCGATCACCGCCATCCGCCTGGATCG
CGTGTTCATCGGCTCGTGCACCAATTCGCGCATCGAAGACCTGCGTGCCGCTGCCGCAGTGGCCAAGGGCCGCAAGGTCG
CCTCCACCATCAAGCAGGCCTTGGTGGTGCCGGGCTCGGGGCTGGTGAAGGCGCAGGCCGAAGCCGAAGGCCTGGACAAG
GTGTTTCTGGATGCCGGCTTCGAATGGCGCGAGCCAGGCTGCTCGATGTGTCTGGCCATGAACCCGGACAAGCTGGGCAG
CGGCGAACATTGCGCTTCGACCTCCAACCGCAACTTCGAAGGCCGCCAAGGCGCCGGCGGCCGCACGCATCTGGTCAGCC
CGGCGATGGCGGCGGCTGCGGCGGTGAGCGGTCATTTTGTCGATGTACGTGAATTGCAGGGAATCGAGAGTCGGGAATAG

Upstream 100 bases:

>100_bases
TCTTGAATAAACAATATGAATTTGCGTAATCCAAGGCAAGCTCCTAGCATCGATCTCAGCCAGGTGGGCGCACCTACCAT
CCGCCACTCGGATTGCCGCG

Downstream 100 bases:

>100_bases
AGAATGGCAACGTCCGTCCCGTCTCCGCCCTGCCAATTCCCCATTCTCCACTCCCAATTCCCGGCATTCCAATGACTCCC
TTCACCCGACACACCGGATT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTARTLYDKLWELHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRKPWRIDANIATPDHNVPTTRAERQGGLESI
SDEVSRLQVQTLDENCDDFGILEFKMNDARQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLA
TQCLIAKKMKNMQVRVEGTLPFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRTLSMEGRMTICNMAIEAGARVGMVA
VDEKTIAYVRGRPFAPKGAHWDAAVALWSTLVSDPDAHFDTVVELRAEDIKPQVSWGTSPEMVLAIDQHVPDPAIEQDPT
KRDSIERALKYMGLKANQPITAIRLDRVFIGSCTNSRIEDLRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDK
VFLDAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELQGIESRE

Sequences:

>Translated_479_residues
MTARTLYDKLWELHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRKPWRIDANIATPDHNVPTTRAERQGGLESI
SDEVSRLQVQTLDENCDDFGILEFKMNDARQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLA
TQCLIAKKMKNMQVRVEGTLPFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRTLSMEGRMTICNMAIEAGARVGMVA
VDEKTIAYVRGRPFAPKGAHWDAAVALWSTLVSDPDAHFDTVVELRAEDIKPQVSWGTSPEMVLAIDQHVPDPAIEQDPT
KRDSIERALKYMGLKANQPITAIRLDRVFIGSCTNSRIEDLRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDK
VFLDAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELQGIESRE
>Mature_478_residues
TARTLYDKLWELHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRKPWRIDANIATPDHNVPTTRAERQGGLESIS
DEVSRLQVQTLDENCDDFGILEFKMNDARQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
QCLIAKKMKNMQVRVEGTLPFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRTLSMEGRMTICNMAIEAGARVGMVAV
DEKTIAYVRGRPFAPKGAHWDAAVALWSTLVSDPDAHFDTVVELRAEDIKPQVSWGTSPEMVLAIDQHVPDPAIEQDPTK
RDSIERALKYMGLKANQPITAIRLDRVFIGSCTNSRIEDLRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDKV
FLDAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELQGIESRE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=27.8236914600551, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI41352693, Length=382, Percent_Identity=26.7015706806283, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1786259, Length=471, Percent_Identity=63.4819532908705, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI1787531, Length=366, Percent_Identity=26.775956284153, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081781, Length=357, Percent_Identity=23.249299719888, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=365, Percent_Identity=28.2191780821918, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=367, Percent_Identity=28.6103542234332, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=27.3615635179153, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=457, Percent_Identity=27.3522975929978, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=63.0573248407643, Blast_Score=605, Evalue=1e-174,
Organism=Saccharomyces cerevisiae, GI6323335, Length=362, Percent_Identity=28.1767955801105, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=362, Percent_Identity=28.1767955801105, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320440, Length=357, Percent_Identity=27.7310924369748, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28571643, Length=363, Percent_Identity=26.9972451790634, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI281365315, Length=388, Percent_Identity=26.5463917525773, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17864292, Length=388, Percent_Identity=26.5463917525773, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161076999, Length=388, Percent_Identity=26.5463917525773, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137564, Length=382, Percent_Identity=26.9633507853403, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24645686, Length=381, Percent_Identity=26.7716535433071, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51507; Mature: 51376

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARTLYDKLWELHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRKPWRIDANIA
CCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCCEEEECEEC
TPDHNVPTTRAERQGGLESISDEVSRLQVQTLDENCDDFGILEFKMNDARQGIVHVVGPE
CCCCCCCCCHHHHCCCHHHHHHHHHHEEHHHHHCCCCCCEEEEEEECCCCCCEEEEECCC
QGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLIAKKMKNMQVRVEGTL
CCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
PFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRTLSMEGRMTICNMAIEAGARVGMVA
CCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCEEEEHHHHHCCCCEEEEE
VDEKTIAYVRGRPFAPKGAHWDAAVALWSTLVSDPDAHFDTVVELRAEDIKPQVSWGTSP
ECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
EMVLAIDQHVPDPAIEQDPTKRDSIERALKYMGLKANQPITAIRLDRVFIGSCTNSRIED
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECEEEEECCCCHHHHH
LRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDKVFLDAGFEWREPGCSMCLAM
HHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECHHHCCCHHHHHHCCCCCCCCCCCEEEEE
NPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELQGIESRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEHHHHCCCCCCC
>Mature Secondary Structure 
TARTLYDKLWELHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRKPWRIDANIA
CHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCCEEEECEEC
TPDHNVPTTRAERQGGLESISDEVSRLQVQTLDENCDDFGILEFKMNDARQGIVHVVGPE
CCCCCCCCCHHHHCCCHHHHHHHHHHEEHHHHHCCCCCCEEEEEEECCCCCCEEEEECCC
QGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLIAKKMKNMQVRVEGTL
CCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
PFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRTLSMEGRMTICNMAIEAGARVGMVA
CCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCEEEEHHHHHCCCCEEEEE
VDEKTIAYVRGRPFAPKGAHWDAAVALWSTLVSDPDAHFDTVVELRAEDIKPQVSWGTSP
ECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
EMVLAIDQHVPDPAIEQDPTKRDSIERALKYMGLKANQPITAIRLDRVFIGSCTNSRIED
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECEEEEECCCCHHHHH
LRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDKVFLDAGFEWREPGCSMCLAM
HHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECHHHCCCHHHHHHCCCCCCCCCCCEEEEE
NPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELQGIESRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA