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The map label for this gene is truC [H]

Identifier: 21241018

GI number: 21241018

Start: 292046

End: 292780

Strand: Direct

Name: truC [H]

Synonym: XAC0244

Alternate gene names: 21241018

Gene position: 292046-292780 (Clockwise)

Preceding gene: 21241017

Following gene: 21241020

Centisome position: 5.64

GC content: 64.76

Gene sequence:

>735_bases
GTGAGCATATCCCCCAAACATCTGCAGATTCTCTACCAGGACGATGTCCTGGCCGTCGTCGACAAACCCGCCGGCCTGAT
GGTCCATGACAGCAAGCTGGCGCGTGGAGAGGACGACTTCCTGGCCGACCGCCTGCGCGAGCAGCTGGGCAAGCCGATCT
TTTTGGTGCACCGGCTCGACCGCGCCACCAGCGGCTGCCTGCTGTTGGCCTTCGATCGCGACAGCGCCAGTGCGCTGGGC
AAGGCTTTGATGGCCGGCGAGGTGGAGAAACAGTATCTGACCGTCTGTCGCGGCTGGCCGGCCGAGGAGCGCTTCATCGT
CGACCATGACCTGGACGGCGGTCCGGGCAAGCCGGTCAAGAAGCCGGCAGTGACGCATTTCCAGCGTCTGGCCACCGGCG
AGCTGGAGATTCCTTCCACCGGGTTCACCACCTCGCGCTACGCACTGCTGCGCTGTCAGCCGCAAACCGGCCGCTTCCGG
CAGATTCGCCGGCATATGAAGCATCTGTCGCATCACATGGTCGGCGACACCAGCCACGGCGACGGCCGCCACAACCGCAG
TTTCCGCATGCTCGGCATCCACCGCATGTTGCTGCACGCCGAACGCCTGGAATTTCCGCATCCGGCCGATGGCAGGCGCA
TCGGCGTCACCGCGCCGCTGGATGTCGAATTTTCCAAGGCCTGCGCGTTGTTTGGGTGGGAGATGGCGGGGTTGGGAGTC
ATGCAGACGGCGTGA

Upstream 100 bases:

>100_bases
GTTGCCGTGCGCGTGGCCTGGCGACGACACAGTCGTTTGTTCGCTGCGGCAGCGCGGCGATGCAGTGGCATGCGGTGTCG
GCGATGCGGATAATTCGCCG

Downstream 100 bases:

>100_bases
TGTGGCGTGCCTGGTTCGCAGATCGAGACGTTCACTCAGTCTGCTAGACATCTGCGCAAACGTCCCCTCACCCTAACCCC
TCTCCCGCCGGCGGGAGAGG

Product: pseudouridylate synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MSISPKHLQILYQDDVLAVVDKPAGLMVHDSKLARGEDDFLADRLREQLGKPIFLVHRLDRATSGCLLLAFDRDSASALG
KALMAGEVEKQYLTVCRGWPAEERFIVDHDLDGGPGKPVKKPAVTHFQRLATGELEIPSTGFTTSRYALLRCQPQTGRFR
QIRRHMKHLSHHMVGDTSHGDGRHNRSFRMLGIHRMLLHAERLEFPHPADGRRIGVTAPLDVEFSKACALFGWEMAGLGV
MQTA

Sequences:

>Translated_244_residues
MSISPKHLQILYQDDVLAVVDKPAGLMVHDSKLARGEDDFLADRLREQLGKPIFLVHRLDRATSGCLLLAFDRDSASALG
KALMAGEVEKQYLTVCRGWPAEERFIVDHDLDGGPGKPVKKPAVTHFQRLATGELEIPSTGFTTSRYALLRCQPQTGRFR
QIRRHMKHLSHHMVGDTSHGDGRHNRSFRMLGIHRMLLHAERLEFPHPADGRRIGVTAPLDVEFSKACALFGWEMAGLGV
MQTA
>Mature_243_residues
SISPKHLQILYQDDVLAVVDKPAGLMVHDSKLARGEDDFLADRLREQLGKPIFLVHRLDRATSGCLLLAFDRDSASALGK
ALMAGEVEKQYLTVCRGWPAEERFIVDHDLDGGPGKPVKKPAVTHFQRLATGELEIPSTGFTTSRYALLRCQPQTGRFRQ
IRRHMKHLSHHMVGDTSHGDGRHNRSFRMLGIHRMLLHAERLEFPHPADGRRIGVTAPLDVEFSKACALFGWEMAGLGVM
QTA

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI17158025, Length=240, Percent_Identity=30, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI23308689, Length=181, Percent_Identity=28.1767955801105, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221316667, Length=224, Percent_Identity=27.6785714285714, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1789155, Length=241, Percent_Identity=38.5892116182573, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1787327, Length=245, Percent_Identity=34.2857142857143, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786244, Length=216, Percent_Identity=32.4074074074074, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788946, Length=234, Percent_Identity=28.6324786324786, Blast_Score=71, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320168, Length=187, Percent_Identity=28.3422459893048, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324506, Length=188, Percent_Identity=28.1914893617021, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321608, Length=201, Percent_Identity=26.3681592039801, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 27277; Mature: 27146

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISPKHLQILYQDDVLAVVDKPAGLMVHDSKLARGEDDFLADRLREQLGKPIFLVHRLD
CCCCCCCEEEEEECCEEEEEECCCCEEEECCHHCCCCHHHHHHHHHHHHCCCEEEEEECC
RATSGCLLLAFDRDSASALGKALMAGEVEKQYLTVCRGWPAEERFIVDHDLDGGPGKPVK
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCC
KPAVTHFQRLATGELEIPSTGFTTSRYALLRCQPQTGRFRQIRRHMKHLSHHMVGDTSHG
CHHHHHHHHHHCCCEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCC
DGRHNRSFRMLGIHRMLLHAERLEFPHPADGRRIGVTAPLDVEFSKACALFGWEMAGLGV
CCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCEE
MQTA
ECCC
>Mature Secondary Structure 
SISPKHLQILYQDDVLAVVDKPAGLMVHDSKLARGEDDFLADRLREQLGKPIFLVHRLD
CCCCCCEEEEEECCEEEEEECCCCEEEECCHHCCCCHHHHHHHHHHHHCCCEEEEEECC
RATSGCLLLAFDRDSASALGKALMAGEVEKQYLTVCRGWPAEERFIVDHDLDGGPGKPVK
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCC
KPAVTHFQRLATGELEIPSTGFTTSRYALLRCQPQTGRFRQIRRHMKHLSHHMVGDTSHG
CHHHHHHHHHHCCCEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCC
DGRHNRSFRMLGIHRMLLHAERLEFPHPADGRRIGVTAPLDVEFSKACALFGWEMAGLGV
CCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCEE
MQTA
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]