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The map label for this gene is nth [H]

Identifier: 21242332

GI number: 21242332

Start: 1829361

End: 1830026

Strand: Reverse

Name: nth [H]

Synonym: XAC1582

Alternate gene names: 21242332

Gene position: 1830026-1829361 (Counterclockwise)

Preceding gene: 21242333

Following gene: 21242329

Centisome position: 35.36

GC content: 61.71

Gene sequence:

>666_bases
ATGCGCAAGCCCGAGATACAGGAAATGTTCGAGCGCCTGCGTGAACTCAATCCGCACCCCACCACCGAATTGGAGTACAC
CACCCCATTCGAGTTATTGATCGCGGTGCTGCTGTCGGCGCAAGCCACCGATGTCGGCGTCAACAAGGCGACCCGCAAGC
TATATCCGGTGGCCAATACCCCGCGCGACATTCTCGATCTCGGCGAAGAAGGCCTGAAGCGGTACATCTCCACCATCGGC
TTGTTCAACGCCAAGGCCAAGAACGTCATCGCCACCTGCCGCATCCTGCTCGAGCGCTATGGCGGCGACGTGCCGCATGA
TCGCGCCGCGCTGGAAGCGCTGCCCGGCGTTGGCCGCAAGACCGCAAACGTGGTGCTCAACACCGCCTTCGGCGAGCCCG
CCATGGCCGTGGATACGCATATCTTTCGCGTCTCCAACCGCACCGGTCTTGCGCCAGGCAAGGACGTACGCGCGGTCGAA
GACAAGTTGGTCAAGGTGATTCCGTCCGAATTCCTGCACGATGCGCATCACTGGCTGATCCTGCACGGACGCTACGTCTG
CAAGGCACGCAAACCGGATTGCCCGGGCTGCGTGATCCACGATCTATGCCGCTACCGCGACAAGACGCCGCCGGCCCCTG
AGCGGCAAACAAAAACCAGGAGTTGA

Upstream 100 bases:

>100_bases
TGGCCGCTGCCATCCTGGCGCGCGCAAAGGCGAAAGCCGCTGGCGGAGCGCAGCCATGAACATACCTCAGGCAACGGCGC
CGACGCGTCGTGGCGGCAGC

Downstream 100 bases:

>100_bases
TGCAGCAATGTAGCCGGAGCAACGACGCAACCGCGCGCCCTGATCCACGCGCAGCAACCGCAAAGCCGCAAGCTTTTACG
AATCCCGAATCCCCACTCGC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MRKPEIQEMFERLRELNPHPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLYPVANTPRDILDLGEEGLKRYISTIG
LFNAKAKNVIATCRILLERYGGDVPHDRAALEALPGVGRKTANVVLNTAFGEPAMAVDTHIFRVSNRTGLAPGKDVRAVE
DKLVKVIPSEFLHDAHHWLILHGRYVCKARKPDCPGCVIHDLCRYRDKTPPAPERQTKTRS

Sequences:

>Translated_221_residues
MRKPEIQEMFERLRELNPHPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLYPVANTPRDILDLGEEGLKRYISTIG
LFNAKAKNVIATCRILLERYGGDVPHDRAALEALPGVGRKTANVVLNTAFGEPAMAVDTHIFRVSNRTGLAPGKDVRAVE
DKLVKVIPSEFLHDAHHWLILHGRYVCKARKPDCPGCVIHDLCRYRDKTPPAPERQTKTRS
>Mature_221_residues
MRKPEIQEMFERLRELNPHPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLYPVANTPRDILDLGEEGLKRYISTIG
LFNAKAKNVIATCRILLERYGGDVPHDRAALEALPGVGRKTANVVLNTAFGEPAMAVDTHIFRVSNRTGLAPGKDVRAVE
DKLVKVIPSEFLHDAHHWLILHGRYVCKARKPDCPGCVIHDLCRYRDKTPPAPERQTKTRS

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=178, Percent_Identity=32.0224719101124, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=71.6346153846154, Blast_Score=315, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=32.2033898305085, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=192, Percent_Identity=24.4791666666667, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=30.5555555555556, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24753; Mature: 24753

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPEIQEMFERLRELNPHPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLYPVANT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCC
PRDILDLGEEGLKRYISTIGLFNAKAKNVIATCRILLERYGGDVPHDRAALEALPGVGRK
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCH
TANVVLNTAFGEPAMAVDTHIFRVSNRTGLAPGKDVRAVEDKLVKVIPSEFLHDAHHWLI
HHHEEEECCCCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
LHGRYVCKARKPDCPGCVIHDLCRYRDKTPPAPERQTKTRS
EECEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKPEIQEMFERLRELNPHPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLYPVANT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCC
PRDILDLGEEGLKRYISTIGLFNAKAKNVIATCRILLERYGGDVPHDRAALEALPGVGRK
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCH
TANVVLNTAFGEPAMAVDTHIFRVSNRTGLAPGKDVRAVEDKLVKVIPSEFLHDAHHWLI
HHHEEEECCCCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
LHGRYVCKARKPDCPGCVIHDLCRYRDKTPPAPERQTKTRS
EECEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]