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The map label for this gene is pcaB [H]

Identifier: 21241143

GI number: 21241143

Start: 439511

End: 440863

Strand: Direct

Name: pcaB [H]

Synonym: XAC0369

Alternate gene names: 21241143

Gene position: 439511-440863 (Clockwise)

Preceding gene: 21241142

Following gene: 21241144

Centisome position: 8.49

GC content: 70.21

Gene sequence:

>1353_bases
ATGAGTGCGACATCGTCGTTGTTGGGATCCTTGTTCGGCGAGCCCGCGTGCGACGCCTTGTTCGACGATGCCGCGCGCTT
GCAGGCGATGCTGCAGTTCGAGTCCGCGCTGGCGCGCGCACAGGCGCAGTGCGGGGTGATTCCCGCCCAGGCCGCGCAGG
CCATTGCCGCTGCCTGCGATGTGCAGCACTACGACCGGGCCGCGTTGGCGCAGGCCACGGCGCTGGCCGGCAATCCCGCC
ATTCCGCTGGTCAAGGCGCTGACCGCGCAGGTGGCCGCGCACGACAATGCCGCCGCGCGCTGGGTGCACTGGGGCGCCAC
CAGCCAGGACGTCATCGATACCGGCACGGTGCTGCAGCTGCGCGCCGCGCTTGATCTGCTGCTGCCGCGTCTGCACGCCC
TGTGCGCCGATCTGGCGGTGCTGGCCGAACGCGAGCGCAACACCGGCTTGCCCGGACGCACCCTGCTGCAGCAGGCCGTG
CCGGTCACCTTCGGGCTCAAGGTCGCCGGTTGGCTGGACGCCCTGCAGCGTGCGCAGCAGCGCCTGCACGCCTTGCGGCG
CGATGCCTTGGTGCTGCAGTTCGGCGGCGCGGCCGGCACCCTGGCCTCGTTGCAGGAGCGTGGCCTGGAAGTGGCGCAGG
CCCTGGCGAGCACTCTGGAGTTGCCGTTGCCCGCGCTGCCCTGGCATACCGCGCGCGACCGCATCGTCGAGGTCGGGTGC
GCTTTCGGTCTGCTGGCTGCCACGTTGGGCAAGATCGGCGGCGATGTGGCGTTGCTGATGCAATCGGAGGTGGGCGAAGC
GTTCGAGCCTGCCGCCGCCGGCAAAGGCGGCTCGTCGGCGATGCCGCACAAGCGCAACCCGGTCTCCAGCGTGGCAGCGG
TTGCCGCAGCCACCCGCGTACCGGGCCTGGTCGCCACATTGTTCAGCGCCATGGCGCAGCCGCACGAGCGCGCGGCCGGG
CAATGGCATGCCGAATGGGACACCTTGCCGCAGATCGTCTGCCTCACCGCCGGCAGCCTGGCGCAGATGCAGCTGTGCCT
GTCCGGTCTGGAACTGGATCGCACGCGCATGCAGGCGCATCTGGACAGCCACGGCGGGCTGCTCTACGCCGAGGCGGCAG
TGTTCGCGCTCGCGTCACACCTGGGCAAGCCGCAGGCGCACGCCCTGGTGGAACAGGCAGCGCTGCGCGCGCAGGCGCAG
CAACGGCATTTGCGCGAGGTGCTCGGCGAAGACGCGCAGGTGAGCGCGGTGCTCACGCACGCGCAACTCCAGGCAGTGTT
CGATAGCGCTAGTTGGCACGGTATGTCCTCGGTCTGGATCGACCGCGTGCTGGCTGGATCTTCTTCCCGTTGA

Upstream 100 bases:

>100_bases
ACCCCGGCAGTGCCAGGCGCGCCGCATGCATTGCCCTTTAGTCTAGTTGGCACCTTGGTACATCGGTGCGATGCTGCCGG
CACGCCTAGAGATGCAATAC

Downstream 100 bases:

>100_bases
CGGTGCTTCGATGGCTTATCTCCAGTTGCCCACGCATCGCCTGCATTACCGCCTGGACGGCACCGAAGGACGCCCCTGGC
TGACCTTCTGCAATTCGCTG

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSATSSLLGSLFGEPACDALFDDAARLQAMLQFESALARAQAQCGVIPAQAAQAIAAACDVQHYDRAALAQATALAGNPA
IPLVKALTAQVAAHDNAAARWVHWGATSQDVIDTGTVLQLRAALDLLLPRLHALCADLAVLAERERNTGLPGRTLLQQAV
PVTFGLKVAGWLDALQRAQQRLHALRRDALVLQFGGAAGTLASLQERGLEVAQALASTLELPLPALPWHTARDRIVEVGC
AFGLLAATLGKIGGDVALLMQSEVGEAFEPAAAGKGGSSAMPHKRNPVSSVAAVAAATRVPGLVATLFSAMAQPHERAAG
QWHAEWDTLPQIVCLTAGSLAQMQLCLSGLELDRTRMQAHLDSHGGLLYAEAAVFALASHLGKPQAHALVEQAALRAQAQ
QRHLREVLGEDAQVSAVLTHAQLQAVFDSASWHGMSSVWIDRVLAGSSSR

Sequences:

>Translated_450_residues
MSATSSLLGSLFGEPACDALFDDAARLQAMLQFESALARAQAQCGVIPAQAAQAIAAACDVQHYDRAALAQATALAGNPA
IPLVKALTAQVAAHDNAAARWVHWGATSQDVIDTGTVLQLRAALDLLLPRLHALCADLAVLAERERNTGLPGRTLLQQAV
PVTFGLKVAGWLDALQRAQQRLHALRRDALVLQFGGAAGTLASLQERGLEVAQALASTLELPLPALPWHTARDRIVEVGC
AFGLLAATLGKIGGDVALLMQSEVGEAFEPAAAGKGGSSAMPHKRNPVSSVAAVAAATRVPGLVATLFSAMAQPHERAAG
QWHAEWDTLPQIVCLTAGSLAQMQLCLSGLELDRTRMQAHLDSHGGLLYAEAAVFALASHLGKPQAHALVEQAALRAQAQ
QRHLREVLGEDAQVSAVLTHAQLQAVFDSASWHGMSSVWIDRVLAGSSSR
>Mature_449_residues
SATSSLLGSLFGEPACDALFDDAARLQAMLQFESALARAQAQCGVIPAQAAQAIAAACDVQHYDRAALAQATALAGNPAI
PLVKALTAQVAAHDNAAARWVHWGATSQDVIDTGTVLQLRAALDLLLPRLHALCADLAVLAERERNTGLPGRTLLQQAVP
VTFGLKVAGWLDALQRAQQRLHALRRDALVLQFGGAAGTLASLQERGLEVAQALASTLELPLPALPWHTARDRIVEVGCA
FGLLAATLGKIGGDVALLMQSEVGEAFEPAAAGKGGSSAMPHKRNPVSSVAAVAAATRVPGLVATLFSAMAQPHERAAGQ
WHAEWDTLPQIVCLTAGSLAQMQLCLSGLELDRTRMQAHLDSHGGLLYAEAAVFALASHLGKPQAHALVEQAALRAQAQQ
RHLREVLGEDAQVSAVLTHAQLQAVFDSASWHGMSSVWIDRVLAGSSSR

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17508577, Length=348, Percent_Identity=24.7126436781609, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323391, Length=240, Percent_Identity=28.3333333333333, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24647570, Length=327, Percent_Identity=28.7461773700306, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 47132; Mature: 47001

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATSSLLGSLFGEPACDALFDDAARLQAMLQFESALARAQAQCGVIPAQAAQAIAAACD
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VQHYDRAALAQATALAGNPAIPLVKALTAQVAAHDNAAARWVHWGATSQDVIDTGTVLQL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHEEECCCCCCCHHCCCHHHHH
RAALDLLLPRLHALCADLAVLAERERNTGLPGRTLLQQAVPVTFGLKVAGWLDALQRAQQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RLHALRRDALVLQFGGAAGTLASLQERGLEVAQALASTLELPLPALPWHTARDRIVEVGC
HHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AFGLLAATLGKIGGDVALLMQSEVGEAFEPAAAGKGGSSAMPHKRNPVSSVAAVAAATRV
HHHHHHHHHHHCCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PGLVATLFSAMAQPHERAAGQWHAEWDTLPQIVCLTAGSLAQMQLCLSGLELDRTRMQAH
CHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LDSHGGLLYAEAAVFALASHLGKPQAHALVEQAALRAQAQQRHLREVLGEDAQVSAVLTH
HHCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AQLQAVFDSASWHGMSSVWIDRVLAGSSSR
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SATSSLLGSLFGEPACDALFDDAARLQAMLQFESALARAQAQCGVIPAQAAQAIAAACD
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VQHYDRAALAQATALAGNPAIPLVKALTAQVAAHDNAAARWVHWGATSQDVIDTGTVLQL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHEEECCCCCCCHHCCCHHHHH
RAALDLLLPRLHALCADLAVLAERERNTGLPGRTLLQQAVPVTFGLKVAGWLDALQRAQQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RLHALRRDALVLQFGGAAGTLASLQERGLEVAQALASTLELPLPALPWHTARDRIVEVGC
HHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AFGLLAATLGKIGGDVALLMQSEVGEAFEPAAAGKGGSSAMPHKRNPVSSVAAVAAATRV
HHHHHHHHHHHCCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PGLVATLFSAMAQPHERAAGQWHAEWDTLPQIVCLTAGSLAQMQLCLSGLELDRTRMQAH
CHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LDSHGGLLYAEAAVFALASHLGKPQAHALVEQAALRAQAQQRHLREVLGEDAQVSAVLTH
HHCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AQLQAVFDSASWHGMSSVWIDRVLAGSSSR
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9582432; 12597275 [H]