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The map label for this gene is phoX [H]

Identifier: 21242328

GI number: 21242328

Start: 1823671

End: 1824690

Strand: Reverse

Name: phoX [H]

Synonym: XAC1578

Alternate gene names: 21242328

Gene position: 1824690-1823671 (Counterclockwise)

Preceding gene: 21242329

Following gene: 21242327

Centisome position: 35.26

GC content: 63.92

Gene sequence:

>1020_bases
GTGATCCACTCCTTCAAGACCCGTCTGGCCGTCGGCGTGCTCGCCGCTTCGCTGGCACTGTGCGCCCAGGCTGCCGACGT
CACCGGTGCCGGTGCGTCGTTCATCTACCCGGTGATGTCCAAGTGGTCGGCCGACTACAACGCGGCCACCAAGAAGCAGG
TCAACTACCAATCGATCGGTTCGGGCGGCGGCATTGCCCAGATCAAGGCGGCCAGCGTGGACTTCGGCTCTTCCGATGCC
CCGCTCAAGCCGGAAGAACTGGCCGCTGCCGGCCTGGCGCAGTTTCCCTCGGTGATCGGCGGCGTGGTACCGGTGGTCAA
CGTGCCGGGCATTGCCGCCGGTACGCTCAAGTTGGACGGCAAGACTCTGGGCGACATCTTCCTGGGCAAGGTCAGCACCT
GGAACGACCCGGCCATCGCCGCGCTCAACCCGGGCGTGAAACTGCCGGAAGGCAAGATCACCGTGGTGCACCGCTCCGAC
GGCTCGGGCACCAGCTTCAACTTCACCAACTACCTCTCCAAGGTCAACCCGGACTGGAAGGGCAAGGTCGGCGAAGGCAC
GGCGGTGCAGTGGCCGACCGGCATCGGCGGCAAGGGCAACGAGGGCGTGGCTGCCTACGTGAAGCAGATCAAGGGCGGCA
TCGGCTATGTCGAGCTGTCGTACGCGCTGCAGAACAAGATGGCCTACACCGCAATGAAGAACGCGGCCGGCAAGTTCGTG
CAGCCGTCGGATGAAACCTTCGCCGCCGCTGCCAACAGCGCCGACTGGGGCACGGCCAAGGACTTCTATCTGGTGATGAC
CAACGCGGCCGGCGATAACGCCTGGCCGATCACGGCGACCAACTTCATCCTGGTGCAGAAAAAGCCGAAGAATCCGGCTG
GACTGAAGAACACGCTGGAATTCTTCCGCTGGGTCTACACCAAGGGCGATGCGCAGGCCAAACAGTTGGACTACGTGCCG
CTGCCGGACACGCTGGTGACCCAGATCGAGGCGTACTGGGCCAAGAATCTGCCGCACTAA

Upstream 100 bases:

>100_bases
TCATCGGCCTGTCATGTCCCAGACACCGCATGGTCATGCCGTTGCCCTGCAATGGCACCACGGCGGGACGTCCTCATCCC
TTCGCTACAGGAGCTGTTCC

Downstream 100 bases:

>100_bases
GAGTACGGCGCAGGCCTCTGCTGCAGCGGCCTGGTGGGGCAAGCCATGCTCTAAAACGGCATGGGCCGTTGTCTGGTCCA
TGCCGGCGAGCCGCCCGCCC

Product: phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MIHSFKTRLAVGVLAASLALCAQAADVTGAGASFIYPVMSKWSADYNAATKKQVNYQSIGSGGGIAQIKAASVDFGSSDA
PLKPEELAAAGLAQFPSVIGGVVPVVNVPGIAAGTLKLDGKTLGDIFLGKVSTWNDPAIAALNPGVKLPEGKITVVHRSD
GSGTSFNFTNYLSKVNPDWKGKVGEGTAVQWPTGIGGKGNEGVAAYVKQIKGGIGYVELSYALQNKMAYTAMKNAAGKFV
QPSDETFAAAANSADWGTAKDFYLVMTNAAGDNAWPITATNFILVQKKPKNPAGLKNTLEFFRWVYTKGDAQAKQLDYVP
LPDTLVTQIEAYWAKNLPH

Sequences:

>Translated_339_residues
MIHSFKTRLAVGVLAASLALCAQAADVTGAGASFIYPVMSKWSADYNAATKKQVNYQSIGSGGGIAQIKAASVDFGSSDA
PLKPEELAAAGLAQFPSVIGGVVPVVNVPGIAAGTLKLDGKTLGDIFLGKVSTWNDPAIAALNPGVKLPEGKITVVHRSD
GSGTSFNFTNYLSKVNPDWKGKVGEGTAVQWPTGIGGKGNEGVAAYVKQIKGGIGYVELSYALQNKMAYTAMKNAAGKFV
QPSDETFAAAANSADWGTAKDFYLVMTNAAGDNAWPITATNFILVQKKPKNPAGLKNTLEFFRWVYTKGDAQAKQLDYVP
LPDTLVTQIEAYWAKNLPH
>Mature_339_residues
MIHSFKTRLAVGVLAASLALCAQAADVTGAGASFIYPVMSKWSADYNAATKKQVNYQSIGSGGGIAQIKAASVDFGSSDA
PLKPEELAAAGLAQFPSVIGGVVPVVNVPGIAAGTLKLDGKTLGDIFLGKVSTWNDPAIAALNPGVKLPEGKITVVHRSD
GSGTSFNFTNYLSKVNPDWKGKVGEGTAVQWPTGIGGKGNEGVAAYVKQIKGGIGYVELSYALQNKMAYTAMKNAAGKFV
QPSDETFAAAANSADWGTAKDFYLVMTNAAGDNAWPITATNFILVQKKPKNPAGLKNTLEFFRWVYTKGDAQAKQLDYVP
LPDTLVTQIEAYWAKNLPH

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=313, Percent_Identity=56.2300319488818, Blast_Score=357, Evalue=1e-100,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 35624; Mature: 35624

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHSFKTRLAVGVLAASLALCAQAADVTGAGASFIYPVMSKWSADYNAATKKQVNYQSIG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCHHHCC
SGGGIAQIKAASVDFGSSDAPLKPEELAAAGLAQFPSVIGGVVPVVNVPGIAAGTLKLDG
CCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHEECCCCCEEEEEEECC
KTLGDIFLGKVSTWNDPAIAALNPGVKLPEGKITVVHRSDGSGTSFNFTNYLSKVNPDWK
CEECHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCEEHHHHHHHCCCCCC
GKVGEGTAVQWPTGIGGKGNEGVAAYVKQIKGGIGYVELSYALQNKMAYTAMKNAAGKFV
CCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCC
QPSDETFAAAANSADWGTAKDFYLVMTNAAGDNAWPITATNFILVQKKPKNPAGLKNTLE
CCCCCHHEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEEECCCCCCCHHHHHH
FFRWVYTKGDAQAKQLDYVPLPDTLVTQIEAYWAKNLPH
HHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIHSFKTRLAVGVLAASLALCAQAADVTGAGASFIYPVMSKWSADYNAATKKQVNYQSIG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCHHHCC
SGGGIAQIKAASVDFGSSDAPLKPEELAAAGLAQFPSVIGGVVPVVNVPGIAAGTLKLDG
CCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHEECCCCCEEEEEEECC
KTLGDIFLGKVSTWNDPAIAALNPGVKLPEGKITVVHRSDGSGTSFNFTNYLSKVNPDWK
CEECHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCEEHHHHHHHCCCCCC
GKVGEGTAVQWPTGIGGKGNEGVAAYVKQIKGGIGYVELSYALQNKMAYTAMKNAAGKFV
CCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCC
QPSDETFAAAANSADWGTAKDFYLVMTNAAGDNAWPITATNFILVQKKPKNPAGLKNTLE
CCCCCHHEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEEECCCCCCCHHHHHH
FFRWVYTKGDAQAKQLDYVPLPDTLVTQIEAYWAKNLPH
HHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]