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The map label for this gene is nerA [H]

Identifier: 21242115

GI number: 21242115

Start: 1569449

End: 1570549

Strand: Reverse

Name: nerA [H]

Synonym: XAC1362

Alternate gene names: 21242115

Gene position: 1570549-1569449 (Counterclockwise)

Preceding gene: 77748581

Following gene: 21242114

Centisome position: 30.35

GC content: 67.3

Gene sequence:

>1101_bases
TTGCTGATGTCCAAATCCACTCAATCCCCCTTGTTCTCCCCGGTGCGCCTGGGCGCACTGGATCTTGCCAACCGCGTGAT
CATGGCCCCGCTGACGCGCAACCGCGCCGTCGCCGGCCAGGTGCCCTCGCCGCTTGCTGCCGAGTACTACGGCCAGCGCG
CCACCGCCGGCCTGATCGTGGCCGAAGGCACCCAGATCAGTCCGCTGGGCCAGGGCTATCTCGATACGCCCGGCATCCAT
ACCAGGCAACAAATCGAGGGCTGGCGCGCTGTCACTGACGAAGTGCATCGTCGCGGCGGCAAGATCGTGCTGCAGCTGTG
GCACGTTGGCCGCGTCTCGCACACCAGTGTGCTGCCCCCGGGCGAAGTGCCGGTTGCGCCCAGCGCCATTCGCGCGGAGG
GCAAGACCTTCACCCCAAACGGGTTCGAAGACGTGTCCGAGCCGCGCGCGCTGGCATTGGACGAAATCCCCGGCTTGATC
GAGGACTACCGCATCGCGGCGCGCAACGCGATCGATGCCGGCTTCGACGGCGTGGAAGTGCATGCGGCCAACGGCTATCT
GCTGGACCAGTTCCTGCGCGACAGCTCCAACAAGCGCACCGACGCCTACGGTGGCGATATCGAAAACCGCACCCGCCTGC
TTGCCGAGGTGGTGCAGGCGATTGCCGATGAAATCGGCGCCGAGCGCACCGGCGTGCGCTTGTCGCCGGTGACCCCGGTG
TACGGCGCGCACGACTCCGACCCGCAGCCGCTGTTCGAACGTGCGGTCGAGCGCCTCAACCTGATCGGCGGGCTGGCTTT
CGTGCACGTGATCGAGGGTGCGACCGGCGGCCCGCGCGACAACATCGCCTTCGACTACGCCGCGCTGCGCGCCAAGTTCG
ACGGCGCCTGGATCGCCAACAACGGCTACGACCGCGGCATGTCCGAACACGCCATCAGCAGCGGCTACGCCGATGCGATC
GCCTATGGCCGCCCGTTCATCGCCAACCCGGACCTGGTGCGTCGCCTGCGCGAGAACGCGCCACTGGCCGAGCTCGACCA
GGCAACGATGTACGGCGGCGGTGCCAAGGGCTATACCGATTACCCCGCATTGCCCGAGTAA

Upstream 100 bases:

>100_bases
TCCTATCTGTATCGCTCGAATCTGCTTGAGTCCACCACCATCGCTACGAAGCACCGCGATCGGCGCGGCGTCGCGCCTGG
CGCGTGATCAAATCGACTGG

Downstream 100 bases:

>100_bases
TCGCCTGATCAGCCCCTGACCACGCGACCGCGCCTGCCGGCTGGCACGGCGCGGTCGCTGTGGGTGAGGCCGATGCACTG
CATGCCTGCTGGTGGCGGAT

Product: GTN reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MLMSKSTQSPLFSPVRLGALDLANRVIMAPLTRNRAVAGQVPSPLAAEYYGQRATAGLIVAEGTQISPLGQGYLDTPGIH
TRQQIEGWRAVTDEVHRRGGKIVLQLWHVGRVSHTSVLPPGEVPVAPSAIRAEGKTFTPNGFEDVSEPRALALDEIPGLI
EDYRIAARNAIDAGFDGVEVHAANGYLLDQFLRDSSNKRTDAYGGDIENRTRLLAEVVQAIADEIGAERTGVRLSPVTPV
YGAHDSDPQPLFERAVERLNLIGGLAFVHVIEGATGGPRDNIAFDYAALRAKFDGAWIANNGYDRGMSEHAISSGYADAI
AYGRPFIANPDLVRRLRENAPLAELDQATMYGGGAKGYTDYPALPE

Sequences:

>Translated_366_residues
MLMSKSTQSPLFSPVRLGALDLANRVIMAPLTRNRAVAGQVPSPLAAEYYGQRATAGLIVAEGTQISPLGQGYLDTPGIH
TRQQIEGWRAVTDEVHRRGGKIVLQLWHVGRVSHTSVLPPGEVPVAPSAIRAEGKTFTPNGFEDVSEPRALALDEIPGLI
EDYRIAARNAIDAGFDGVEVHAANGYLLDQFLRDSSNKRTDAYGGDIENRTRLLAEVVQAIADEIGAERTGVRLSPVTPV
YGAHDSDPQPLFERAVERLNLIGGLAFVHVIEGATGGPRDNIAFDYAALRAKFDGAWIANNGYDRGMSEHAISSGYADAI
AYGRPFIANPDLVRRLRENAPLAELDQATMYGGGAKGYTDYPALPE
>Mature_366_residues
MLMSKSTQSPLFSPVRLGALDLANRVIMAPLTRNRAVAGQVPSPLAAEYYGQRATAGLIVAEGTQISPLGQGYLDTPGIH
TRQQIEGWRAVTDEVHRRGGKIVLQLWHVGRVSHTSVLPPGEVPVAPSAIRAEGKTFTPNGFEDVSEPRALALDEIPGLI
EDYRIAARNAIDAGFDGVEVHAANGYLLDQFLRDSSNKRTDAYGGDIENRTRLLAEVVQAIADEIGAERTGVRLSPVTPV
YGAHDSDPQPLFERAVERLNLIGGLAFVHVIEGATGGPRDNIAFDYAALRAKFDGAWIANNGYDRGMSEHAISSGYADAI
AYGRPFIANPDLVRRLRENAPLAELDQATMYGGGAKGYTDYPALPE

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=370, Percent_Identity=47.027027027027, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1789463, Length=334, Percent_Identity=31.437125748503, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17565138, Length=359, Percent_Identity=29.8050139275766, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=262, Percent_Identity=32.4427480916031, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17559804, Length=310, Percent_Identity=29.0322580645161, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=258, Percent_Identity=31.7829457364341, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=256, Percent_Identity=31.25, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=265, Percent_Identity=28.6792452830189, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=383, Percent_Identity=38.1201044386423, Blast_Score=209, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6321973, Length=385, Percent_Identity=36.1038961038961, Blast_Score=193, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39408; Mature: 39408

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMSKSTQSPLFSPVRLGALDLANRVIMAPLTRNRAVAGQVPSPLAAEYYGQRATAGLIV
CCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCCEECCCCCCCHHHHHHCCCCCCEEEE
AEGTQISPLGQGYLDTPGIHTRQQIEGWRAVTDEVHRRGGKIVLQLWHVGRVSHTSVLPP
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCC
GEVPVAPSAIRAEGKTFTPNGFEDVSEPRALALDEIPGLIEDYRIAARNAIDAGFDGVEV
CCCCCCCHHHHCCCCEECCCCCHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEE
HAANGYLLDQFLRDSSNKRTDAYGGDIENRTRLLAEVVQAIADEIGAERTGVRLSPVTPV
EECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
YGAHDSDPQPLFERAVERLNLIGGLAFVHVIEGATGGPRDNIAFDYAALRAKFDGAWIAN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHCCCEEEEC
NGYDRGMSEHAISSGYADAIAYGRPFIANPDLVRRLRENAPLAELDQATMYGGGAKGYTD
CCCCCCCHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
YPALPE
CCCCCC
>Mature Secondary Structure
MLMSKSTQSPLFSPVRLGALDLANRVIMAPLTRNRAVAGQVPSPLAAEYYGQRATAGLIV
CCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCCEECCCCCCCHHHHHHCCCCCCEEEE
AEGTQISPLGQGYLDTPGIHTRQQIEGWRAVTDEVHRRGGKIVLQLWHVGRVSHTSVLPP
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCC
GEVPVAPSAIRAEGKTFTPNGFEDVSEPRALALDEIPGLIEDYRIAARNAIDAGFDGVEV
CCCCCCCHHHHCCCCEECCCCCHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEE
HAANGYLLDQFLRDSSNKRTDAYGGDIENRTRLLAEVVQAIADEIGAERTGVRLSPVTPV
EECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
YGAHDSDPQPLFERAVERLNLIGGLAFVHVIEGATGGPRDNIAFDYAALRAKFDGAWIAN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHCCCEEEEC
NGYDRGMSEHAISSGYADAIAYGRPFIANPDLVRRLRENAPLAELDQATMYGGGAKGYTD
CCCCCCCHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
YPALPE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]