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The map label for this gene is rnhB

Identifier: 21242160

GI number: 21242160

Start: 1621794

End: 1622528

Strand: Reverse

Name: rnhB

Synonym: XAC1407

Alternate gene names: 21242160

Gene position: 1622528-1621794 (Counterclockwise)

Preceding gene: 21242161

Following gene: 21242159

Centisome position: 31.35

GC content: 67.76

Gene sequence:

>735_bases
ATGAGGCGCTCTACCTCCAATGGCGCGGTTGTCGTTCCGGCGACGCAGGACGGGCTTTTCCACGATTCCCCATTCCCCAT
TCCCGATTCCCGCCTGATCGCCGGTGTCGACGAAGCTGGCCGCGGCCCGCTGGCCGGGCCGGTGGCCGTTGCCGCCGTGG
TGTTCGACCCCGGCAAGCCGCGCATCAACGGGCTGGACGATTCCAAGCAGCTCACGGCCGAGCGGCGCGAGCAGCTGTAT
GCGCGCATCGTCGACCGGGCGCTGGCGTGGTCGGTGGTGTTGATCGACAGCGAAGAGATCGACCGCATCAATATCTACCA
GGCGACCATGCTCGGCATGCGGCGCGCAGTGGAAGGCGTGGCGCATGTGGCCGGGTTCGCGCGCATCGACGGCAACCGCG
TGCCCAAGGGGCTGCCCTGCCCGGCCGAGGCATTGATCGGTGGCGACGCGTTGGACCGCGCCATCATGGCCGCCTCGATC
GTGGCCAAGGTGACGCGCGACCGGCTGATGCACGAACTGCACACGCGCCACCCGGAATATCGCTTCGATCAGCACAAGGG
CTACAGCACGCCGGTGCATCTGGCCGCGTTGCAAACCCACGGGCCATGCCCGCAACATCGGCGCAGTTTTGCGCCGGTGC
GTCTGGCGCTGCAAGGCCGTGAAGGCCTGGAACCGGATCCCGGGACCCGGGACCTGGAGCAGTTGCAGGTCGGGCAACTG
GTTGCGCCGCTGTAA

Upstream 100 bases:

>100_bases
TGCGCCGCGATGCGTCGGCGCGGGCAGCGGATGCGGTGGCTGGGCTGCTGTCGCAGCGCGAATCGGGAATGGAGAATCGG
GAATCGGCAGGAGCCGGCGC

Downstream 100 bases:

>100_bases
GAGACGCCAGGGTCGGCATCGGCGTGTCCGGCTGCGCGTCGGAGCCTGAACAGCCGCACATCGCGTGCCGTGCGCAGTAG
CTCCAGGCACACATCATTCC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRRSTSNGAVVVPATQDGLFHDSPFPIPDSRLIAGVDEAGRGPLAGPVAVAAVVFDPGKPRINGLDDSKQLTAERREQLY
ARIVDRALAWSVVLIDSEEIDRINIYQATMLGMRRAVEGVAHVAGFARIDGNRVPKGLPCPAEALIGGDALDRAIMAASI
VAKVTRDRLMHELHTRHPEYRFDQHKGYSTPVHLAALQTHGPCPQHRRSFAPVRLALQGREGLEPDPGTRDLEQLQVGQL
VAPL

Sequences:

>Translated_244_residues
MRRSTSNGAVVVPATQDGLFHDSPFPIPDSRLIAGVDEAGRGPLAGPVAVAAVVFDPGKPRINGLDDSKQLTAERREQLY
ARIVDRALAWSVVLIDSEEIDRINIYQATMLGMRRAVEGVAHVAGFARIDGNRVPKGLPCPAEALIGGDALDRAIMAASI
VAKVTRDRLMHELHTRHPEYRFDQHKGYSTPVHLAALQTHGPCPQHRRSFAPVRLALQGREGLEPDPGTRDLEQLQVGQL
VAPL
>Mature_244_residues
MRRSTSNGAVVVPATQDGLFHDSPFPIPDSRLIAGVDEAGRGPLAGPVAVAAVVFDPGKPRINGLDDSKQLTAERREQLY
ARIVDRALAWSVVLIDSEEIDRINIYQATMLGMRRAVEGVAHVAGFARIDGNRVPKGLPCPAEALIGGDALDRAIMAASI
VAKVTRDRLMHELHTRHPEYRFDQHKGYSTPVHLAALQTHGPCPQHRRSFAPVRLALQGREGLEPDPGTRDLEQLQVGQL
VAPL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=193, Percent_Identity=52.3316062176166, Blast_Score=186, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_XANAC (Q8PML9)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641742.1
- ProteinModelPortal:   Q8PML9
- SMR:   Q8PML9
- GeneID:   1155478
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1407
- NMPDR:   fig|190486.1.peg.1386
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   XAXO190486:XAC1407-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 26526; Mature: 26526

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSTSNGAVVVPATQDGLFHDSPFPIPDSRLIAGVDEAGRGPLAGPVAVAAVVFDPGKP
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHEEEEEECCCCC
RINGLDDSKQLTAERREQLYARIVDRALAWSVVLIDSEEIDRINIYQATMLGMRRAVEGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEHHHHHHHHHHHHHHHHH
AHVAGFARIDGNRVPKGLPCPAEALIGGDALDRAIMAASIVAKVTRDRLMHELHTRHPEY
HHHHHHHEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RFDQHKGYSTPVHLAALQTHGPCPQHRRSFAPVRLALQGREGLEPDPGTRDLEQLQVGQL
CCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
VAPL
HCCC
>Mature Secondary Structure
MRRSTSNGAVVVPATQDGLFHDSPFPIPDSRLIAGVDEAGRGPLAGPVAVAAVVFDPGKP
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHEEEEEECCCCC
RINGLDDSKQLTAERREQLYARIVDRALAWSVVLIDSEEIDRINIYQATMLGMRRAVEGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEHHHHHHHHHHHHHHHHH
AHVAGFARIDGNRVPKGLPCPAEALIGGDALDRAIMAASIVAKVTRDRLMHELHTRHPEY
HHHHHHHEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RFDQHKGYSTPVHLAALQTHGPCPQHRRSFAPVRLALQGREGLEPDPGTRDLEQLQVGQL
CCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
VAPL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217