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The map label for this gene is yfiF [H]

Identifier: 21244041

GI number: 21244041

Start: 3901881

End: 3902798

Strand: Reverse

Name: yfiF [H]

Synonym: XAC3316

Alternate gene names: 21244041

Gene position: 3902798-3901881 (Counterclockwise)

Preceding gene: 21244042

Following gene: 21244040

Centisome position: 75.41

GC content: 69.61

Gene sequence:

>918_bases
ATGACACAGCATGATGGACGCGGCGGACGGCCGCCACGCGATGGCGGCTCCGGCCCCTCGCACAATCCGTGGGGCCGCGC
TGCGCCGCGCACACCGGCGCCGCGCAGCGCCGCACCCCGTGCTCCAGCGCAGCACGCTCCAGCCGCCACGCCATCTGCCG
CTGCAGCGAGCGGCAACGCGCGTGAGGTGCGGCTGTACGGCATCAATGCCGTCCAGGCCGTGTTCAAGACGCGCCCGGAG
GCGCTGCGCAAGCTGTACCTGAGCGAGGCGCGCATTCCGCAATTCAAGGCGCTGCTGGCCTGGTGCGTGACCCAGCGCAT
CGGTTATCGCGTGGTGGAAGAGGCCGATCTGAGCAAGCTCGCTGCCAGTGCGCACCATGAGGGAGTGGTGGCCGAGGTGC
TGCGCGTCACCCCGCACCCCTTGCAGGAATGGCTGGCCGCGTTGCCGCAGGGCCCGGTGCTGGCGCTATGGCTGGACGGG
GTGGGCAACCCGCATAACTTCGGGGCGATCTTGCGCTCGTCGGCGCACTTCGGCGTGGCCGGTCTGTTGCTGCCGGCCGG
CTCCACGCTGGGCCTGTCGGGCGCGGCCGCACGCGTGGCCGAAGGCGGCGCCGAAGCGGTACCGCTGGTGCAATTGCCCG
ACACCGCAACGGCGTTGGCGCAGCTGCGTCAGGCCGGTTTTGCGATTGCTGCCACACTGGTCGACGGTGGCCAGGATGTC
TTCGCCGCCGCGTTGCCGCAACGCCTGGTATACGTGATGGGCGCAGAAAGCGACGGTATGGACCGCGCATTTGCGCGCGA
TTGCGATCTGCAGTTGTCGATCCGCGGCAGCGGCAAGGTGGAAAGTCTCAACGTTGCCTCGGCCACCGCGGTCTTCCAGG
CCGCCTGGCACGCGCGCATGCTCACCGGAGAACACTGA

Upstream 100 bases:

>100_bases
GCGGCCCTGCGGGTGCTGCAACACGATCGACACGCATACGGCGAAACGGCCGGAAACAGCCGTTTGGCACTGCAAGCAAC
ACGAGCTTCTGGATCCGACA

Downstream 100 bases:

>100_bases
TGTTGCGTTTTTCTGCGTTGTTGCTTGCGACCGGTTGCTTCTTCAGTGCAAACGCGTCTGCCACCACGACGGCGGCACCA
CCCGAGGTGCGTACCGATCA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTQHDGRGGRPPRDGGSGPSHNPWGRAAPRTPAPRSAAPRAPAQHAPAATPSAAAASGNAREVRLYGINAVQAVFKTRPE
ALRKLYLSEARIPQFKALLAWCVTQRIGYRVVEEADLSKLAASAHHEGVVAEVLRVTPHPLQEWLAALPQGPVLALWLDG
VGNPHNFGAILRSSAHFGVAGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATALAQLRQAGFAIAATLVDGGQDV
FAAALPQRLVYVMGAESDGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHARMLTGEH

Sequences:

>Translated_305_residues
MTQHDGRGGRPPRDGGSGPSHNPWGRAAPRTPAPRSAAPRAPAQHAPAATPSAAAASGNAREVRLYGINAVQAVFKTRPE
ALRKLYLSEARIPQFKALLAWCVTQRIGYRVVEEADLSKLAASAHHEGVVAEVLRVTPHPLQEWLAALPQGPVLALWLDG
VGNPHNFGAILRSSAHFGVAGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATALAQLRQAGFAIAATLVDGGQDV
FAAALPQRLVYVMGAESDGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHARMLTGEH
>Mature_304_residues
TQHDGRGGRPPRDGGSGPSHNPWGRAAPRTPAPRSAAPRAPAQHAPAATPSAAAASGNAREVRLYGINAVQAVFKTRPEA
LRKLYLSEARIPQFKALLAWCVTQRIGYRVVEEADLSKLAASAHHEGVVAEVLRVTPHPLQEWLAALPQGPVLALWLDGV
GNPHNFGAILRSSAHFGVAGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATALAQLRQAGFAIAATLVDGGQDVF
AAALPQRLVYVMGAESDGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHARMLTGEH

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=308, Percent_Identity=35.0649350649351, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1790623, Length=244, Percent_Identity=34.0163934426229, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31782; Mature: 31651

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHDGRGGRPPRDGGSGPSHNPWGRAAPRTPAPRSAAPRAPAQHAPAATPSAAAASGNA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
REVRLYGINAVQAVFKTRPEALRKLYLSEARIPQFKALLAWCVTQRIGYRVVEEADLSKL
CEEEEECHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
AASAHHEGVVAEVLRVTPHPLQEWLAALPQGPVLALWLDGVGNPHNFGAILRSSAHFGVA
HHHHHCCHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCHH
GLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATALAQLRQAGFAIAATLVDGGQDV
HHEECCCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCHHH
FAAALPQRLVYVMGAESDGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHARM
HHHHHHHHEEEEECCCCCCCCHHHHCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHH
LTGEH
HCCCC
>Mature Secondary Structure 
TQHDGRGGRPPRDGGSGPSHNPWGRAAPRTPAPRSAAPRAPAQHAPAATPSAAAASGNA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
REVRLYGINAVQAVFKTRPEALRKLYLSEARIPQFKALLAWCVTQRIGYRVVEEADLSKL
CEEEEECHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
AASAHHEGVVAEVLRVTPHPLQEWLAALPQGPVLALWLDGVGNPHNFGAILRSSAHFGVA
HHHHHCCHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCHH
GLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATALAQLRQAGFAIAATLVDGGQDV
HHEECCCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCHHH
FAAALPQRLVYVMGAESDGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHARM
HHHHHHHHEEEEECCCCCCCCHHHHCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHH
LTGEH
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]